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| 2. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 3. |
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- 2019
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Tidskriftsartikel (refereegranskat)
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| 4. |
- Kristan, Matej, et al.
(författare)
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The Sixth Visual Object Tracking VOT2018 Challenge Results
- 2019
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Ingår i: Computer Vision – ECCV 2018 Workshops. - Cham : Springer Publishing Company. - 9783030110086 - 9783030110093 ; , s. 3-53
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2018 is the sixth annual tracker benchmarking activity organized by the VOT initiative. Results of over eighty trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The evaluation included the standard VOT and other popular methodologies for short-term tracking analysis and a “real-time” experiment simulating a situation where a tracker processes images as if provided by a continuously running sensor. A long-term tracking subchallenge has been introduced to the set of standard VOT sub-challenges. The new subchallenge focuses on long-term tracking properties, namely coping with target disappearance and reappearance. A new dataset has been compiled and a performance evaluation methodology that focuses on long-term tracking capabilities has been adopted. The VOT toolkit has been updated to support both standard short-term and the new long-term tracking subchallenges. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website (http://votchallenge.net).
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| 5. |
- Xiong, Wei, et al.
(författare)
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Thermodynamic reassessment of the Cu-Mg-Ni system with brief comments on the thermodynamic modeling of the sub-systems
- 2008
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Ingår i: Calphad. - : Elsevier BV. - 0364-5916 .- 1873-2984. ; 32:4, s. 675-685
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Tidskriftsartikel (refereegranskat)abstract
- The Cu-Mg-Ni system was reassessed using the CALPHAD (CALculation of PHAse Diagram) approach, in order to provide a reliable thermodynamic description. A set of self-consistent thermodynamic parameters for the Cu-Mg-Ni system was obtained. The liquidus projection and the reaction scheme were generated by using the present thermodynamic parameters. Significant improvements have been made, compared with the previous assessments. Thermodynamic modeling for the three sub-systems was briefly reviewed. Further work is of interest to refine the thermodynamic description of the binary systems, in order to avoid the appearance of the artificial phase equilibria in liquid above 2500 degrees C.
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| 7. |
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| 8. |
- Zhang, Wei-Wei, et al.
(författare)
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Phase equilibria of the Fe-Ni-Si system at 850 degrees C
- 2009
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Ingår i: Journal of Alloys and Compounds. - : Elsevier BV. - 0925-8388 .- 1873-4669. ; 481:1-2, s. 509-514
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Tidskriftsartikel (refereegranskat)abstract
- By means of X-ray diffraction, metallography, scanning electron microscopy with energy dispersive Xray analysis, and electron probe microanalysis, the constitution of the ternary Fe-Ni-Si system at 850 degrees C over the entire composition range was established with 24 alloys. Ten of the three-phase equilibria were well determined at 850 degrees C. The existence of the ternary compound Fe5Ni3Si2 (tau(1)) with a cubic crystal structure at 850 degrees C is confirmed. The homogeneity ranges were measured and the lattice parameters were determined for most observed phases.
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| 9. |
- Zhang, Wei-Wei, et al.
(författare)
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Thermodynamic Assessment of the Cu-B System Supported by Key Experiment and First-Principles Calculations
- 2009
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Ingår i: Journal of Phase Equilibria and Diffusion. - : Springer Science and Business Media LLC. - 1547-7037 .- 1863-7345. ; 30:5, s. 480-486
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Tidskriftsartikel (refereegranskat)abstract
- The Cu-B system was investigated via a hybrid approach of key experiment and thermodynamic modeling. Based on the critically assessed Cu-B phase diagram, seven crucial alloys were selected and prepared by arc melting the pure elements. An inductively coupled plasma-atomic emission spectrometric analysis was conducted to determine the compositions of the prepared alloys. The phase equilibria were determined by using x-ray diffraction, electron probe microanalysis, and differential thermal analysis. The temperature associated with the eutectic reaction, L double left right arrow (B) + (Cu); was measured to be 1028 +/- 2 degrees C. First-principles calculations indicate that the energy of inserting a B atom into the interstitial vacancy (Va) site of the lattice for Cu atoms is marginally lower than that of substituting for a Cu atom with a B atom. Consequently, the sublattice model (Cu)(B, Va) in which B atoms occupy the interstitial sites was employed for the fcc (Cu) phase rather than the model (Cu, B)(Va) in which B atoms substitute for Cu atoms. A thermodynamic modeling of the Cu-B system was then performed by considering the reliable literature data and the present experimental results. A good agreement between modeling and experiment was obtained.
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| 10. |
- Ablikim, M., et al.
(författare)
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Branching fraction measurement of J/ψ→KSKL and search for J/ψ→KSKS
- 2017
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Ingår i: Physical Review D. - 2470-0010 .- 2470-0029. ; 96:11
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Tidskriftsartikel (refereegranskat)abstract
- Using a sample of 1.31 x 10(9) J/Psi events collected with the BESIII detector at the BEPCII collider, we study the decays of J/Psi -> KSKL and KSKS. The branching fraction of J/Psi -> KSKL is determined to be B(J/Psi -> KSKL) = (1.93 +/- 0.01 (stat) +/- 0.05 (syst)) x 10(-4), which significantly improves on previous measurements. No clear signal is observed for the J/Psi -> KSKS process, and the upper limit at the 95% confidence level for its branching fraction is determined to be B(J/Psi -> KSKS) < 1.4 x 10(-8), which improves on the previous searches by 2 orders in magnitude and reaches the order of the Einstein-Podolsky-Rosen expectation.
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