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- Kristan, M., et al.
(författare)
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The Eighth Visual Object Tracking VOT2020 Challenge Results
- 2020
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Ingår i: Computer Vision. - Cham : Springer International Publishing. - 9783030682378 ; , s. 547-601
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Konferensbidrag (refereegranskat)abstract
- The Visual Object Tracking challenge VOT2020 is the eighth annual tracker benchmarking activity organized by the VOT initiative. Results of 58 trackers are presented; many are state-of-the-art trackers published at major computer vision conferences or in journals in the recent years. The VOT2020 challenge was composed of five sub-challenges focusing on different tracking domains: (i) VOT-ST2020 challenge focused on short-term tracking in RGB, (ii) VOT-RT2020 challenge focused on “real-time” short-term tracking in RGB, (iii) VOT-LT2020 focused on long-term tracking namely coping with target disappearance and reappearance, (iv) VOT-RGBT2020 challenge focused on short-term tracking in RGB and thermal imagery and (v) VOT-RGBD2020 challenge focused on long-term tracking in RGB and depth imagery. Only the VOT-ST2020 datasets were refreshed. A significant novelty is introduction of a new VOT short-term tracking evaluation methodology, and introduction of segmentation ground truth in the VOT-ST2020 challenge – bounding boxes will no longer be used in the VOT-ST challenges. A new VOT Python toolkit that implements all these novelites was introduced. Performance of the tested trackers typically by far exceeds standard baselines. The source code for most of the trackers is publicly available from the VOT page. The dataset, the evaluation kit and the results are publicly available at the challenge website (http://votchallenge.net ).
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- Klionsky, Daniel J., et al.
(författare)
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Guidelines for the use and interpretation of assays for monitoring autophagy
- 2012
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Ingår i: Autophagy. - : Informa UK Limited. - 1554-8635 .- 1554-8627. ; 8:4, s. 445-544
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Forskningsöversikt (refereegranskat)abstract
- In 2008 we published the first set of guidelines for standardizing research in autophagy. Since then, research on this topic has continued to accelerate, and many new scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Accordingly, it is important to update these guidelines for monitoring autophagy in different organisms. Various reviews have described the range of assays that have been used for this purpose. Nevertheless, there continues to be confusion regarding acceptable methods to measure autophagy, especially in multicellular eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers or volume of autophagic elements (e.g., autophagosomes or autolysosomes) at any stage of the autophagic process vs. those that measure flux through the autophagy pathway (i.e., the complete process); thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from stimuli that result in increased autophagic activity, defined as increased autophagy induction coupled with increased delivery to, and degradation within, lysosomes (in most higher eukaryotes and some protists such as Dictyostelium) or the vacuole (in plants and fungi). In other words, it is especially important that investigators new to the field understand that the appearance of more autophagosomes does not necessarily equate with more autophagy. In fact, in many cases, autophagosomes accumulate because of a block in trafficking to lysosomes without a concomitant change in autophagosome biogenesis, whereas an increase in autolysosomes may reflect a reduction in degradative activity. Here, we present a set of guidelines for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes. These guidelines are not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to monitor autophagy. In these guidelines, we consider these various methods of assessing autophagy and what information can, or cannot, be obtained from them. Finally, by discussing the merits and limits of particular autophagy assays, we hope to encourage technical innovation in the field.
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- Dong, X.-P., et al.
(författare)
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The type IV mucolipidosis-associated protein TRPML1 is an endolysosomal iron release channel
- 2008
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Ingår i: Nature. - : Springer Science and Business Media LLC. - 0028-0836 .- 1476-4687. ; 455:7215, s. 992-996
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Tidskriftsartikel (refereegranskat)abstract
- TRPML1 (mucolipin 1, also known as MCOLN1) is predicted to be an intracellular late endosomal and lysosomal ion channel protein that belongs to the mucolipin subfamily of transient receptor potential (TRP) proteins. Mutations in the human TRPML1 gene cause mucolipidosis type IV disease (ML4). ML4 patients have motor impairment, mental retardation, retinal degeneration and iron-deficiency anaemia. Because aberrant iron metabolism may cause neural and retinal degeneration, it may be a primary cause of ML4 phenotypes. In most mammalian cells, release of iron from endosomes and lysosomes after iron uptake by endocytosis of Fe3+-bound transferrin receptors, or after lysosomal degradation of ferritin-iron complexes and autophagic ingestion of iron-containing macromolecules, is the chief source of cellular iron. The divalent metal transporter protein DMT1 (also known as SLC11A2) is the only endosomal Fe2+ transporter known at present and it is highly expressed in erythroid precursors. Genetic studies, however, suggest the existence of a DMT1-independent endosomal and lysosomal Fe2+ transport protein. By measuring radiolabelled iron uptake, by monitoring the levels of cytosolic and intralysosomal iron and by directly patch-clamping the late endosomal and lysosomal membrane, here we show that TRPML1 functions as a Fe2+ permeable channel in late endosomes and lysosomes. ML4 mutations are shown to impair the ability of TRPML1 to permeate Fe2+ at varying degrees, which correlate well with the disease severity. A comparison of TRPML1-/-ML4 and control human skin fibroblasts showed a reduction in cytosolic Fe2+ levels, an increase in intralysosomal Fe 2+ levels and an accumulation of lipofuscin-like molecules in TRPML1-/- cells. We propose that TRPML1 mediates a mechanism by which Fe2+ is released from late endosomes and lysosomes. Our results indicate that impaired iron transport may contribute to both haematological and degenerative symptoms of ML4 patients. ©2008 Macmillan Publishers Limited. All rights reserved.
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- Shen, X-G, et al.
(författare)
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Downregulation of caspase-9 is a frequent event in patients with stage II colorectal cancer and correlates with poor clinical outcome
- 2010
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Ingår i: COLORECTAL DISEASE. - : Blackwell Publishing Ltd. - 1462-8910. ; 12:12, s. 1213-1218
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Tidskriftsartikel (refereegranskat)abstract
- Objective To evaluate the clinical significance of caspase-9 mRNA expression and investigate its prognostic value in stage II colorectal cancer. Method Quantitative real-time RT-PCR was used to analyse caspase-9 mRNA expression in cancer tissue and corresponding normal mucosa from 120 patients. Results Compared with normal mucosa, the expression of caspase-9 mRNA was found to be downregulated in cancer tissue (P = 0.001). Poorly differentiated cancer showed lower mRNA expression than cancer with greater differentiation (P = 0.031). The Kaplan-Meier survival analysis demonstrated that patients with downregulated caspase-9 showed a worse overall survival (P = 0.012) and disease-free survival (P = 0.022). Coxs proportional hazards regression model confirmed that expression of caspase-9 was the strongest prognostic factor in stage II colorectal cancer. Conclusion The mRNA expression of caspase-9 can be used as an independent prognostic factor for patients with stage II colorectal cancer.
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- Feng, S. Z., et al.
(författare)
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Siloxane-Terminated Side Chain Engineering of Acceptor Polymers Leading to Over 7% Power Conversion Efficiencies in All-Polymer Solar Cells
- 2017
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Ingår i: ACS Macro Letters. - : American Chemical Society (ACS). - 2161-1653. ; 6:11, s. 1310-1314
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Tidskriftsartikel (refereegranskat)abstract
- To investigate the influence of functional pendent groups on acceptor polymers and photovoltaic properties of all-polymer solar cells (all-PSCs), two novel acceptor polymers containing siloxane-terminated side chains are synthesized and characterized. Increasing the content of siloxane-terminated side chains can reduce pi-pi stacking distance and improve crystalline behavior, yet lead to poorer solubility of the acceptor polymers. By modulating the proper loadings of siloxane-terminated side chains on the acceptor polymers, the PBDB-T:PNDI-Si25 all-PSC attains a maximal power conversion efficiency (PCE) of 7.4% with an outstanding fill factor of 0.68. The results provide, new insights for developing high-performance all-PSCs through functional group engineering on the acceptor polymers, to achieve good solubility, polymer miscibility, and blend morphology.
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- Fu, Y., et al.
(författare)
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Quantum effects and nanofabrications in scaling metal-oxide-semiconductor devices
- 2006. - 1
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Ingår i: Handbook of semiconductor nanostructures and nanodevices. - : American Scientific Publishers. - 1588830780 - 158883073X - 9781588830739 ; , s. 229-256
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Handbook of Semiconductor Nanostructures and Nanodevices is the World's first multivolume handbook covering a wide variety of advanced and emerging developments in the field of semiconductor nanotechnology. Semiconductor materials are the key elements of continued scientific and technological developments made in the fields of electronics, optoelectronics, photonics, and magnetic devices. The five-volume set is an unprecedented encyclopedic reference that covers growth and processing of semiconductor nanostructured materials by MBE, CVD, PVD, electrochemical, and other techniques, all types of III-V, IV, and II-VI semiconductor nanomaterials, nanofabrication by bottom-up and top-down approaches, precise control and uniformity of semiconductor self-assembled nanostructures, quantum dots, and quantum wells, nanowires, nanoclusters, nanocrystals, and nanoparticles, heterojunctions and interface properties, physical, and chemical properties, nanoscale spectroscopic characterization techniques, and applications of semiconductor devices in nanoelectronics, nano-optoelectronics, nanometrology, nanocircuits, nano-imprint lithography, heterojunction devices, laser diodes, LEDs, solar cells, terahertz devices, thermoelectric devices, NEMS/MEMS, optical switches, infrared detectors,, computers, wireless communications, magnetic random access memory (MRAM) and ferroelectric random access memory (FeRAM), spintronic and data storage devices, single-electron transistors, quantum computing devices, .etc. Both experimental and theoretical aspects of semiconductor nanoscience and nanotechnology are covered. It is a must-have handbook for university libraries, research establishments, government laboratories, and high-tech companies engaged in research and development of semiconductors. It is written for a broad range of audience with different backgrounds and educational level, compiling past two decades of pioneering research. It provides in-depth information to research professiona
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| 10. |
- Gao, Feng, et al.
(författare)
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Effects of substrate temperature on Bi0.8La0.2FeO3 thin films prepared by pulsed laser deposition
- 2007
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Ingår i: Thin Solid Films. - : Elsevier. - 0040-6090 .- 1879-2731. ; 515:13, s. 5366-5373
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Tidskriftsartikel (refereegranskat)abstract
- Bi0.8La0.2FeO3 thin films on Pt/TiO2/SiO2/Si substrates at various substrate temperatures from 500 degrees C to 750 degrees C are prepared by pulsed laser deposition, and their microstructures and ferroelectric/magnetic properties are carefully investigated using various techniques. It is observed that the crystallographic orientation and Fe-ion valence state depend significantly on the substrate temperature, which consequently influences considerably on the ferroelectric and magnetic properties of the thin films. A considerable improvement of the ferroelectric and magnetic properties of the thin films can be achieved by optimizing the substrate temperature for deposition. (C) 2007 Elsevier B.V. All rights reserved.
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