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| 2. |
- Lauvrud, Anne Therese, et al.
(författare)
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Water jet-assisted lipoaspiration and Sepax cell separation system for the isolation of adipose stem cells with high adipogenic potential
- 2021
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Ingår i: Journal of Plastic, Reconstructive & Aesthetic Surgery. - : Elsevier. - 1748-6815 .- 1878-0539. ; 74:10, s. 2759-2767
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Water jet-assisted liposuction has gained popularity due to favourable fat grafting outcomes. In this study, we compared stem cells obtained from fat isolated with manual or the water jet-assisted procedure.Methods: Liposuction of abdominal fat was performed using the two methods on each donor (n = 10). Aspirate samples were collagenase digested and the isolated cells seeded in vitro prior to proliferation, adipogenic differentiation and angiogenic activity analyses.Results: Cells from either procedure proliferated at similar rates and exhibited a similar colony-forming ability. The cells expressed stem cell markers CD73, CD90 and CD105. In the water jet cell preparations, there were higher numbers of cells expressing CD146. Robust adipogenic differentiation was observed in cultures expanded from both manual and water jet lipoaspirates. Gene analysis showed higher expression of the adipocyte markers aP2 and GLUT4 in the adipocyte-differentiated water jet cell preparations, and ELISA indicated increased secretion of adiponectin from these cells. Both cell groups expressed vasculogenic factors and the water jet cells promoted the highest levels of in vitro angiogenesis. Given these positive results, we further characterised the water jet cells when prepared using an automated closed cell processing unit, the Sepax-2 system (Cytiva). The growth and stem cell properties of the Sepax-processed cells were similar to the standard centrifugation protocol, but there was evidence for greater adipogenic differentiation in the Sepax-processed cells.Conclusions: Water jet lipoaspirates yield cells with high adipogenic potential and angiogenic activity, which may be beneficial for use in cell-assisted lipotransfers.
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- McGrath, Aleksandra M., et al.
(författare)
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Long-Term Effects of Fibrin Conduit with Human Mesenchymal Stem Cells and Immunosuppression after Peripheral Nerve Repair in a Xenogenic Model
- 2018
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Ingår i: Cell Medicine. - : SAGE Publications. - 2155-1790. ; 10, s. 1-13
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Tidskriftsartikel (refereegranskat)abstract
- Introduction: Previously we showed that a fibrin glue conduit with human mesenchymal stem cells (hMSCs) and cyclosporine A (CsA) enhanced early nerve regeneration. In this study long term effects of this conduit are investigated. Methods: In a rat model, the sciatic nerve was repaired with fibrin conduit containing fibrin matrix, fibrin conduit containing fibrin matrix with CsA treatment and fibrin conduit containing fibrin matrix with hMSCs and CsA treatment, and also with nerve graft as control. Results: At 12 weeks 34% of motoneurons of the control group regenerated axons through the fibrin conduit. CsA treatment alone or with hMSCs resulted in axon regeneration of 67% and 64% motoneurons respectively. The gastrocnemius muscle weight was reduced in the conduit with fibrin matrix. The treatment with CsA or CsA with hMSCs induced recovery of the muscle weight and size of fast type fibers towards the levels of the nerve graft group. Discussion: The transplantation of hMSCs for peripheral nerve injury should be optimized to demonstrate their beneficial effects. The CsA may have its own effect on nerve regeneration.
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| 4. |
- Wiberg, Britt, et al.
(författare)
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A qualitative and quantitative study of seven persons with balanced time perspective (BTP) according to S-ZTPI
- 2012
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Ingår i: 1st international conference on time perspective and research. - : ESPACOBRANCO. ; , s. 120-120
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Konferensbidrag (refereegranskat)abstract
- The theoretical notion of balanced time perspective (BTP) has been suggested by a number of authors and some attempts to operationalize BTP (Boniwell, 2005; Drake, Duncan, Sutherland, Abernethy & Henry, 2008; Sircova & Mitina, 2008; Boniwell, Osin, Linley & Ivanchenko, 2010) has been done. The aim of the present study was to get a deeper understanding of the BTP-concept by studying seven BTP-persons with both interviews and self-rating scales (e.g. SCL-90, Life Events scale, Scales of Psychological Well-Being (C. Ryff) and Satisfaction with Life Scale (E.Diener) at two occasions. Swedish Zimbardo Time Perspective Inventory (S-ZTPI) (Carelli, Wiberg & Wiberg, 2011) was administered in order to study the stability and change in BTP-level. The results showed a great stability in the BTP-level (Wiberg, Sircova, Wiberg& Carelli, in press), although a small change was observed. The 14 interviews were analyzed according to Interpretative Phenomenological Analysis (IPA). The result shows a consciousness about the "now" among the participants and a synchronicity between the present and the past and also between the present and the future. The results give strength to a holistic present scale (Zimbardo & Boyd, 2008) and an "extended now".
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| 5. |
- Wiberg, Britt, et al.
(författare)
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Balanced time perspective : developing empirical profile and exploring its stability over time
- 2017
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Ingår i: Time perspective. - London : Palgrave Macmillan. - 9781137601902 - 9781137601919 ; , s. 63-95
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Bokkapitel (refereegranskat)abstract
- Balanced time perspective (BTP) is characterized by flexible switching between a person's past, present and future time orientations, depending on situational demands, personal resources, experiences, and social evaluations. The present study aimed to explore the psychological characteristics of people with a BTP profile and attain a deeper understanding of the BTP construct. Seven people with BTP profiles were investigated using in-depth interviews, self-report instruments, and a projective test. By testing the participants on two occasions within an 18-month interval, we investigated the stability of BTP. Analyses showed that participants were aware of the "now" and had a synchronicity between the present and the past, and also between the present and the future. Results indicated a degree of temporal stability in the BTP profile and that people's interpretations and interactions within the surrounding context of events influences their time perspectives.
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| 6. |
- Wiberg, Marie, 1976-, et al.
(författare)
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Operationalizing balanced time perspective in a Swedish sample
- 2012
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Ingår i: The International Journal of Educational and Psychological Assessment. - : Time Taylor Academic Journals. - 2094-0734. ; 12:1, s. 95-107
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Tidskriftsartikel (refereegranskat)abstract
- Past research suggests that biases in temporal orientation may have systematic effects ongoal-directed behavior and that physical and mental well-being is associated with balanced time perspective (BTP, Zimbardo and Boyd, 1999). The aim of this study was to examine different operationalizations of the BTP, and to present an alternative proposal in which BTP is measured as a multidimensional, rather than a binary, construct. Two versions of the Zimbardo Time Perspective Inventory (ZTPI) were administered to a sample of 502 individuals. The proposed operationalization shows important advantages in terms of ecological validity of the multidimensional definition, sample independency and possibility to adjust for country differences. Our proposal may also have practical implications both for individuals and groups, e.g. in working teams, psychological counseling, psychotherapy and when recruiting personal forleading positions.
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| 7. |
- Åström, Elisabeth, 1982-, et al.
(författare)
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Insights into features of anxiety through multiple aspects of psychological time
- 2014
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Ingår i: Journal of Integrative Psychology and Therapeutics. - : Herbert Publications Ltd. - 2054-4723. ; 2
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Tidskriftsartikel (refereegranskat)abstract
- Background: It is well-recognized that emotions and emotional disorders may alter the experience of time. Yet relatively little is known about different aspects of psychological time in relation to anxiety. The purpose of the present study was to explore several aspects of temporal processing, including time perspective, prospective and retrospective time estimation, in persons with anxiety symptoms.Methods: A total of 110 individuals with varying degrees of anxiety participated in two studies. They were assigned to two groups (anxiety–control) based on their scores on anxiety measurements. Participants also completed an inventory of time perspective and several time estimation tasks which were analyzed on a group-level. Depressive symptoms were assessed and used as a covariate in the second study.Results: Anxiety was significantly associated with Past Negative and Future Negative time perspectives as measured by the Swedish Zimbardo Time Perspective Inventory (S-ZTPI), even when controlling for the effect of depressive symptoms. No other significant differences were found.Conclusion: Exploring time perspective in persons with anxious symptoms may provide important insights into features of anxiety. These findings may offer new ways of conceptualizing anxiety and provide suggestions for treatment strategies.
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| 9. |
- Brohlin, Maria, et al.
(författare)
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Aging effect on neurotrophic activity of human mesenchymal stem cells
- 2012
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Ingår i: PLOS ONE. - San Francisco : Public Library of Science. - 1932-6203. ; 7:9, s. e45052-
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Tidskriftsartikel (refereegranskat)abstract
- Clinical efficacy of stem cells for nerve repair is likely to be influenced by issues including donor age and in vitro expansion time. We isolated human mesenchymal stem cells (MSC) from bone marrow of young (16–18 years) and old (67–75 years) donors and analyzed their capacity to differentiate and promote neurite outgrowth from dorsal root ganglia (DRG) neurons. Treatment of MSC with growth factors (forskolin, basic fibroblast growth factor, platelet derived growth factor-AA and glial growth factor-2) induced protein expression of the glial cell marker S100 in cultures from young but not old donors. MSC expressed various neurotrophic factor mRNA transcripts. Growth factor treatment enhanced the levels of BDNF and VEGF transcripts with corresponding increases in protein release in both donor cell groups. MSC in co-culture with DRG neurons significantly enhanced total neurite length which, in the case of young but not old donors, was further potentiated by treatment of the MSC with the growth factors. Stem cells from young donors maintained their proliferation rate over a time course of 9 weeks whereas those from the old donors showed increased population doubling times. MSC from young donors, differentiated with growth factors after long-term culture, maintained their ability to enhance neurite outgrowth of DRG. Therefore, MSC isolated from young donors are likely to be a favourable cell source for nerve repair.
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| 10. |
- Brohlin, Maria, et al.
(författare)
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Characterisation of human mesenchymal stem cells following differentiation into Schwann cell-like cells
- 2009
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Ingår i: Neuroscience research. - : Elsevier BV. - 0168-0102 .- 1872-8111. ; 64:1, s. 41-49
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Tidskriftsartikel (refereegranskat)abstract
- Cell-based therapies provide a clinically applicable and available alternative to nerve autografts. Our previous studies have characterised rat-derived mesenchymal stem cells (MSC) and here we have investigated the phenotypic, molecular and functional characteristics of human-derived MSC (hMSC) differentiated along a Schwann cell lineage. The hMSC were isolated from healthy human donors and the identity of the undifferentiated hMSC was confirmed by the detection of MSC specific cells surface markers. The hMSC were differentiated along a glial cell lineage using an established cocktail of growth factors including glial growth factor-2. Following differentiation, the hMSC expressed the key Schwann cell (SC) markers at both the transcriptional and translational level. More importantly, we show the functional effect of hMSC on neurite outgrowth using an in vitro co-culture model system with rat-derived primary sensory neurons. The number of DRG sprouting neurites was significantly enhanced in the presence of differentiated hMSC; neurite length and density (branching) were also increased. These results provide evidence that hMSC can undergo molecular, morphological and functional changes to adopt a SC-like behaviour and, therefore, could be suitable as SC substitutes for nerve repair in clinical applications.
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