SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Ryde Ulf) ;pers:(Olsen L.)"

Sökning: WFRF:(Ryde Ulf) > Olsen L.

  • Resultat 1-2 av 2
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Olsen, L, et al. (författare)
  • Binding of benzylpenicillin to metallo-beta-lactamase: A QM/MM study
  • 2004
  • Ingår i: Journal of Physical Chemistry: B. - American Chemical Society. - 1520-6106. ; 108:45, s. 17639-17648
  • Tidskriftsartikel (refereegranskat)abstract
    • Metallo-beta-lactamases are bacterial enzymes that may function with either one or two zinc ions bound in the active site. In this work, the binding of benzylpenicillin to mono-zinc metallo-beta-lactamase from Bacillus cereus has been investigated in a docking procedure applying a combined quantum mechanical/molecular mechanical method as the final step. It is demonstrated that the substrate can bind with the carbonyl oxygen of the lactam ring coordinating to the zinc ion, and with the zinc-bound hydroxide ion in position for a nucleophilic attack on the carbonyl carbon of the lactam ring. In some structures, both the histidine and the cysteine at the other (unoccupied) metal-binding site are in a proper position to function as proton shuttles in proton transfer from the previously zinc-bound hydroxide, to the nitrogen in the lactam ring. In addition, the hydrophobic region formed by Phe34, Val39, Trp59, and Ala89 interacts with the phenyl group of benzylpenicillin, whereas the carboxylate group may be stabilized by Lys171 and Asn180. Alternatively, the carboxylate can bind to the zinc ion, prohibiting the nucleophilic attack of the zinc-bound hydroxide on the lactam carbonyl carbon. However, such a structure is energetically disfavored compared to the other enzyme-substrate complexes.
  •  
2.
  • Olsen, L, et al. (författare)
  • Lactam hydrolysis catalyzed by mononuclear metallo-beta-lactamases: A density functional study
  • 2003
  • Ingår i: Journal of Physical Chemistry B. - American Chemical Society. - 1520-6106. ; 107:10, s. 2366-2375
  • Tidskriftsartikel (refereegranskat)abstract
    • Two central steps in the hydrolysis of lactam antibiotics catalyzed by mononuclear metallo--lactamases, formation of the tetrahedral intermediate and its breakdown by proton transfer, are studied for model systems using the density functional B3LYP method. Metallo--lactamases have two metal ion binding sites, one of which is occupied in the mononuclear species. In this work it is assumed that catalysis takes place at zinc site 1, which is modeled by the metal ion, three imidazole rings, and a hydroxide ion. The lactam ring, a minimal model of -lactam antibiotics, is initially coordinating to the zinc ion. Potential proton shuttles from the second (unoccupied) metal-binding site (water, Asp, or Cys) are included in some calculations. The calculated reaction barrier for formation of the tetrahedral intermediate is 13 kcal/mol, close to what is observed experimentally for the rate-limiting step. The barrier for the breakdown of the intermediate is low, 0-10 kcal/mol, if it is assisted by a water molecule or by a Cys or Asp model. Thus, the results indicate that proton transfer is not rate-limiting, and that any of the residues from the second metal site may function as proton shuttle. For most studied systems, the tetrahedral structure is a stable intermediate. Moreover, the C-N bond in the lactam ring is intact in this intermediate, as well as in the following transition state-its cleavage is induced by proton transfer to the nitrogen atom in the lactam ring. However, for the model with Asp as a proton shuttle, attack of the zinc-bond hydroxide ion seems to be concerted with the proton transfer. We have also studied the effect of replacing one of the histidine ligands by an asparagine or glutamine residue, giving a zinc site representative of other subclasses of metallo--lactamases. This has only a small effect on the calculated reaction barriers. Likewise, if the zinc ion is replaced by cadmium, only small changes in the reaction barrier for proton transfer are seen, whereas the barrier for the formation of the tetrahedral intermediate increases by 3 kcal/mol and the intermediate is destabilized by 5 kcal/mol.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-2 av 2
Typ av publikation
tidskriftsartikel (2)
Typ av innehåll
refereegranskat (2)
Författare/redaktör
Ryde, Ulf (2)
Hemmingsen, L. (2)
Rasmussen, Torben (1)
Antony, J (1)
Adolph, H-W (1)
Lärosäte
Lunds universitet (2)
Språk
Engelska (2)

År

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy