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Sökning: WFRF:(Zhang Chi) > Bokkapitel

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1.
  • Zhang, Chi, et al. (författare)
  • Cells in Microfluidics
  • 2011
  • Ingår i: MICROFLUIDICS. - Berlin, Heidelberg : Springer Berlin/Heidelberg. - 9783642230493 ; , s. 295-321
  • Bokkapitel (refereegranskat)abstract
    • Microfluidic devices offer a realistic environment for cell cultures as it is related to scales found in biological systems. The aim is to create more in vivo like systems, in comparison to 2D plate cultures. Creating 3D cell culture constructs increase the cell's functionality. By controlling the microenvironment (e.g., cell matrix, flow rate, temperature) cell functionality can be increased even more. As microfluidic devices allow for precise control of the microenvironment, they are a paramount tool to study stem cells and their differentiation caused by external factors. We will give an overview of the use of microfluidic devices for some biological problems, and especially as a cell culture platforms. We focus on 3D cell cultures and stem cells and their microenvironment.
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2.
  • Zhang, Chi (författare)
  • Perfusion culture of mammalian cells in a microfluidic channel with a built-in pillar array
  • 2012
  • Ingår i: Single-Cell Analysis. - Totowa, NJ : Springer Science+Business Media B.V.. - 9781617795664 ; , s. 83-94
  • Bokkapitel (refereegranskat)abstract
    • In vitro culture of mammalian cells is fundamental to various biological studies such as single cell analysis, pathological research, and drug/therapy development. However, there are limitations with the current in vitro cell culture methods. Cells tend to lose their specific functions due to the lack of a cellular microenvironment when they are maintained under standard culture conditions. Microscale devices can be a novel tool to reestablish a cellular microenvironment for culturing mammalian cells in vitro and maintaining their differentiated functions. Different microscale cell culture techniques have been developed to suit different biological applications. This chapter describes a method to trap and culture mammalian cells in a microfluidic channel with a built-in pillar array.
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refereegranskat (2)
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Zhang, Chi (2)
van Noort, Danny (1)
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