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Sökning: WFRF:(Jonsson Anders) > Göteborgs universitet > Refereegranskat

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  • Andersson, Henrik, et al. (författare)
  • Assaying cardiac biomarkers for toxicity testing using biosensing and cardiomyocytes derived from human embryonic stem cells
  • 2010
  • Ingår i: JOURNAL OF BIOTECHNOLOGY. - Elsevier Science B.V., Amsterdam.. - 0168-1656. ; 150:1, s. 175-181
  • Tidskriftsartikel (refereegranskat)abstract
    • Human embryonic stem cell (hESC) derived cardiomyocytes are in the present study being used for testing drug-induced cardiotoxicity in a biosensor set-up. The design of an in vitro testing alternative provides a novel opportunity to surpass previous methods based on rodent cells or cell lines due to its significantly higher toxicological relevance. In this report we demonstrate how hESC-derived cardiomyocytes release detectable levels of two clinically decisive cardiac biomarkers, cardiac troponin T and fatty acid binding protein 3, when the cardiac cells are exposed to the well-known cardioactive drug compound. doxorubicin. The release is monitored by the immuno-biosensor technique surface plasmon resonance, particularly appropriate due to its capacity for parallel and high-throughput analysis in complex media.
  • Asp, Julia, 1973-, et al. (författare)
  • Cardiomyocyte clusters derived from human embryonic stem cells share similarities with human heart tissue.
  • 2010
  • Ingår i: Journal of molecular cell biology. - 1759-4685. ; 2:5, s. 276-83
  • Tidskriftsartikel (refereegranskat)abstract
    • Cardiotoxicity testing is a key activity in the pharmaceutical industry in order to detect detrimental effects of new drugs. A reliable human in vitro model would both be beneficial in selection of lead compounds and be important for reducing animal experimentation. However, the human heart is a complex organ composed of many distinct types of cardiomyocytes, but cardiomyocyte clusters (CMCs) derived from human embryonic stem cells could be an option for a cellular model. Data on functional properties of CMCs demonstrate similarities to their in vivo analogues in human. However, development of an in vitro model requires a more thorough comparison of CMCs to human heart tissue. Therefore, we directly compared individually isolated CMCs to human fetal, neonatal, adult atrial and ventricular heart tissues. Real-time qPCR analysis of mRNA levels and protein staining of ion channels and cardiac markers showed in general a similar expression pattern in CMCs and human heart. Moreover, a significant decrease in beat frequency was noted after addition of Zatebradine, a blocker to I(f) involved in regulation of spontaneous contraction in CMCs. The results underscore the similarities of CMCs to human cardiac tissue, and further support establishment of novel cardiotoxicity assays based on the CMCs in drug discovery.
  • Bräutigam, Marcus, 1968-, et al. (författare)
  • Development of Swedish winter oat with gene technology and molecular breeding
  • 2006
  • Ingår i: J. Seed Science. - 0039-6990. ; 116:1-2, s. 12-35
  • Tidskriftsartikel (refereegranskat)abstract
    • In Sweden, oat (Avena sativa) is only grown as a spring crop. A Swedish winter oat, on the other hand, would give increased yields and would secure oat in Swedish agriculture. During three consecutive winters we performed field trials with oat aiming at identifying potential winter material. More than 300 varieties, originating from breeding programs all over the world, were tested. Plants were rated according to winter survival, vigour and general performance during the following growth season and more than 20 lines were identified that were cold hardier than present commercial oat varieties. In parallel experiments a cDNA library was constructed from cold induced English winter oat (Gerald) and ca 10000 EST sequences were generated. After data mining a UniGene set of 2800 oat genes was obtained. By detailed analysis of microarray data from cold stressed Arabidopsis and by advanced bioinformatics, gene interactions in the complex cold induced signal transduction pathway were deduced. By comparison to the oat UniGene set, several genes potentially involved in the regulation of cold hardiness in oat were identified. An Agrobacterium mediated transformation protocol was developed for one oat genotype. Key regulatory genes in cold acclimation will be introduced to oat by genetic transformation or modified by TILLING. Such genes will be used as molecular markers in intogression of winter hardiness to commercial oat.
  • Granhag, Pär-Anders, et al. (författare)
  • Deception among pairs: "Let's say we had lunch and hope they will swallow it!"
  • 2003
  • Ingår i: Psychology, Crime and Law. - Taylor & Francis. - 1477-2744. ; 9:2, s. 109-124
  • Tidskriftsartikel (refereegranskat)abstract
    • Deception research has neglected the fact that legal-workers often have to try to detect deceit on the basis of statements derived from pairs of suspects, each having been interrogated repeatedly. To remedy this shortcoming we conducted a study where each member of 10 truth-telling pairs and 10 lying pairs was interrogated twice about an alibi. One hundred and twenty undergraduate students were enrolled as lie-catchers. The main findings were that (a) overall deception detection accuracy was modest; (b) lie-catchers given access to a large number of statements did not outperform lie-catchers given access to a lesser number of statements; (c) when asked to justify their veracity assessments the most frequently reported cue was 'consistency within pairs of suspects'; (d) all cues to deception were of low diagnostic value. Psycho-legal aspects of integrating sequential information in deception detection contexts are discussed.
  • Jonsson, Marianne, 1962-, et al. (författare)
  • Novel 3D culture system with similarities to the human heart for studies of the cardiac stem cell niche.
  • 2010
  • Ingår i: Regenerative medicine. - 1746-076X. ; 5:5, s. 725-36
  • Tidskriftsartikel (refereegranskat)abstract
    • AIMS: The aim of this study was to develop a 3D culture system with similarities to the human heart, which was suitable for studies of adult cardiac stem or progenitor cells. MATERIALS & METHODS: Dissociated cells from human cardiac biopsies were placed in high-density pellet cultures and cultured for up to 6 weeks. Gene and protein expressions, analyzed by quantitative real-time PCR and immunohistochemistry, and morphology were studied in early and late pellets. RESULTS: Cells cultured in the 3D model showed similarities to human cardiac tissue. Moreover, markers for cardiac stem and progenitor cells were also detected after 6 weeks of culture, in addition to markers for signaling pathways active in stem cell niche regulation. CONCLUSIONS: The described 3D culture model could be a valuable tool when studying the influence of different compounds on proliferation and differentiation processes in cardiac stem or progenitor cells in cardiac regenerative research.
  • Jonsson, Sverrir Adalsteinn, et al. (författare)
  • The drumlin field and the geomorphology of the Mulajokull surge-type glacier, central Iceland
  • 2014
  • Ingår i: Geomorphology. - Elsevier. - 0169-555X. ; 207, s. 213-220
  • Tidskriftsartikel (refereegranskat)abstract
    • Here we present a new geomorphological map of the active drumlin field and the forefield of Millajokull, a surgetype outlet glacier, Iceland. The map is based on aerial photographs taken in 1995 and LiDAR data recorded in 2008. Mapping was done using ArcGIS 10 software on orthorectified imagery, LiDAR data and digital elevation models. The mapped landforms were initially identified on the aerial imagery and LiDAR and then groundchecked in the field. We mapped subglacial, supraglacial, ice-marginal, periglacial, and glaciofluvial landforms. The geomorphology of the Millajokull forefield is similar to that of the forefields of other surge-type glaciers in Iceland: with a highly streamlined forefield, crevasse-fill ridges, and series of glaciotectonic end moraines. However, the large number (i.e., 110) of drumlins forming the drumlin field is unique for modem Icelandic surge-type glaciers and, as yet, unique for contemporary glaciers in general. Also apparent is that the drumlins are wider and shorter in the distal part of the drumlin field and narrower and longer in the proximal part. Hence, the mapping reveals a development of the drumlins toward a more streamlined shape of the proximal landforms that have experienced more surges. The drumlins in the drumlin field are active, i.e., they form during the modern surges of MillajOkull. (C) 2013 Elsevier B.V. All rights reserved.
  • Sandstedt, Joakim, et al. (författare)
  • C-kit+ CD45- cells found in the adult human heart represent a population of endothelial progenitor cells.
  • 2010
  • Ingår i: Basic research in cardiology. - 1435-1803. ; 105:4, s. 545-56
  • Tidskriftsartikel (refereegranskat)abstract
    • Although numerous reports support the existence of stem cells in the adult heart, few studies have been conducted using human cardiac tissue. Therefore, cells from human cardiac atrial biopsies were analyzed regarding progenitor properties. Expression of stem cell markers was analyzed using fluorescence-activated cell sorting. This identified a small population of C-kit+ cells, which could be further subdivided based on expression of CD45. The C-kit+ CD45+ population was determined to be of mast cell identity, while the C-kit+ CD45- population expressed mRNA of the endothelial lineage. Since the number of cells obtainable from biopsies was limited, a comparison between directly isolated and monolayer and explant cultured cells, respectively, was carried out. While both cultures retained a small population of mast cells, only monolayer culture produced a stable and relatively high percentage of C-kit+ CD45- cells. This population was found to co-express endothelial progenitor cell markers such as CD31, CD34, CXCR4, and FLK-1. The mRNA expression profile was similar to the one from directly isolated cells. When sorted cells were cultured in endothelial differentiation medium, the C-kit+ CD45- population retained its expression of endothelial markers to a large extent, but downregulated progenitor markers, indicating further differentiation into endothelial cells. We have confirmed that the human cardiac atrium contains a small C-kit+ CD45- population expressing markers commonly found on endothelial progenitor cells. The existence of an endothelial progenitor population within the heart might have future implications for developing methods of inducing neovascularization after myocardial infarction.
  • Sandstedt, Joakim, et al. (författare)
  • Human C-kit+CD45- cardiac stem cells are heterogeneous and display both cardiac and endothelial commitment by single-cell qPCR analysis.
  • 2014
  • Ingår i: Biochemical and biophysical research communications. - 1090-2104. ; 443:1
  • Tidskriftsartikel (refereegranskat)abstract
    • C-kit expressing cardiac stem cells have been described as multipotent. We have previously identified human cardiac C-kit+CD45- cells, but only found evidence of endothelial commitment. A small cardiac committed subpopulation within the C-kit+CD45- population might however be present. To investigate this at single-cell level, right and left atrial biopsies were dissociated and analyzed by FACS. Only right atrial biopsies contained a clearly distinguishable C-kit+CD45- population, which was single-cell sorted for qPCR. A minor portion of the sorted cells (1.1%) expressed early cardiac gene NKX2.5 while most of the cells (81%) expressed late endothelial gene VWF. VWF- cells were analyzed for a wider panel of genes. One group of these cells expressed endothelial genes (FLK-1, CD31) while another group expressed late cardiac genes (TNNT2, ACTC1). In conclusion, human C-kit+CD45- cells were predominantly localized to the right atrium. While most of these cells expressed endothelial genes, a minor portion expressed cardiac genes.
  • Sandstedt, Joakim, et al. (författare)
  • Left atrium of the human adult heart contains a population of side population cells.
  • 2012
  • Ingår i: Basic research in cardiology. - 1435-1803. ; 107:2
  • Tidskriftsartikel (refereegranskat)abstract
    • Cardiac "side population" (SP) cells have previously been found to differentiate into both endothelial cells and cardiomyocytes in mice and rats, but there are no data on SP cells in the human adult heart. Therefore, human cardiac atrial biopsies were dissociated, stained for SP cells and analyzed with FACS. Identified cell populations were analyzed for gene expression by quantitative real-time PCR and subjected to in vitro differentiation. Only biopsies from the left atrium contained a clearly distinguishable population of SP cells (0.22 ± 0.08%). The SP population was reduced by co-incubation with MDR1 inhibitor Verapamil, while the ABCG2 inhibitor FTC failed to decrease the number of SP cells. When the gene expression was analyzed, SP cells were found to express significantly more MDR1 than non-SP cells. For ABCG2, there was no detectable difference. SP cells also expressed more of the stem cell-associated markers C-KIT and OCT-4 than non-SP cells. On the other hand, no significant difference in the expression of endothelial and cardiac genes could be detected. SP cells were further subdivided based on CD45 expression. The CD45-SP population showed evidence of endothelial commitment at gene expression level. In conclusion, the results show that a SP population of cells is present also in the human adult heart.
  • Sandstedt, Joakim, et al. (författare)
  • SSEA-4+ CD34- Cells in the Adult Human Heart Show the Molecular Characteristics of a Novel Cardiomyocyte Progenitor Population.
  • 2014
  • Ingår i: Cells, tissues, organs. - 1422-6421. ; 199:2-3, s. 103-116
  • Tidskriftsartikel (refereegranskat)abstract
    • Stage-specific embryonic antigen (SSEA) expression is used to describe the differentiation state of an embryonic stem cell (ESC). In human ESCs, SSEA-3 and SSEA-4 are highly expressed in undifferentiated cells and downregulated upon differentiation. SSEA-4 has also been described as a marker for adult stem cells in various tissues, including human neonatal cardiac tissue. However, there is currently little data on the expression of SSEAs in human adult cardiac tissue. We obtained right and left atrial biopsies from patients undergoing cardiac surgery. These were dissociated, stained for SSEAs and other cardiac stem cell markers and analyzed by flow cytometry. Directly isolated cells expressed variable levels of SSEA-1, SSEA-3 and SSEA-4. The SSEA-1+ population was established as contaminating hematopoietic cells. The SSEA-4+ population, on the other hand, could be subdivided based on the endothelial progenitor marker CD34. The SSEA-4+ CD34- population in the right atrium had a high gene expression of both early (TBX5, NKX2.5) and late (TNNT2) cardiomyocyte markers. The SSEA-4+ CD34+ population, on the other hand, overlapped with previously described C-kit+ CD45- cardiac stem cells. Primary monolayer-cultured cells retained expression of SSEAs while the cardiomyogenic specification in the SSEA-4+ CD34- population was lost. In tissue sections, SSEA-4+ cells could be identified both within and outside the myocardium. Within the myocardium, some SSEA-4+ cells coexpressed cardiomyogenic markers. In conclusion, the results show that the adult human heart expresses SSEAs and that there is a subpopulation of SSEA-4+ CD34- cells that show features of a cardiomyocyte progenitor population. © 2014 S. Karger AG, Basel.
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