| 1. |
- Bryland, Anna, et al.
(författare)
-
Citrate treatment reduces endothelial death and inflammation under hyperglycaemic conditions.
- 2012
-
Ingår i: Diabetes & Vascular Disease Research. - : SAGE Publications. - 1752-8984 .- 1479-1641. ; 9:1, s. 42-51
-
Tidskriftsartikel (refereegranskat)abstract
- Hyperglycaemia and glucose degradation products (GDPs) are closely associated with oxidative stress and inflammation in diabetic patients, a condition that leads to endothelial dysfunction and cardiovascular problems. We evaluated the effect of citrate and gluconate on glucose- and GDP-induced endothelial inflammation by measuring changes in viability, inflammation and function in primary human umbilical vein endothelial cells (HUVECs). The extent of apoptosis/necrosis was measured by flow cytometry and visualised with confocal microscopy by staining with annexin V or propidium iodide, respectively. Protein kinase C-βII (PKC-βII) activation was evaluated with Western blotting. Incubation with glucose (30 mM) and GDP (50 µM) significantly increased PKC-βII expression, endothelial cell death and inflammation. The addition of citrate decreased hyperglycaemia-induced apoptosis (p = 0.021), necrosis (p = 0.04) and reduced PKC-βII expression (p = 0.021) down to background levels. Citrate improved endothelial function by reducing the inflammatory markers(p = 0.01) and by decreasing neutrophil diapedesis (p = 0.012). These results suggest that citrate may have therapeutic potential by reducing hyperglycaemia-induced endothelial inflammation and abolishing endothelial dysfunction.
|
|
| 2. |
- Bryland, Anna, et al.
(författare)
-
Infusion fluids contain harmful glucose degradation products.
- 2010
-
Ingår i: Intensive Care Medicine. - : Springer Science and Business Media LLC. - 0342-4642 .- 1432-1238. ; May 4, s. 1213-1220
-
Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: Glucose degradation products (GDPs) are precursors of advanced glycation end products (AGEs) that cause cellular damage and inflammation. We examined the content of GDPs in commercially available glucose-containing infusion fluids and investigated whether GDPs are found in patients' blood. METHODS: The content of GDPs was examined in infusion fluids by high-performance liquid chromatography (HPLC) analysis. To investigate whether GDPs also are found in patients, we included 11 patients who received glucose fluids (standard group) during and after their surgery and 11 control patients receiving buffered saline (control group). Blood samples were analyzed for GDP content and carboxymethyllysine (CML), as a measure of AGE formation. The influence of heat-sterilized fluids on cell viability and cell function upon infection was investigated. RESULTS: All investigated fluids contained high concentrations of GDPs, such as 3-deoxyglucosone (3-DG). Serum concentration of 3-DG increased rapidly by a factor of eight in patients receiving standard therapy. Serum CML levels increased significantly and showed linear correlation with the amount of infused 3-DG. There was no increase in serum 3-DG or CML concentrations in the control group. The concentration of GDPs in most of the tested fluids damaged neutrophils, reducing their cytokine secretion, and inhibited microbial killing. CONCLUSIONS: These findings indicate that normal standard fluid therapy involves unwanted infusion of GDPs. Reduction of the content of GDPs in commonly used infusion fluids may improve cell function, and possibly also organ function, in intensive-care patients.
|
|
| 3. |
|
|
| 4. |
- Nyström, Anna, et al.
(författare)
-
Anti-Müllerian hormone and fertility in women after childhood cancer treatment : Association with current infertility risk classifications
- 2024
-
Ingår i: PLoS ONE. - 1932-6203. ; 19:8, s. 1-18
-
Tidskriftsartikel (refereegranskat)abstract
- Background To identify childhood cancer survivors (CCSs) at risk of premature ovarian insufficiency (POI) and impaired fertility is important given its impact on quality of life. The aim of this study was to assess ovarian markers and fertility outcomes in adult female CCSs. We used the Swedish and the PanCareLIFE classifications for infertility risk grouping. Methods 167 CCSs, at median age 34.6 years (19.3–57.8) with a median follow-up time of 25.4 years (11.6–41.3), and 164 healthy matched controls were included in this cross-sectional study. We assessed anti-Müllerian hormone (AMH) levels, antral follicle count (AFC), ovarian volume (OV), and fertility outcomes. Based on gonadotoxic treatments given, CCSs were categorized into infertility risk groups. Results The median levels of AMH, AFC and OV were lower in CCSs (1.9 vs. 2.1 ng/ml, 12.0 vs. 13.0, 6.8 vs. 8.0 cm3) compared with controls, although statistically significant only for OV (p = 0.021). AMH levels in CCSs <40 years were lower for those classified as high-risk (p = 0.034) and very high-risk (p<0.001) for infertility, based on the Swedish risk classification. Similarly, AFC was reduced in the high-risk (p<0.001) and the very high-risk groups (p = 0.003). CCSs of all ages showed a trend towards impaired fertility, especially in the very high-risk group. POI was diagnosed in 22/167 CCSs, of whom 14 were in the high- and very high-risk groups. The results according to the PanCareLIFE classification were similar. Conclusion Both the Swedish and the PanCareLIFE infertility risk classifications are reliable tools for identifying those at risk of reduced ovarian markers and fertility, as well as POI. We recommend fertility preservation counselling for patients receiving highly gonadotoxic treatments (i.e., Cyclophosphamide Equivalent Dose ≥6 g/m2, radiotherapy exposure to ovaries or stem cell transplantation) with follow-up at a young reproductive age due to the risk of a shortened reproductive window.
|
|
| 5. |
- Wieslander, Anna
(författare)
-
“The Hultqvist doctrine”–Swedish security and defence policy after the Russian annexation of Crimea
- 2022
-
Ingår i: Defence Studies. - : Informa UK Limited. - 1470-2436 .- 1743-9698. ; 22:1, s. 35-59
-
Tidskriftsartikel (refereegranskat)abstract
- This article analyses how Sweden has adapted to the worsened security situation in its vicinity following Russia’s illegal annexation of Crimea and war in Eastern Ukraine. The paper builds on security policy research focused on neoclassic realism and small state strategies in order to explore how the, broadly unknown, security policy from 2009 has been implemented in practice since 2014, through what observers call the “Hultqvist doctrine.” The aim is to bring clarity to how the Hultqvist doctrine best can be understood–as a non-aligned or an integration policy?–and how the strategy relates to both systemic forces and domestic circumstances. The paper addresses the puzzle posed by structural realism, that the systemic forces would work to encourage Sweden to align in face of the threat that emerged in 2014. Still, Sweden has resisted NATO membership. To this end, I construct and apply an analytical framework that not only reveals the degree of novelty in the doctrine, but also allows for an evaluation of integration in three dimensions–openness, inclusiveness and comprehensiveness–in combination with a screening dimension. Following neoclassical realism, the study furthermore identifies domestic conditions that hinder policy flexibility.
|
|
| 6. |
- Wieslander, Anna
(författare)
-
What makes an ally? Sweden and Finland as NATO’s closest partners
- 2019
-
Ingår i: Journal of Transatlantic Studies. - : Springer Science and Business Media LLC. - 1479-4012 .- 1754-1018. ; 17:2, s. 194-222
-
Tidskriftsartikel (refereegranskat)abstract
- From 2013 onward, Sweden and Finland gradually distinguish themselves from all other NATO partners in order to meet the growing challenge how to defend the Baltics. In the analysis, the concept of ‘informal ally’, in contrast to ‘formal ally’, is introduced. A synthesized analytical framework is used to evaluate the process of ever closer partner cooperation and its shifting focus from cooperative security to collective defense. For NATO, the concept of informal allies is central to address. How do informal allies impact NATO in decision-making, operational planning and crisis response? How can NATO balance in solving its core tasks efficiently, without undermining itself as a multilateral institution?
|
|
