Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Hammarström Per) "

Sökning: WFRF:(Hammarström Per)

  • Resultat 1-10 av 210
Sortera/gruppera träfflistan
  • Niklasson, Mia, et al. (författare)
  • Membrane-Depolarizing Channel Blockers Induce Selective Glioma Cell Death by Impairing Nutrient Transport and Unfolded Protein/Amino Acid Responses
  • 2017
  • Ingår i: Cancer Research. - 0008-5472 .- 1538-7445. ; 77:7, s. 1741-1752
  • Tidskriftsartikel (refereegranskat)abstract
    • Glioma-initiating cells (GIC) are considered the underlying cause of recurrences of aggressive glioblastomas, replenishing the tumor population and undermining the efficacy of conventional chemotherapy. Here we report the discovery that inhibiting T-type voltage-gated Ca(2+) and KCa channels can effectively induce selective cell death of GIC and increase host survival in an orthotopic mouse model of human glioma. At present, the precise cellular pathways affected by the drugs affecting these channels are unknown. However, using cell-based assays and integrated proteomics, phosphoproteomics, and transcriptomics analyses, we identified the downstream signaling events these drugs affect. Changes in plasma membrane depolarization and elevated intracellular Na(+), which compromised Na(+)-dependent nutrient transport, were documented. Deficits in nutrient deficit acted in turn to trigger the unfolded protein response and the amino acid response, leading ultimately to nutrient starvation and GIC cell death. Our results suggest new therapeutic targets to attack aggressive gliomas.
  • Nyström, Sofie, et al. (författare)
  • Seed-dependent templating of murine AA amyloidosis
  • 2017
  • Ingår i: Amyloid : Journal of Protein Folding Disorders. - Taylor & Francis. - 1350-6129. ; 24:sup1, s. 140-141
  • Tidskriftsartikel (övrigt vetenskapligt)
  • Sjölander, Daniel, et al. (författare)
  • Establishing the fluorescent amyloid ligand h-FTAA for studying human tissues with systemic and localized amyloid
  • 2016
  • Ingår i: Amyloid : Journal of Protein Folding Disorders. - 1350-6129 .- 1744-2818. ; 23:2, s. 98-108
  • Tidskriftsartikel (refereegranskat)abstract
    • Rapid and accurate detection of amyloid deposits in routine surgical pathology settings are of great importance. The use of fluorescence microscopy in combination with appropriate amyloid specific dyes is very promising in this regard. Here we report that a luminescent conjugated oligothiophene, h-FTAA, rapidly and with high sensitivity and selectivity detects amyloid deposits in verified clinical samples from systemic amyloidosis patients with AA, AL and ATTR types; as well as in tissues laden with localized amyloidosis of AANF, AIAPP and ASem1 type. The probe h-FTAA emitted yellow red fluorescence on binding to amyloid deposits, whereas no apparent staining was observed in surrounding tissue. The only functional structure stained with h-FTAA showing the amyloidotypic fluorescence spectrum was Paneth cell granules in intestine. Screening of 114 amyloid containing tissues derived from 107 verified (Congo red birefringence and/or immunohistochemistry) amyloidosis patients revealed complete correlation between h-FTAA and Congo red fluorescence (107/107, 100% sensitivity). The majority of Congo red negative control cases (27 of 32, 85% specificity) were negative with h-FTAA. Small Congo red negative aggregates in kidney, liver, pancreas and duodenum were found by h-FTAA fluorescence in five control patients aged 72-83 years suffering from diverse diseases. The clinical significance of these false-positive lesions is currently not known. Because h-FTAA fluorescence is one magnitude brighter than Congo red and as the staining is performed four magnitudes lower than the concentration of dye, we believe that these inclusions are beyond detection by Congo red. We conclude that h-FTAA is a fluorescent hypersensitive, rapid and powerful tool for identifying amyloid deposits in tissue sections. Use of h-FTAA can be exploited as a rapid complementary technique for accurate detection of amyloid in routine surgical pathology settings. Our results also implicate the potential of the technique for detection of prodromal amyloidosis as well as for discovery of new amyloid-like protein aggregates in humans.
  • Forsberg, Göte, et al. (författare)
  • Presence of bacteria and innate immunity of intestinal epithelium in childhood celiac disease
  • 2004
  • Ingår i: American Journal of Gastroenterology. - 0002-9270. ; 99:5, s. 894-904
  • Tidskriftsartikel (refereegranskat)abstract
    • OBJECTIVES: Exposure to gliadin and related prolamins and appropriate HLA-DQ haplotype are necessary but not sufficient for contracting celiac disease (CD). Aberrant innate immune reactions could be contributing risk factors. Therefore, jejunal biopsies were screened for bacteria and the innate immune status of the epithelium investigated.METHODS: Children with untreated, treated, challenged CD, and controls were analyzed. Bacteria were identified by scanning electron microscopy. Glycocalyx composition and mucin and antimicrobial peptide production were studied by quantitative RT-PCR, antibody and lectin immunohistochemistry.RESULTS: Rod-shaped bacteria were frequently associated with the mucosa of CD patients, with both active and inactive disease, but not with controls. The lectin Ulex europaeus agglutinin I (UEAI) stained goblet cells in the mucosa of all CD patients but not of controls. The lectin peanut agglutinin (PNA) stained glycocalyx of controls but not of CD patients. mRNA levels of mucin-2 (MUC2), alpha-defensins HD-5 and HD-6, and lysozyme were significantly increased in active CD and returned to normal in treated CD. Their expression levels correlated to the interferon-gamma mRNA levels in intraepithelial lymphocytes. MUC2, HD-5, and lysozyme proteins were seen in absorptive epithelial cells. beta-defensins hBD-1 and hBD-2, carcinoembryonic antigen (CEA), CEA cell adhesion molecule-1a (CEACAM1a), and MUC3 were not affected.CONCLUSIONS: Unique carbohydrate structures of the glycocalyx/mucous layer are likely discriminating features of CD patients. These glycosylation differences could facilitate bacterial adhesion. Ectopic production of MUC2, HD-5, and lysozyme in active CD is compatible with goblet and Paneth cell metaplasia induced by high interferon-gamma production by intraepithelial lymphocytes.
  • Hedberg, Maria E, 1959-, et al. (författare)
  • Lachnoanaerobaculum a new genus in Lachnospiraceae; characterization of Lachnoanaerobaculum umeaense gen. nov., sp. nov., isolated from human small intestine, Lachnoanaerobaculum orale gen. nov., sp. nov., isolated from saliva and reclassification of Eubacterium saburreum (Prevot) Holdeman and Moore 1970 as Lachnoanaerobaculum saburreum comb. nov.
  • 2012
  • Ingår i: International Journal of Systematic and Evolutionary Microbiology. - 1466-5026. ; 62:11, s. 2685-2690
  • Tidskriftsartikel (refereegranskat)abstract
    • Two new obligately anaerobic Gram-positive, saccharolytic and non-proteolytic spore-forming bacilli (strain CD3:22 and N1) are described. Strain CD3:22 was isolated from a biopsy of the small intestine of a child with celiac disease and strain N1 from the saliva of a healthy young man. The cells of both strains were observed to be filamentous with lengths of approximately 5 to >20 µm, some of them curving and with swellings. The novel organisms produced H2S, NH3, butyric acid and acetic acid as major metabolic end products. Phylogenetic analyses, based on comparative 16S rRNA gene sequencing, revealed close relationships (98 % sequence similarity) between the two isolates, as well as the type strain of Eubacterium saburreum CCUG 28089T and four other Lachnospiraceae bacterium/E. saburreum-like organisms. This group of bacteria were clearly different from any of the 19 known genera in the family Lachnospiraceae. While Eubacterium spp. are reported to be non-spore-forming, reanalysis of E. saburreum CCUG 28089T confirmed that the bacterium, indeed, is able to form spores. Based on 16S rRNA gene sequencing, phenotypic and biochemical properties, CD3:22 (CCUG 58757T) and N1 (CCUG 60305T) represent new species of a new and distinct genus, named Lachnoanaerobaculum, in the family Lachnospiraceae [within the order Clostridiales, class Clostridia, phylum Firmicutes]. Strain CD3:22 is the type strain of the type species, Lachnoanaerobaculum umeaense gen. nov., sp. nov., of the proposed new genus. Strain N1 is the type strain of the species, Lachnoanaerobaculum orale gen. nov., sp. nov. Moreover, E. saburreum CCUG 28089T is reclassified as Lachnoanaerobaculum saburreum comb. nov.
  • Mincheva-Nilsson, Lucia, et al. (författare)
  • Human milk contains proteins that stimulate and suppress T lymphocyte proliferation.
  • 1990
  • Ingår i: Clinical and Experimental Immunology. - 0009-9104. ; 79:3, s. 463-9
  • Tidskriftsartikel (refereegranskat)abstract
    • The modulatory effect of human milk proteins from colostrum and late milk on the proliferative response of human T lymphocytes activated by mitogens (OKT3 and leucoagglutinin from Phaseolus vulgaris) and alloantigens was studied. High concentrations (10-100 micrograms/ml) of crude colostral milk proteins had an inhibitory effect on T cell growth while low concentrations (0.1-1 microgram/ml) enhanced T cells growth. In contrast, proteins from late milk did not inhibit T lymphocyte proliferation while the enhancing effect was retained. Colostrum was fractionated by ammonium sulphate precipitation and gel filtration on sepharose 6B. The inhibitory activity was recovered in a protein fraction containing lactoferrin as its major component. Lactoferrin was, however, not responsible for the observed inhibition. On the contrary, lactoferrin in most cases augmented the proliferative response induced by polyclonal activators. The inhibitory activity was found to bind concanavalin A-sepharose suggesting an association with glycoprotein. Inhibitory fractions contained glycoproteins of the following molecular sizes 26, 74/76 (doublet), 84, 145 and 160 kD under reducing conditions. The inhibitory effect appeared to be lymphocyte specific since the active fraction did not inhibit the growth of tissue culture cells (HeLa cells and human fibroblasts) or bacteria. Furthermore, the fraction was not toxic for lymphocytes. The inhibitory colostrum factor may prevent the newborn from overreacting immunologically against the environmental antigens encountered at birth.
Skapa referenser, mejla, bekava och länka
  • Resultat 1-10 av 210
fritt online (40)
Typ av publikation
tidskriftsartikel (158)
konferensbidrag (20)
annan publikation (15)
bokkapitel (6)
rapport (6)
forskningsöversikt (3)
visa fler...
doktorsavhandling (1)
recension (1)
visa färre...
Typ av innehåll
refereegranskat (151)
övrigt vetenskapligt (46)
populärvet., debatt m.m. (13)
Hammarström, Per, (113)
Nilsson, Peter, (48)
Hammarström, Anne, (35)
Gustafsson, Per E., (29)
Nyström, Sofie, (22)
Hammarström, Per, 19 ... (21)
visa fler...
Lindgren, Mikael (21)
Carlsson, Uno, (20)
Hammarström, Per, 19 ... (17)
Jonsson, Bengt-Haral ... (12)
Nilsson, K. Peter R. ... (12)
San Sebastian, Migue ... (11)
Sjölander, Daniel, (10)
Åslund, Andreas, (10)
Carlsson, Uno, 1946- ... (9)
Konradsson, Peter, (9)
Virtanen, Pekka, (9)
Klingstedt, Therese, (9)
Lindgren, M (8)
Jucker, Mathias, (7)
Moparthi, Satish Bab ... (7)
Inganäs, Olle, (6)
Westermark, Gunilla ... (6)
Westermark, Per, (6)
Henriksson, Per, (6)
Janlert, Urban, (6)
Villebeck, Laila, (6)
Jonsson, Bengt-Haral ... (6)
Herland, Anna, (6)
Hammarström, Ulf, (6)
Mason, Jeffrey, (6)
Sörgjerd, Karin, 197 ... (6)
Kågedal, Katarina, (5)
Wennberg, Patrik, (5)
Theorell, Töres (5)
Thor, Stefan, (5)
Westerlund, Hugo, (5)
Mårtensson, Lars-Gör ... (5)
Wennberg, Maria, (5)
Hammarström, Leif, (4)
Nilsson, Lars, (4)
Hammarström, Marie-L ... (4)
Kelly, J. W., (4)
Prokop, Stefan, (4)
Hammarström, Sten, (4)
Aguzzi, Adriano, (4)
Gustafsson, Per, 198 ... (4)
Johansson, Leif B. G ... (4)
Mishra, Rajesh, (4)
Simon, Rozalyn, (4)
visa färre...
Linköpings universitet (128)
Uppsala universitet (47)
Umeå universitet (43)
Mittuniversitetet (17)
Stockholms universitet (11)
Göteborgs universitet (7)
visa fler...
Karolinska Institutet (7)
VTI - Statens väg- och transportforskningsinstitut (6)
Lunds universitet (5)
Kungliga Tekniska Högskolan (2)
RISE (2)
Linnéuniversitetet (1)
Högskolan i Skövde (1)
visa färre...
Engelska (172)
Svenska (25)
Forskningsämne (UKÄ/SCB)
Naturvetenskap (79)
Medicin och hälsovetenskap (73)
Teknik (42)
Humaniora (20)
Samhällsvetenskap (4)


pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy