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| 2. |
- Kruszewska, Danuta, et al.
(författare)
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Enteral crude red kidney bean (Phaseolus vulgaris) lectin--phytohemagglutinin--induces maturational changes in the enterocyte membrane proteins of suckling rats.
- 2003
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Ingår i: Biology of the Neonate. - : S. Karger AG. - 1421-9727. ; 84:2, s. 152-158
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Tidskriftsartikel (refereegranskat)abstract
- This study aimed to investigate the effect of enterally administered crude red kidney bean (Phaseolus vulgaris) lectin, PHA, on the expression of brush-border membrane vesicle (BBMV) proteins, in particular Na+/H+ exchangers (NHEs), in the small intestine of suckling rats. Gavage of PHA to 14-day-old rats for 3 days resulted in altered protein/glycoprotein patterns as analyzed by SDS-PAGE. Immunoblots demonstrated the appearance of two 71- and 27-kD protein bands indicative for NHE3 - one of the NHE isoforms - and PHA, respectively. PHA treatment also resulted in an augmented uptake of 22Na+ by the BBMV indicating an increase in NHE activity. Overall, the data suggests that enteral PHA exposure may induce maturational changes in enterocyte membrane proteins in young rats. In view of these findings, an investigation into the addition of PHA to infant formulas and weaning diets is warranted.
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| 3. |
- Pierzynowski, Stefan, et al.
(författare)
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Behavioral changes in response to feeding pancreatic-like enzymes to exocrine pancreatic insufficiency pigs.
- 2012
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Ingår i: Journal of Animal Science. - : Oxford University Press (OUP). - 1525-3163 .- 0021-8812. ; 90:Suppl 4, s. 439-441
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Tidskriftsartikel (refereegranskat)abstract
- Behavioral changes during pancreatic enzyme therapy have never been studied. The present study investigated behavioral changes in exocrine pancreatic insufficiency (EPI) pigs when their feed was supplemented with pancreatic-like enzymes of microbial origin. A crossover design study was used to test the effect of enzyme supplementation in 2 × 4 EPI pigs that underwent pancreatic duct ligation (PDL). After 40 d of adaptation, the study commenced, comprising 2 control and 2 enzyme feeding periods of 10 d each in sequence. On days 7 and 10 of each experimental period, behavior was monitored for 24 h and feed consumption and BW were recorded. Behavioral observations focused on the pigs' activity- lying down or passive, or sitting, or standing or active-and were expressed as percentage activity for 24 h. During the adaptation period, BW gain was completely inhibited after PDL whereas for the entire study period, the body weight increased from 10.5 ± 1.1 to 14.0 ± 1.4 kg (P < 0.01). Exocrine pancreatic insufficiency pigs were more active when fed the enzymes (21 vs. 18% per 24 h; P < 0.01). Microbial enzyme supplementation not only improved the growth of the EPI pigs but it also increased their activity. This behavior change contradicts the generally accepted norm that satiety evokes by digestion and subsequent nutrients absorption reduces human or animal motility.
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| 4. |
- Pierzynowski, Stefan, et al.
(författare)
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Real exposure to pig pancreatic juice and bile inhibit exocrine pancreatic secretion in pigs
- 2007
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Ingår i: Livestock Science. - : Elsevier BV. - 1871-1413. ; 108:1-3, s. 53-56
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Tidskriftsartikel (refereegranskat)abstract
- The mechanisms involved in the down regulation of the exocrine pancreas are still not fully elucidated. The purpose of the present study was to examine the effect of duodenal and ileal exposure to pancreatic enzymes and bile on pancreatic secretion. Experiments were performed with pigs fitted with pancreatic duct, ileal and jugular vein catheters, and duodenal T-shaped cannula. A negative feedback regulation of pancreatic secretion was found for both duodenal and ileal delivery of pancreatic juice. Pancreatic secretion was unaffected by duodenal delivery of bile and bile salts, while ileal delivery of bile and bile salts lowered it. On the other hand, pancreatic secretion was increased by duodenal loading of commercial non- or activated pancreatic enzyme preparations, thus probably recognised as dietary protein. Plasma cholecystokinin (CCK) level was lowered only by duodenal delivery of pancreatic juice. In conclusion, the results prove the existence of negative feedback mechanism regulating pancreatic secretion dependent on the presence of pure pancreatic juice or bile at the ileal level. The mechanism at the duodenal level is exclusively sensitive to pure pancreatic juice. (c) 2007 Elsevier B.V All rights reserved.
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| 5. |
- Pierzynowski, Stefan, et al.
(författare)
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Relations between pig growth and regulatory mechanism of pancreas - facts and hypotheses
- 2005
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Ingår i: Journal of Animal and Feed Sciences. - 1230-1388. ; 14:Suppl 1, s. 139-144
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Tidskriftsartikel (refereegranskat)abstract
- The aim of the mini review is to more precisely determine the relationship between exocrine pancreas function and the growth of young pigs. In order to do so, it is necessary to clarify the components, pathways and messengers involved in the CCK-dependent neuro-hormonal regulation of the exocrine pancreas in the growing pigs. These mechanisms are largely unknown, and the role of CCK has been questioned since CCK receptors on the enzyme producing acinar cells are lacking. Our hitherto obtained results have shown the importance of CCK for the pancreatic enzyme secretion and that in pigs CCK functions via CCK-B receptor-mediated entero(duodenal)-pancreatic reflex and via CCK-A receptor mechanism which localization is unknown. Preliminary studies have indicated this later mechanism as being related to the growth performance of pigs. The planned studies will comprise a pharmacologic characterization and an immunohistologic localization of the components and messengers involved in the CCK-dependent neuro-hormonal pancreatic regulation using an appropriately catheterized pig model. Since the applied aim of the project is to correlate the growth of the pigs to the effectiveness of the pancreatic secretion and to the development of its regulatory mechanisms, the experiments will be performed on pigs having different growth and performance. It is expected that the discussion will provide important applicable understanding of pancreatic function and its impact on pig growth and performance and also highlight the mechanism of the inter-organ regulatory pathways of the exocrine pancreatic function.
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| 6. |
- Sileikiene, Vaida, et al.
(författare)
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Effect of ileal infusion of short-chain fatty acids on pancreatic prandial secretion and gastrointestinal hormones in pigs
- 2008
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Ingår i: Pancreas. - 0885-3177. ; 37:2, s. 196-202
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Tidskriftsartikel (refereegranskat)abstract
- Objectives: Nutrients passing the ileum induce mechanisms regulating pancreatic secretion, but the effect of short-chain fatty acids (SCFAs) present in the ileum because of either intestinal fermentation or due to the cecoileal reflux is still unclear. This study investigated the effect of ileal SCFAs on pancreatic secretion and plasma levels of peptide YY, cholecystokinin, motilin, and neurotensin. Methods: The pigs were fitted with pancreatic duct, ileal, and jugular vein catheters, and a duodenal T-shaped cannula. Saline, 85.0 or 170.0 mM acetate, 5.0 or 10.0 mM butyrate, 7.5 or 15.0 mM propionate were infused into the ileum during feeding. Results: The ileal infusions of SCFAs did not affect the pancreatic juice outflow and the lipase output. The protein output was lower when 10.0 mM butyrate or 170.0 mM acetate were infused. The trypsin output decreased for most of the SCFA infusions. The alpha-amylase output decreased for the infusion of 10.0 mM butyrate and tended to decrease for 170.0 mM acetate. The infusions did not change gut hormone level. Conclusions: Ileal SCFAs might induce an inhibition of pancreatic enzyme secretion under prandial conditions. Ileal SCFAs do not inhibit pancreatic secretion by a hormonal pathway involving the release of peptide YY, motilin, neurotensin, or cholecystokinin.
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| 7. |
- Sileikiene, V, et al.
(författare)
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Effect of short chain fatty acids infused intraileally on interdigestive exocrine pancreatic secretions in growing pigs
- 2005
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Ingår i: Journal of Animal Physiology and Animal Nutrition. - : Wiley. - 0931-2439 .- 1439-0396. ; 89:7-8, s. 253-259
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Tidskriftsartikel (refereegranskat)abstract
- The effect of intraileally infused short chain fatty acids (SCFA) and saline as control on the exocrine pancreatic secretions during the interdigestive phase was studied using three 8-weeks-old piglets. Pigs were surgically fitted with a pancreatic duct catheter, re-entrant duodenal T-cannula for collection and subsequent return of pancreatic juice, and with an infusion T-cannula at the distal ileum. Saline as control, 5.0 and 10.0 mm butyrate, 7.5 and 15.0 mm propionate and 85.0 and 170.0 mm acetate were infused at 2 ml/kg body weight (BW) for 30 min into the ileum of overnight fasted piglets via ileal T-cannula. The calculated volume of infusates was administrated in five equal bolus at 6 min intervals over a period of 30 min. The pancreatic juice was collected 60 and 30 min before and 30, 60, 90 and 120 min after the start of infusion. The trypsin ( p = 0.07, p > 0.15 respectively) and protein ( p > 0.15, p = 0.05 respectively) outputs immediately decreased after the infusion of acetate at the dose of 85.0 and 170.0 mm, respectively, whereas pancreatic juice outflow ( p > 0.15) was not significantly affected when compared with levels 30 min before infusion. After the infusion of butyrate at the dose of 5.0 mm, trypsin ( p = 0.01) and protein ( p = 0.12) outputs increased immediately whereas pancreatic juice outflow was not affected ( p > 0.15) in comparison with levels 30 min before infusion. No significant differences were observed after infusion of butyrate at the dose of 10 mm for the pancreatic juice outflow, trypsin and protein outputs when compared with the level before infusion, although these values were numerically lower immediately after the infusion. The pancreatic juice outflow increased ( p = 0.03) after the infusion of propionate at the dose of 7.5 mm and decreased ( p = 0.005) immediately after the infusion of propionate at the dose of 15.0 mm when compared with the levels 30 min before the infusions. After the infusion of propionate at the dose of 7.5 or 15.0 mm for the output of protein and trypsin, no significant differences ( p > 0.15) were observed when compared with levels 30 min before infusion. In summary, the intraileal infusion of SCFA at different doses exerts a short-term and moderate effect on the interdigestive exocrine pancreatic secretions in pigs.
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| 8. |
- van den Borne, Jost, et al.
(författare)
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Exocrine pancreatic secretion in pigs fed sow's milk and milk replacer, and its relationship to growth performance
- 2007
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Ingår i: Journal of Animal Science. - : Oxford University Press (OUP). - 1525-3163 .- 0021-8812. ; 85:2, s. 404-412
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Tidskriftsartikel (refereegranskat)abstract
- The objective of this study was to quantify and compare the effects of sow’s milk and 2 milk replacer diets (containing clotting or nonclotting protein sources) on exocrine pancreatic secretion, plasma cholecystokinin, and immunoreactive cationic trypsin in pigs. In addition, the relationship between exocrine pancreatic secretion and growth in milk-fed pigs was studied. In a changeover experiment, 9 chronically catheterized pigs of 6.6 ± 0.19 kg of BW were studied for 3 wk. Pigs were assigned to each of 3 diets. Exocrine pancreatic secretion was measured from the third to the seventh day on each diet. The protein content and trypsin activity of the pancreatic juice were measured. Blood samples were taken at 10 min before and after milk ingestion and were analyzed for cholecystokinin and immunoreactive cationic trypsin. Pancreatic protein and trypsin secretion did not differ between pigs fed sow’s milk and those fed milk replacer, but the volume secreted was less for the pigs fed sow’s milk (0.75 vs. 1.03 mL·kg–1·h–1; P < 0.01). A postprandial response to milk intake was not observed. The 2 milk replacer diets did not affect exocrine pancreatic secretion differently. The average exocrine pancreatic secretion (volume, 0.94 mL·kg–1·h–1; protein, 4.28 mg·kg–1·h–1; trypsin, 1.65 U·kg–1·h–1) was intermediate between literature values for suckling and weaned pigs. Plasma cholecystokinin was elevated (18 pmol·L–1) and showed low correlations with the pancreatic secretion traits. Plasma immunoreactive cationic trypsin was not significantly related to any of the pancreatic secretion traits and should therefore not be used as an indicator for exocrine pancreatic function in milk-fed pigs. Exocrine pancreatic secretion varied substantially among individual pigs (protein, 0.22 to 13.98 mg·kg–1·h–1). Pancreatic protein and trypsin secretion showed a positive, nonlinear relationship with performance traits. It was concluded that neither specific sow’s milk ingredients nor the protein source are responsible for a low pancreatic protein secretion in suckling pigs. Exocrine pancreatic secretion was positively correlated with ADG in pigs at an identical milk intake.
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