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Sökning: WFRF:(Ottosson Mikael)

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  • Fagerberg, Linn, et al. (författare)
  • Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics
  • 2014
  • Ingår i: Molecular & Cellular Proteomics. - 1535-9476. ; 13:2, s. 397-406
  • Tidskriftsartikel (refereegranskat)abstract
    • Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody- based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to 80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.
  • Fallqvist, M., et al. (författare)
  • Nucleation and growth of CVD alpha-Al2O3 on TixOy template
  • 2012
  • Ingår i: Surface & Coatings Technology. - 0257-8972. ; 207, s. 254-261
  • Tidskriftsartikel (refereegranskat)abstract
    • The microstructure, phase and chemical composition of TixOy, templates used to nucleate alpha-Al2O3 on Ti(C,N) coated cemented carbide have been elucidated using scanning electron microscopy, X-ray diffraction, Auger electron spectroscopy and Time-of-Flight Secondary Ion Mass Spectrometry. Further, the adhesive strength of the alpha-Al2O3-TixOy-Ti(C,N) interfaces was investigated using scratch adhesion testing. The present study confirmed that the as-deposited template consisted of a Ti4O7 phase which during subsequent deposition of the Al2O3 layer transformed to a Ti3O5 phase and that the grown Al2O3 layer consisted of 100% alpha-Al2O3. Furthermore, the results showed that the lowest interfacial strength within the multilayer structure was exhibited by the Ti(C,N)-TixOy interface and that the transformation of Ti4O7 to Ti3O5 in the template resulted in formation of pores in the Ti(C,N)-template interface lowering the interfacial strength even more. The use of surface analysis techniques such as Auger electron spectroscopy and especially Time-of-Flight Secondary Ion Mass Spectrometry enabled trace element analyses using depth profiling to characterise the thin interfacial layers in detail. (c) 2012 Elsevier B.V. All rights reserved.
  • Tende, Talatu, et al. (författare)
  • Individual Identification and Genetic Variation of Lions (Panthera leo) from Two Protected Areas in Nigeria.
  • 2014
  • Ingår i: PLOS One. - Public Library of Science. - 1932-6203. ; 9:1, s. e84288
  • Tidskriftsartikel (refereegranskat)abstract
    • This survey was conducted in two protected areas in Nigeria to genetically identify individual lions and to determine the genetic variation within and between the populations. We used faecal sample DNA, a non-invasive alternative to the risky and laborious task of taking samples directly from the animals, often preceded by catching and immobilization. Data collection in Yankari Game Reserve (YGR) spanned through a period of five years (2008 -2012), whereas data in Kainji Lake National Park (KLNP) was gathered for a period of three years (2009, 2010 and 2012). We identified a minimum of eight individuals (2 males, 3 females, 3 unknown) from YGR and a minimum of ten individuals (7 males, 3 females) from KLNP. The two populations were found to be genetically distinct as shown by the relatively high fixation index (FST = 0.17) with each population exhibiting signs of inbreeding (YGR FIS = 0.49, KLNP FIS = 0.38). The genetic differentiation between the Yankari and Kainji lions is assumed to result from large spatial geographic distance and physical barriers reducing gene flow between these two remaining wild lion populations in Nigeria. To mitigate the probable inbreeding depression in the lion populations within Nigeria it might be important to transfer lions between parks or reserves or to reintroduce lions from the zoos back to the wild.
  • Adner, Mikael, et al. (författare)
  • Contractile endothelin-B (ETB) receptors in human small bronchi
  • 1996
  • Ingår i: European Respiratory Journal. - ERS Journals Ltd. - 0903-1936 .- 1399-3003. ; 9:2, s. 351-355
  • Tidskriftsartikel (refereegranskat)abstract
    • Endothelins (ETs) are a family of novel regulatory peptides and various lines of evidence suggest an important role for ETs in regulating pulmonary function. Two receptors for endothelin, ETA and ETB, have been found in the human lung, and according to recent studies a non-ETA receptor seems to mediate the contraction of large sized human bronchi. Several studies have emphasized the importance of small bronchi in the pathogenesis of airway disease. In the present paper, improved methodology was used which enables in vitro studies of small human bronchi down to a diameter of 0.5-1.0 mm. Using the new methodology we have tried to further characterize this receptor. Small bronchi from the distal parts of the bronchial tree were obtained from pulmonary tissue removed from 15 patients with lung cancer. They were dissected and cut into ring segments, in which isometric tension was recorded. ET-1, ET-2 and ET-3 elicited strong concentration-dependent contractions of the human small bronchus. Basically, the three peptides were equipotent with about the same maximal response. Upon reapplication, they all showed the same tachyphylaxis pattern, reaching half the initial contraction. Comparative analysis of IRL 1620, a selective ETB receptor agonist, revealed that the effect of the ETB agonist was, in all respects, similar to the responses induced by the ETs. PD 145065, a combined ETA/ETB receptor antagonist competitively inhibited the contractions induced by IRL 1620, whereas FR139317, a selective ETA receptor antagonist, was without effect. In conclusion, the present study shows that accurate measurements can be made in vitro on small human bronchi and all present data are in favour of an ETB receptor mediating endothelin-induced contraction of human bronchi smaller than 1.0 mm.
  • Albin, Maria, et al. (författare)
  • Risk Assessment for Carbon Nanotubes
  • 2011
  • Ingår i: Arbete och Hälsa. - Occupational and Environmental Medicine, Sahlgrenska Academy, University of Gothenburg. - 0346-7821. - 978-91-85971-32-9 ; 45:5, s. 1
  • Annan publikation (refereegranskat)
  • Backlund, Sandra, 1986-, et al. (författare)
  • Energy efficiency potentials and energy management practices in Swedish firms
  • 2012
  • Konferensbidrag (övrigt vetenskapligt)abstract
    • In order to improve energy efficiency and reach the EU:s 20-20-20 primary energy saving target, focus has mainly been on diffusion of technology. Previous studies have illustrated large untapped energy saving potentials from implementing energy management practices in firms. Energy management practices have large effects on energy utilization and also a short pay-back time. According to these studies, energy management practices also effect investment decisions and the outcome of investments in energy efficient technologies. This paper investigates to what extent energy management practices influence firms estimation of energy efficiency potentials. Further it investigates two Swedish policy programs that promote industrial energy management practices: The Programme For improving Energy efficiency in energy-intensive industry (PFE) and the energy audit program and whether these have increased energy management practices in Swedish firms. A multiple case study has been conducted in order to investigate energy practices in firms in different industrial sectors. Employment of energy management varies between firms. The firms estimate equal energy efficiency potentials from implementation of energy efficient technology as for energy management practices. In total the firms estimate energy efficiency potentials of 12 %. The study shows that firms that have participated in the programs work more actively with energy management. This can be illustrated by the fact that 75 % of the firms that have not participated in any of the programs lack a person responsible for energy management and 50 % also lack a long term energy strategy. For firms that have participated in the programs the corresponding figures are 30 % and 33 %. The results indicate an untapped potential of energy efficiency measures that could be reached through increased energy management in Swedish industries.
  • Backlund, Sandra, 1986-, et al. (författare)
  • Extending the Energy efficiency gap
  • 2012
  • Ingår i: Energy Policy. - Elsevier. - 0301-4215. ; 51, s. 392-396
  • Tidskriftsartikel (refereegranskat)abstract
    • In order to reach the EU: s 20–20–20 primary energy savings target, energy efficiency needs to increase. Previous research on energy use and energy efficiency has focused mainly on the diffusion of energy efficient technologies. The discrepancy between optimal and actual implementation of energy efficient technologies has been illustrated in numerous articles and is often referred to as the energy efficiency gap. However, efficient technologies are not the only ways to increase energy efficiency. Empirical studies have found that a cost-effective way to improve energy efficiency is to combine investments in energy-efficient technologies with continuous energy management practices. By including energy management into an estimated energy efficiency potential this paper introduces an extended energy efficiency gap, mainly in manufacturing industries and the commercial sector. The inclusion of energy management components in future energy policy will play an important role if the energy savings targets for 2020, and later 2050, are to be met in the EU.
  • Behrenz, Lars, et al. (författare)
  • Arbetsgivares perspektiv på subventionerade anställningar En kartläggning
  • 2015
  • Rapport (övrigt vetenskapligt)abstract
    • Trots en kraftig ökning av antalet subventionerade anställningar från mitten av 2000-talet är det ett stort antal arbetsgivare som inte använder sig av lönesubvent-ioner. Denna rapport har försökt att kartlägga och analysera arbetsgivares per-spektiv på lönekostnadssubventioner. När det gäller analyser som berör de arbets-givare som använt sig av lönesubventioner är den viktigaste förklaringsfaktorn att man använt sig av subventioner tidigare. Våra intervjuer visar också att de arbets-givare som använder sig av lönesubventioner ofta har ett socialt ansvarstagande. Ett av huvudresultaten med följande studie är att 95 procent av de som använder sig av subventioner är nöjda. Omfattningen i pengar och personens kvalifikationer var mest avgörande för anställningsbeslutet. Av de som inte använt sig av löne-subventioner är det 20 procent som inte känt till att möjligheten finns. Intervjuer med arbetsgivare utan erfarenhet av att anställa med lönesubventioner visar att de är skeptiska att hitta lämplig personal genom att anställa med lönesubventioner. När vi ställer en hypotetisk fråga till samtliga arbetsgivare om vad som skulle kunna få dem att anställa med en subvention framkommer möjligheten att få pröva aktuell person som den klart viktigaste faktorn. Utifrån våra resultat föreslår vi bl.a. att Arbetsförmedlingen, i ett försöksupplägg, ska ges möjlighet att agera bemanningsföretag och vi föreslår också att man ska genomföra en omfattande informationskampanj till arbetsgivare.
  • Bergström, Per, 1981-, et al. (författare)
  • Automatic in-line inspection of shape based on photogrammetry
  • 2016
  • Ingår i: SPS16. - Lund : SPS16.
  • Konferensbidrag (refereegranskat)abstract
    • We are describing a fully automatic in-line shape inspection system for controlling the shape of moving objects on a conveyor belt. The shapes of the objects are measured using a full-field optical shape measurement method based on photogrammetry. The photogrammetry system consists of four cameras, a flash, and a triggering device. When an object to be measured arrives at a given position relative to the system, the flash and cameras are synchronously triggered to capture images of the moving object. From the captured images a point-cloud representing the measured shape is created. The point-cloud is then aligned to a CAD-model, which defines the nominal shape of the measured object, using a best-fit method and a feature-based alignment method. Deviations between the point-cloud and the CAD-model are computed giving the output of the inspection process. The computational time to create a point-cloud from the captured images is about 30 seconds and the computational time for the comparison with the CAD-model is about ten milliseconds. We report on recent progress with the shape inspection system.
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