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Sökning: WFRF:(Zhu ZH) > Lunds universitet

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  • Chen, ZH, et al. (författare)
  • Regulation of MUC5AC mucin secretion by depletion of AQP5 in SPC-A1 cells
  • 2006
  • Ingår i: Biochemical and Biophysical Research Communications. - : Elsevier BV. - 1090-2104 .- 0006-291X. ; 342:3, s. 775-781
  • Tidskriftsartikel (refereegranskat)abstract
    • Airway mucus is regulated by many inflammatory mediators such as ILs, TNF-alpha, EGF, PGF2 alpha, LT, and so on. Recently, the relationship between membrane ion channel and Mucus production has been under investigation. The present study aimed to examine whether AQP5 was involved in modulation of mucin expression and secretion in airway submucosal gland cells (SPC-Al). A recombinant plasmid (pShAQP5) containing small hairpin RNA expression cassette targeting AQP5 sequence was constructed. In pShAQP5 transiently transfected cells, ELISA showed MUC5AC synthesis and secretion were increased by 57.9% and 85.3%, respectively, on day 5 after pShAQP5 transfection. While in five stably transfected clones (shAQP5-G1, G2, G3, A2, and A5), the upregulated levels of M MAC mRNA were 118%, 165%, 65% 123%, and 38%, respectively. The elevated levels of M MAC synthesis and secretion varied from 59-156% and 33-166%, respectively. This is the first reliable investigation of the regulation of MUC5AC mucin secretion by silencing AQP5. Further study of the regulatory mechanism between AQPs and mucins may provide new strategies for development of novel antihypersecretory drugs in airway diseases.
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  • Xiong, Y, et al. (författare)
  • Removal of formic acid from wastewater using three-phase three-dimensional electrode reactor
  • 2003
  • Ingår i: Water, Air and Soil Pollution. - 1573-2932. ; 144:1, s. 67-79
  • Tidskriftsartikel (refereegranskat)abstract
    • A new electrochemical reactor, three-phase three-dimensional electrode reactor, was designed and used to investigate the removal of formic acid from simulated wastewater. The experimental results were assessed in term of Chemical Oxygen Demand (COD) removal efficiency. The results showed that the three-phase three-dimensional electrodes could effectively remove formic acid. Its COD removal efficiency was much higher than those of two-dimensional electrodes and common three-dimensional electrodes, respectively. The COD removal efficiency of the three-phase three-dimensional electrodes using air as a sparge gas increased 13.5% relative to that using nitrogen as a sparge gas. The observation indicated that the sparged air in the three-phase three-dimensional electrodes not only participated in some physical processes but also played an important role in electrochemical reactions to assist COD removal.
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  • Resultat 1-4 av 4

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