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Träfflista för sökning "WFRF:(Yadegari Farnaz) "

Sökning: WFRF:(Yadegari Farnaz)

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1.
  • Beech, Jason P., et al. (författare)
  • Sample preparation for single-cell whole chromosome analysis
  • 2012
  • Ingår i: Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. - : Chemical and Biological Microsystems Society. - 9780979806452 ; , s. 998-999
  • Konferensbidrag (refereegranskat)abstract
    • In this work we present an integrated system for whole chromosome analysis of single bacterium. Using whole genome barcoding techniques, which offer direct and rapid microscopic visualization of the entire genome in one field-of-view, we aim to rapidly identify individual bacterium. We are developing our device to achieve the crucial, and difficult process of isolating a bacterium, removing the DNA in one piece and transferring it to a nano-channel for visualisation. In order to achieve control over the bacteria we encapsulate them in agarose, using flow focusing. The encapsulated bacteria can then be transported in microchannels to proximity with the nanochannels and then chemically lysis can be performed. Following lysis the intact genome can be extracted and transferred to the meandering nanochannel for analysis. We believe this device holds the potential to significantly decrease analysis times for single cell, whole genome analysis with the potential of opening up for automated, high-throughput genome analysis in microfluidic systems.
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2.
  • Yadegari, Farnaz, et al. (författare)
  • Microfluidic connections to hollow nanowires
  • 2011
  • Ingår i: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. - 9781618395955 ; 1, s. 386-388
  • Konferensbidrag (refereegranskat)abstract
    • We have fabricated a device in which hollow nanowires (HNWs) lying on a glass surface are connected to microfluidic channels for the purpose of guiding DNA and to characterize the fluidic properties of our HNWs. The dimensions of our HNWs (inner diameter 10-80 nm, outer diameter 50-200 nm and length several μm) can be made on a scale much smaller than the size of a cell and on the same order of magnitude as the Debye length, making them interesting both for applications involving direct chemical access to the cytosol of living cells and for investigations of basic transport phenomena.
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