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Sökning: WFRF:(Ali Mina) > (2020)

  • Resultat 1-3 av 3
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1.
  • Abbafati, Cristiana, et al. (författare)
  • 2020
  • Tidskriftsartikel (refereegranskat)
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2.
  • Beal, Jacob, et al. (författare)
  • Robust estimation of bacterial cell count from optical density
  • 2020
  • Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
  • Tidskriftsartikel (refereegranskat)abstract
    • Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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3.
  • Ghanbarikarekani, Mina, et al. (författare)
  • Minimizing the stop time of private vehicles at intersections with LRT signal priority systems
  • 2020
  • Ingår i: Transportation Research Procedia. - : Elsevier BV. - 2352-1465 .- 2352-1457. ; 48:2020, s. 939-945
  • Konferensbidrag (refereegranskat)abstract
    • There are some strategies suggested to improve the performance of intersections and increase the demand for public vehicles by prioritizing them. To this end, several methods have been used such as Transit Signal Priority (TSP) system for Light Rail transit (LRT). LRT signal priority is a timing strategy that gives priority to LRTs at signalized intersections through changing the sequence of phases, extending green time and reducing red time at LRT's phase. In this paper, we propose a model to improve LRT signal priority systems. The developed model minimizes the green extension and red reduction of LRT's phase by estimating an optimal speed for LRTs reaching the stop line. Consequently, the priority of LRTs would be maintained while the performance of private vehicles would be improved by decreasing their stop time.
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  • Resultat 1-3 av 3

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