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1.
  • Jönsson, Mikael, et al. (författare)
  • Glacial clay affects foraging performance in a Patagonian fish and cladoceran
  • 2011
  • Ingår i: Hydrobiologia. - Springer. - 0018-8158. ; 663:1, s. 101-108
  • Tidskriftsartikel (refereegranskat)abstract
    • Climate change is altering temperatures and precipitation patterns all over the world. In Patagonia, Argentina, predicted increase in precipitation together with rapidly melting glaciers increase the surface runoff, and thereby the transport of suspended solids to recipient lakes. Suspended solids affect the visual conditions in the water which in turn restricts visual foraging. The native fish Aplochiton zebra Jenyns, and its filter-feeding cladoceran prey, Daphnia commutata Ekman, were subjected to foraging experiments at three turbidity levels. A. zebra foraging rate was substantially reduced at naturally occurring turbidity levels and the filtering rate of D. commutata was reduced at the highest turbidity level. This indicates that Daphnia may be partly released from predation from A. zebra at the same time as it can maintain relatively high feeding rates as turbidity increases. Lower foraging rates at the same time as the metabolic demand increases, through increased temperatures, may result in larger effects on A. zebra than could be expected from increases in turbidity or temperature alone. Turbidity may, as an indirect effect of climate change, decrease planktivore foraging rates and thereby alter the interaction strength between trophic levels.
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2.
  • Moubah, Reda, et al. (författare)
  • Soft Room-Temperature Ferromagnetism of Carbon-Implanted Amorphous Fe93Zr7 Films
  • 2013
  • Ingår i: APPL PHYS EXPRESS. - 1882-0778. ; 6:5, s. 053001
  • Tidskriftsartikel (refereegranskat)abstract
    • We report on the effect of carbon implantation on the structural, electronic, and magnetic properties of Fe93Zr7 (FeZr) amorphous films. Extended X-ray absorption fine structure measurements on (FeZr)(100-x)C-x (x = 0, 5.5, and 11) indicate the incorporation of carbon in the FeZr matrix, with an increase of the Fe-Fe distance by implanting carbon. X-ray photoelectron spectroscopy measurements reveal the creation of Fe-C bonds after implantation. A significant enhancement of the Curie temperature and decrease of the coercivity are observed in the carbon-implanted films. Moreover, the non collinear ferromagnetism of the as-grown FeZr film diminishes upon carbon implantation.
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3.
  • Salomonsson, Stina, et al. (författare)
  • A Population-based Investigation of the Autoantibody Profile in Mothers of Children with Atrioventricular Block
  • 2011
  • Ingår i: Scandinavian Journal of Immunology. - Blackwell Publishing. - 0300-9475. ; 74:5, s. 511-517
  • Tidskriftsartikel (refereegranskat)abstract
    • The objective of the study was to investigate the antigen specificity and occurrence of individual autoantibodies in mothers of children diagnosed with atrioventricular (AV) block in a nation-wide setting. Patients with AV block detected before 15 years of age were identified using national quality registries as well as a network of pediatric and adult cardiologists and rheumatologists at the six university hospitals in Sweden. Patients with gross heart malformations, surgically or infectiously induced blocks were excluded. Blood samples were obtained from the mothers and maternal autoantibody profile, including the occurrence of antibodies against Ro52, Ro60, La, SmB, SmD, RNP-70k, RNP-A, RNP-C, CENP-C, Scl-70, Jo-1, ribosomal RNP and histones was investigated in 193 mothers of children with AV block by immunoblotting and ELISA. Autoantibody reactivity was detected in 48% (93/193) of the mothers of children with AV block. In autoantibody-positive mothers, the vast majority, 95% (88/93), had antibodies against Ro52, while 63% (59/93) had autoantibodies to Ro60 and 58% (54/93) had autoantibodies to La. In addition, 13% (12/93) of the autoantibody-positive mothers had antibodies to other investigated antigens besides Ro52, Ro60 and La, and of these anti-histone antibodies were most commonly represented, detected in 8% (7/93) of the mothers. In conclusion, this Swedish population-based study confirms that maternal autoantibodies may associate with heart block in the child. Further, our data demonstrate a dominant role of Ro52 antibodies in association with AV block.
4.
  • Ahlqvist-Rastad, Jane, et al. (författare)
  • Erythropoietin therapy and cancer related anaemia: updated Swedish recommendations
  • 2007
  • Ingår i: Medical Oncology. - Humana Press. - 1357-0560. ; 24:3, s. 267-272
  • Tidskriftsartikel (refereegranskat)abstract
    • Due to concerns related to treatment with erythropoietin (EPO) and possible negative effects on tumour control, a workshop was organised by the Medical Products Agency of Sweden with the aim to revise national treatment guidelines if needed. In patients with solid tumours, conflicting results have been reported with respect to tumour control and survival. Until further notice it is therefore recommended that EPO should be used restrictively in the treatment of patients with cancer and that the anticipated improvement in quality of life should be evaluated against potential risks.
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7.
  • Andersson, Henrik, et al. (författare)
  • Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells
  • 2010
  • Ingår i: JOURNAL OF BIOTECHNOLOGY. - Elsevier Science B.V., Amsterdam.. - 0168-1656. ; 150:1, s. 175-181
  • Tidskriftsartikel (refereegranskat)abstract
    • Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound. doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.
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8.
  • Asp, Julia, 1973-, et al. (författare)
  • Cardiomyocyte clusters derived from human embryonic stem cells share similarities with human heart tissue.
  • 2010
  • Ingår i: Journal of molecular cell biology. - 1759-4685. ; 2:5, s. 276-83
  • Tidskriftsartikel (refereegranskat)abstract
    • Cardiotoxicity testing is a key activity in the pharmaceutical industry in order to detect detrimental effects of new drugs. A reliable human in vitro model would both be beneficial in selection of lead compounds and be important for reducing animal experimentation. However, the human heart is a complex organ composed of many distinct types of cardiomyocytes, but cardiomyocyte clusters (CMCs) derived from human embryonic stem cells could be an option for a cellular model. Data on functional properties of CMCs demonstrate similarities to their in vivo analogues in human. However, development of an in vitro model requires a more thorough comparison of CMCs to human heart tissue. Therefore, we directly compared individually isolated CMCs to human fetal, neonatal, adult atrial and ventricular heart tissues. Real-time qPCR analysis of mRNA levels and protein staining of ion channels and cardiac markers showed in general a similar expression pattern in CMCs and human heart. Moreover, a significant decrease in beat frequency was noted after addition of Zatebradine, a blocker to I(f) involved in regulation of spontaneous contraction in CMCs. The results underscore the similarities of CMCs to human cardiac tissue, and further support establishment of novel cardiotoxicity assays based on the CMCs in drug discovery.
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9.
  • Bredberg, Anders, et al. (författare)
  • Clinical-scale generation of strongly CD83-expressing dendritic cells using extracorporeal photopheresis
  • 2007
  • Ingår i: Photodermatology, Photoimmunology and Photomedicine. - Blackwell Publishing. - 0905-4383. ; 23:4, s. 113-119
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: Many strategies are currently being pursued in order to generate mature dendritic cells (DC) to be used for immunotherapy. A potent anti-tumour influence by extracorporeal photopheresis has been documented for cutaneous T-cell lymphoma, and a major mechanism of action has been suggested to be generation of DC presenting tumour antigens. Purpose: To determine the potential of a simple clinical photopheresis protocol for large-scale development of mature DC. Methods: A standard monocyte-enriched leukapheresis preparation of 10(9)-10(10) cells was derived during each of five consecutive treatment sessions of a patient with cutaneous T-cell lymphoma. The cells were incubated overnight in autologous plasma with no addition of growth medium. Cell surface lymphocyte, monocyte and DC markers were determined using multi-colour flow cytometry. Results: We find signs of activation of the CD14+ monocytes, as well as the appearance of a minor population of mature DC negative for CD14 but with strong CD83 expression. Conclusions: With a procedure appropriate for routine clinical use, a total number of 10(6)-10(7) DC ready for patient reinfusion can be prepared within 24 h. Our findings indicate the need to further explore the capacity of photopheresis to stimulate cancer patients' anti-tumour defence reaction.
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