| 1. |
- Chorell, Elin, et al.
(författare)
-
Impact of probiotic feeding during weaning on the serum lipid profile and plasma metabolome in infants
- 2013
-
Ingår i: British Journal of Nutrition. - : Cambridge University Press. - 0007-1145 .- 1475-2662. ; 110:1, s. 116-126
-
Tidskriftsartikel (refereegranskat)abstract
- The gut microbiome interacts with the host in the metabolic response to diet, and early microbial aberrancies may be linked to the development of obesity and metabolic disorders later in life. Probiotics have been proposed to affect metabolic programming and blood lipid levels, although studies are lacking in infants. Here, we report on the lipid profile and global metabolic response following daily feeding of probiotics during weaning. A total of 179 healthy, term infants were randomised to daily intake of cereals with (n 89) or without (n 90) the addition of Lactobacillus paracasei ssp. paracasei F19 (LF19) 108 colony-forming units per serving from 4 to 13 months of age. Weight, length and skinfold thickness were monitored. Venous blood was drawn at 5·5 and 13 months of age for analysis of the serum lipid profile. In a subsample, randomly selected from each group, GC-time-of-flight/MS was used to metabolically characterise plasma samples from thirty-seven infants. A combination of multi- and univariate analysis was applied to reveal differences related to LF19 treatment based on 228 putative metabolites, of which ninety-nine were identified or classified. We observed no effects of probiotic feeding on anthropometrics or the serum lipid profile. However, we detected significantly lower levels of palmitoleic acid (16 : 1) (P < 0·05) and significantly higher levels of putrescine (P < 0·01) in LF19-treated infants. Palmitoleic acid is a major MUFA strongly linked to visceral obesity, while putrescine is a polyamine with importance for gut integrity. Whether the observed differences will have long-term health consequences are being followed.
|
|
| 2. |
|
|
| 3. |
- Karlsson Videhult, Frida, 1980-, et al.
(författare)
-
The plasma metabolome is influenced by body weight and sex already at school age
-
Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Diet is one of the determinants of gut microbial composition. Reported changes in the biodiversity of the gut microbiota in the obese have spurred interest in gut microbiota modulation by dietary interventions. Using an untargeted metabolomics approach, we previously reported that infant cereals with the probiotic Lactobacillus paracasei ssp. paracasei F19 (LF19) fed daily from 4 to 13 months of age affected the plasma metabolome with lower levels of fatty acids associated with obesity indices compared with placebo. The study participants were invited to a follow-up study at 8-9 years of age and 120 children participated. Venous blood was drawn after overnight fasting and plasma samples were available from 112 children. Samples were analysed using GCxGC-time-of-flight/MS for characterisation of the global plasma metabolome. A combination of multivariate and univariate analysis was used to reveal differences between the LF19 and placebo group, and according to weight class and sex. The lower levels of palmitic acid and palmitoleic acid in the LF19 group during the intervention did not remain at the follow-up. Stronger models according to weight class and sex were obtained. BMI was associated with several metabolites including the branched-chain amino acids leucine and isoleucine, and the aromatic amino acids, tyrosine and phenylalanine. Collectively, feeding LF19 during weaning induced transient effects on the plasma metabolome. The disparities seen in the metabolic profile of overweight/obese young school children underscore the need for effective early preventive and treatment strategies.
|
|
