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- Agrell, Beata, et al.
(författare)
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Ett laglöst tillstånd
- 2012
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Annan publikation (populärvet., debatt m.m.)
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| 4. |
- Atienza-Párraga, Alba, et al.
(författare)
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Epigenomic re-configuration of primary multiple myeloma underlies the synergistic effect of combined DNMT and EZH2 inhibition.
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Multiple myeloma (MM) is characterized by an overexpression of EZH2 and a subsequent increase in H3K27me3-mediated silencing. However, the genome-wide redistribution of this mark in context with other epigenetic tags remains largely unexplored. Here, we show that EZH2 physically interacts with DNMT1 and that combined inhibition leads to a reduced G2/M arrest and increased apoptosis in MM. In addition, we present a catalogue of the genomic regulatory regions in normal plasma cells (NPC) as defined by their individual combination of histone marks. We used ChIP-seq and ATAC-seq data to generate whole-genome NPC chromatin annotations which we further analysed using DNA methylation arrays and RNA-seq. Comparison between NPC and MM demonstrated that, despite the global hypomethylation, enhancers show a tendency towards a higher DNA methylation levels in MM, whereas Polycomb and heterochromatic sites, highly methylated in NPC, show intermediate levels of the mark. Across all examined regulatory regions, 5-azacytidine treatment strongly reduced DNA methylation in MM. Furthermore, we find an extensive re-structuration of the global histone patterns in MM. We noticed a widespread increase in H3K27me3 except at active TSSs/promoters and enhancers, where we found a selective gain of the mark, suggestive of a directed silencing. In contrast, poised TSSs lose H3K27me3 and gain the activation mark H3K27ac, reflecting potential activation. Taken together, we present a comprehensive map of the epigenomic changes in MM as compared to NPC and provide insights into the interplay between EZH2 and DNMT1 in MM.
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| 8. |
- Hassila Karlsson, Carl Johan, et al.
(författare)
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Influence of scanning strategy on residual stresses in laser powder bed fusion manufactured alloy 718: Modeling and experiments
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- Residual stresses are a known phenomenon in additively manufactured materials. The residual stresses increase the risk of cracks, limit in-service performance, and distort printed parts. In this work, thermo-mechanical finite element models using the hatch-by-hatch and layer-by-layer approach, and the inherent strain method has been developed and applied to predict the effects of different scanning strategies on the deflection and the residual stresses of two PBF-LB processed geometries. To account for viscoplasticty and relaxation effects, a mechanism-based material model have been implemented and used. It is shown that the hatch-by-hatch approach and inherent strain method both successfully predicted the experimentally measured deflections of the first geometry, which was printed using different scanning directions. To predict the stress field experimentally, high-energy synchrotron measurements have been used to. The thermo-mechanical models and the inherent strain method both captures the trend of experimentally measured residual stress fields, although with an overall underprediction. The predictions of the models were evaluated, and their accuracy discussed in terms of physical aspects of the Powder Bed Fusion – Laser Beam process.
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| 10. |
- Kothapalli, Satya V.V.N. 1985-, et al.
(författare)
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Investigation of Polymer-Shelled Microbubble Motions in Acoustophoresis
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- The objective of this paper is to explore the trajectory motion of microsize (typically smaller than a redblood cell) encapsulated polymer-shelled gas bubbles propelled by radiation force in an acousticstanding-wave field and to compare the corresponding movements of solid polymer microbeads. Theexperimental setup consists of a microfluidic chip coupled to a piezoelectric crystal (PZT) with aresonance frequency of about 2.8 MHz. The microfluidic channel consists of a rectangular chamberwith a width, w, corresponding to one wavelength of the ultrasound standing wave. It creates one fullwave ultrasound of a standing-wave pattern with two pressure nodes at4w and43w and threeantinodes at 0,2w , and w. The peak-to-peak amplitude of the electrical potential over the PZT wasvaried between 1 and 10 volts. From Gor’kov’s potential equation, the acoustic contrast factor, Φ, forthe polymer-shelled microbubbles was calculated to about -60.7. Experimental results demonstratethat the polymer-shelled microbubbles are translated and accumulated at the pressure antinode planes.This trajectory motion of polymer-shelled microbubbles toward the pressure antinode plane is similarto what has been described for other acoustic contrast particles with a negative Φ. First, primaryradiation forces dragged the polymer-shelled microbubbles into proximity with each other at thepressure antinode planes. Then, secondary radiation forces caused them to aggregate at different spotsalong the channel. The relocation time for polymer-shelled microbubbles was 40 times shorter thanthat for polymer microbeads, and in contrast to polymer microbeads, the polymer-shelledmicrobubbles were actuated even at driving voltages (proportional to radiation forces) as low as 1 volt.In short, the polymer-shelled microbubbles demonstrate the behavior attributed to the negativeacoustic contrast factor particles and thus can be trapped at the antinode plane and thereby seperatedfrom solid particles, such as cells. This phenomenon could be utilized in exploring future applications,such as bioassay, bioaffinity, and cell interaction studies in vitro in a well-controlled environment.
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