| 1. |
- Chen, Gefei, et al.
(författare)
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Full-Length Minor Ampullate Spidroin Gene Sequence
- 2012
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Ingår i: PLOS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 7:12, s. e52293-
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Tidskriftsartikel (refereegranskat)abstract
- Spider silk includes seven protein based fibers and glue-like substances produced by glands in the spider's abdomen. Minor ampullate silk is used to make the auxiliary spiral of the orb-web and also for wrapping prey, has a high tensile strength and does not supercontract in water. So far, only partial cDNA sequences have been obtained for minor ampullate spidroins (MiSps). Here we describe the first MiSp full-length gene sequence from the spider species Araneus ventricosus, using a multidimensional PCR approach. Comparative analysis of the sequence reveals regulatory elements, as well as unique spidroin gene and protein architecture including the presence of an unusually large intron. The spliced full-length transcript of MiSp gene is 5440 bp in size and encodes 1766 amino acid residues organized into conserved nonrepetitive N- and C-terminal domains and a central predominantly repetitive region composed of four units that are iterated in a non regular manner. The repeats are more conserved within A. ventricosus MiSp than compared to repeats from homologous proteins, and are interrupted by two nonrepetitive spacer regions, which have 100% identity even at the nucleotide level.
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| 2. |
- Liu, Dan, et al.
(författare)
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Using InSAR to identify hydrological connectivity and barriers in a highly fragmented wetland
- 2020
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Ingår i: Hydrological Processes. - : Wiley. - 0885-6087 .- 1099-1085. ; 34:23, s. 4417-4430
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Tidskriftsartikel (refereegranskat)abstract
- Hydrological connectivity is a critical determinant of wetland functions and health, especially in wetlands that have been heavily fragmented and regulated by human activities. However, investigating hydrological connectivity in these wetlands is challenging due to the costs of high-resolution and large-scale monitoring required in order to identify hydrological barriers within the wetlands. To overcome this challenge, we here propose an interferometric synthetic aperture radar (InSAR)-based methodology to map hydrologic connectivity and identify hydrological barriers in fragmented wetlands. This methodology was applied along 70 transects across the Baiyangdian, the largest freshwater wetland in northern China, using Sentinel 1A and 1B data, covering the period 2016-2019. We generated 58 interferograms providing information on relative water level changes across the transects that showed the high coherence needed for the assessment of hydrological connectivity. We mapped the permanent and conditional (temporary) barriers affecting connectivity. In total, 11% of all transects are permanently disconnected by hydrological barriers across all interferograms and 58% of the transects are conditionally disconnected. Areas covered by reed grasslands show the most undisturbed hydrological connectivity while some of these barriers are the result of ditches and channels within the wetland and low water levels during different periods of the year. This study highlights the potential of the application of Wetland InSAR to determine hydrological connectivity and location of hydrological barriers in highly fragmented wetlands, and facilitates the study of hydrological processes from large spatial scales and long-time scales using remote sensing technique.
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| 3. |
- Zhao, Yunlong, et al.
(författare)
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Stable Alkali Metal Ion Intercalation Compounds as Optimized Metal Oxide Nanowire Cathodes for Lithium Batteries
- 2015
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Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 15:3, s. 2180-2185
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Tidskriftsartikel (refereegranskat)abstract
- Intercalation of ions in electrode materials has been explored to improve the rate capability in lithium batteries and supercapacitors, due to the enhanced diffusion of Li+ or electrolyte cations. Here, we describe a synergistic effect between crystal structure and intercalated ion by experimental characterization and ab initio calculations, based on more than 20 nanomaterials: five typical cathode materials together with their alkali metal ion intercalation compounds A-M-O (A = Li, Na, K, Rb; M = V, Mo, Co, Mn, Fe-P). Our focus on nanowires is motivated by general enhancements afforded by nanoscale structures that better sustain lattice distortions associated with charge/discharge cycles. We show that preintercalation of alkali metal ions in V-O and Mo-O yields substantial improvement in the Li ion charge/discharge cycling and rate, compared to A-Co-O, A-Mn-O, and A-Fe-P-O. Diffraction and modeling studies reveal that preintercalation with K and Rb ions yields a more stable interlayer expansion, which prevents destructive collapse of layers and allow Li ions to diffuse more freely. This study demonstrates that appropriate alkali metal ion intercalation in admissible structure can overcome the limitation of cyclability as well as rate capability of cathode materials, besides, the preintercalation strategy provides an effective method to enlarge diffusion channel at the technical level, and more generally, it suggests that the optimized design of stable intercalation compounds could lead to substantial improvements for applications in energy storage.
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