| 1. |
- Fedkiv, Olexandr, et al.
(författare)
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Growth is dependent on the exocrine pancreas function in young weaners but not in growing-finishing pigs
- 2009
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Ingår i: Journal of Physiology and Pharmacology. - 0867-5910. ; 60:Suppl. 3, s. 55-59
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Konferensbidrag (refereegranskat)abstract
- A correlation between the exocrine pancreatic function and growth has been previously demonstrated in growing pigs but the data are inconsistent. This was investigated by studying the growth performance of pigs with exocrine pancreatic insufficiency (EPI) at different ages and maintained under similar conditions. Twelve 7 week old (10.5 +/- 1.3 kg) weaners, and twelve 16 week old (43 +/- 5 kg) growing-finishing pigs were used in the experiments, and 6 pigs from each group were operated and pancreatic duct-ligated. Starting at 3-5 weeks after the operation, when EPI had developed, weekly recordings of feed consumption and growth were done before, during and after feed supplementation with porcine pancreatin (Creon (R) 10000). In weaner pigs, EPI caused growth arrest while it did not affect the growth of older pigs, as compared to respective un-operated groups of pigs. The daily feed consumption (DFC) was lower in the weaner EPI-pigs while it was similar in the growing-finishing EPI-pigs, as compared to un-operated pigs. Feed supplementation with Creon (R) improved the DFC and growth in both the EPI and un-operated pigs. In conclusion, the results showed the importance of the exocrine pancreatic function for growth in weaner pigs, while in older animals it played a minor role in growth. Feed supplementation with pancreatin increased the appetite and ensured an improved feed conversion.
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| 2. |
- Köhnke, Rickard, et al.
(författare)
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Feeding appetite suppressing thylakoids to pigs alters pancreatic lipase/colipase secretion
- 2010
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Ingår i: Livestock Science. - : Elsevier BV. - 1871-1413. ; 134:1-3, s. 68-71
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Konferensbidrag (refereegranskat)abstract
- The mechanism for a new appetite suppressor named thylakoids (membrane proteins derived from spinach leaves) was examined in vivo in pigs. Thylakoids inhibit the lipase/colipase hydrolysis of triacylglycerols (TG) in vitro and suppress food intake, decrease body weight gain and raise the circulating satiety hormone cholecystokinin (CCK) in rats but its mechanism in vivo remains unclear. We hypothesized that a thylakoid-enriched diet prolongs intestinal digestion of food and therefore promote satiety signaling. Five pigs were surgically prepared with a fistula in the duodenum for collection of digesta and with two catheters, one in v. jugularis and one in v. porta, for blood collection. After 1 week of recovery and an overnight fast the pigs were fed a high-fat diet with and without supplementation with thylakoids. Duodenal content and blood samples were taken before and 15, 30, 60, 120, 240 and 360 min after feeding. Pancreatic lipase and colipase enzymes were measured in duodenal digesta. Blood samples were analyzed for the satiety hormone CCK as well as insulin and glucose. We found that pancreatic lipase/colipase level increased and stayed elevated for a longer time in the duodenum in the pigs receiving thylakoids compared to the control. CCK levels were unchanged. Insulin levels were significantly reduced by the thylakoid treatment without any change in blood glucose. In conclusion, thylakoids increased lipase/colipase secretion. The mechanism for this secretion appears not to be related to CCK and may be an effect of vagal activation. Thylakoids gave reduced insulin levels without any change in glucose levels. (C) 2010 Published by Elsevier B.V.
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| 3. |
- Rengman, Sofia, et al.
(författare)
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The growth of exocrine pancreatic insufficient young pigs fed an elemental diet is dependent on enteral pancreatin supplementation
- 2010
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Ingår i: Livestock Science. - : Elsevier BV. - 1871-1413 .- 1878-0490. ; 134:1-3, s. 50-52
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Konferensbidrag (refereegranskat)abstract
- Young exocrine pancreas insufficient (EPI) pigs given a commercial feed (polymeric diet) show growth retardation that can be reversed by dietary supplementation with pancreatic enzymes. Our aim was to investigate if providing an elemental diet, mimicking a pre-digested diet, can support body growth in EPI-pigs. Thus, EPI-pigs, pancreatic duct-ligated at 12.2 +/- 2.6 weeks of age (15.3 +/- 4.8 kg), were fed for 6 days either a commercial polymeric pig feed or infused i.v. with an elemental diet, with or without oral supplementation with a porcine enzyme preparation (Creon (R)). The body weight (BW) gain was then compared at day 7. Control pigs (with intact pancreata) showed a 14.0 +/- 1.3% increase in BW independent of the diet, while EPI-pigs given the same diets either lost (polymeric diet) or slightly increased (elemental diet) their BW. In contrast, EPI-pigs fed the polymeric feed with Creon supplementation showed a normalised growth and EPI-pigs given the elemental diet with Creon supplementation gained 8.5 +/- 0.7% in BW. In conclusion, control pigs maintained a normal growth, independently of the diet being given in polymeric or elemental form, while EPI-pigs showed an impaired growth when receiving the same diets without oral enzyme supplementation. This suggests that pancreatic juice or enzyme preparations, in addition to their digestive properties, stimulate nutrient assimilation and anabolic processes in young fast-growing pigs. (C) 2010 Elsevier B.V. All rights reserved.
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| 4. |
- Tannergren, Christer, et al.
(författare)
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The effect of pancreatic and biliary depletion on in vivo pharmacokinetics of digoxin in pigs
- 2006
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Ingår i: Advances in Understanding Oral Absorption and Delivery of Problem Compounds - Selected Papers from the 3rd World Conference on Drug Absorption, Transport and Delivery (European Journal of Pharmaceutical Sciences). - : Elsevier BV. - 1879-0720 .- 0928-0987. ; 29:3-4, s. 198-204
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Konferensbidrag (refereegranskat)abstract
- Several transporter systems in the liver and intestine are known to change their expression and function during cholestatic disease states. The objective of the-present in vivo study-was to investigate the effect of biliary depletion, as a method to mimic cholestasis, on the bioavailability and disposition of digoxin in biliary and pancreatic duct cannulated pigs. The study was divided in two parts. In the first part, a solution of 10 mu g/kg digoxin was administered intravenously to the cannulated pigs with intact enterohepatic circulation (Control) and during depletion of the bile and pancreatic juice. In the second part, the same dose of digoxin was adminstered intraduodenally with intact enterohepatic circulation (Control) and during depletion of either bile or pancreatic juice or both. Biliary depletion decreased the flow of bile and pancreas juice as well as the amount of digoxin appearing in the bile. Deprivation of both bile and pancreas juice significantly increased the bioavailability of digoxin, the plasma AUC after enteral administration increased from 17.6 +/- 4.2 nmol/lh (Control) to 29.6 +/- 8.3,nmol/lh (P < 0.05). The biliary clearance decreased significantly, from 0.22 +/- 10.11 l/h/kg (Control) to 0.04 +/- 0.03 l/h/kg during pancreatic and biliary depletion (P < 0.05). There was a significant decrease in elimination half-life (P < 0.05) and volume of distribution (P < 0.01) during the depletion experiments while the systemic clearance remained unchanged. The results clearly suggest that biliary depletion trigger a short-term downregulation, most likely posttranscriptionally mediated, of a sinusoidal uptake transporter in the liver, possibly a pig ortholog of OATP. (c) 2006 Elsevier B.V. All rights reserved.
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