| 1. |
- Uhlén, Mathias, et al.
(författare)
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A human protein atlas for normal and cancer tissues based on antibody proteomics
- 2005
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 4:12, s. 1920-1932
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Tidskriftsartikel (refereegranskat)abstract
- Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, similar to 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research.
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| 2. |
- Björklund, Anders, et al.
(författare)
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The Origins of Intergenerational Associations: Lessons from Swedish Adoption Data
- 2006
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Ingår i: Quarterly Journal of Economics. - : Oxford University Press (OUP). - 0033-5533 .- 1531-4650. ; 121:3, s. 999-1028
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Tidskriftsartikel (refereegranskat)abstract
- We use unique Swedish data with information on adopted children's biological and adoptive parents to estimate intergenerational mobility associations in earnings and education. We argue that the impact from biological parents captures broad prebirth factors, including genes and prenatal environment, and the impact from adoptive parents represents broad postbirth factors, such as childhood environment. We find that both pre- and postbirth factors contribute to intergenerational earnings and education transmissions, and that prebirth factors are more important for mother's education and less important for father's income. We also find some evidence for a positive interaction effect between postbirth environment and prebirth factors.
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| 3. |
- Björklund, Erik, et al.
(författare)
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Admission N-terminal pro-brain natriuretic peptide and its interaction with admission troponin T and ST segment resolution for early risk stratification in ST elevation myocardial infarction
- 2006
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Ingår i: Heart. - : BMJ Publishing Group. - 1468-201X .- 1355-6037. ; 92:6, s. 735-40
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVE: To assess the long term prognostic value of N-terminal pro-brain natriuretic peptide (NT-proBNP) on admission and its prognostic interaction with both admission troponin T (TnT) concentrations and resolution of ST segment elevation in fibrinolytic treated ST elevation myocardial infarction (STEMI). DESIGN AND SETTING: Substudy of the ASSENT (assessment of the safety and efficacy of a new thrombolytic) -2 and ASSENT-PLUS trials. PATIENTS: NT-proBNP and TnT concentrations were determined on admission in 782 patients. According to NT-proBNP concentrations, patients were divided into three groups: normal concentration (for patients < or = 65 years, < or = 184 ng/l and < or = 268 ng/l and for those > 65 years, < or = 269 ng/l and < or = 391 ng/l in men and women, respectively); higher than normal but less than the median concentration (742 ng/l); and above the median concentration. For TnT, a cut off of 0.1 microg/l was used. Of the 782 patients, 456 had ST segment resolution (< 50% or > or = 50%) at 60 minutes calculated from ST monitoring. MAIN OUTCOME MEASURES: All cause one year mortality. RESULTS: One year mortality increased stepwise according to increasing concentrations of NT-proBNP (3.4%, 6.5%, and 23.5%, respectively, p < 0.001). In receiver operating characteristic analysis, NT-proBNP strongly trended to be associated more with mortality than TnT and time to 50% ST resolution (area under the curve 0.81, 95% confidence interval (CI) 0.72 to 0.9, 0.67, 95% CI 0.56 to 0.79, and 0.66, 95% CI 0.56 to 0.77, respectively). In a multivariable analysis adjusted for baseline risk factors and TnT, both raised NT-proBNP and ST resolution < 50% were independently associated with higher one year mortality, whereas raised TnT contributed independently only before information on ST resolution was added to the model. CONCLUSION: Admission NT-proBNP is a strong independent predictor of mortality and gives, together with 50% ST resolution at 60 minutes, important prognostic information even after adjustment for TnT and baseline characteristics in STEMI.
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| 4. |
- Björklund, Erik, 1967-
(författare)
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Early Risk Stratification, Treatment and Outcome in ST-elevation Myocardial Infarction
- 2005
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- We evaluated, in patients with ST-elevation myocardial infarction (STEMI) treated with thrombolytics, admission Troponin T (tnT), ST-segment resolution and admission N-terminal pro-brain natriuretic peptide (NT-proBNP) for early risk stratification as well as time delays and outcome in real life patients according to prehospital or in-hospital thrombolytic treatment. Also, baseline characteristics, treatments and outcome in patients enrolled in the ASSENT-2 trial in Sweden and in patients not enrolled were evaluated.TnT (n=881) and NT-proBNP (n=782) on admission and ST-resolution at 60 minutes (n=516) in patients from the ASSENT-2 and ASSENT-PLUS trials were analysed. Elevated levels of NT-proBNP and tnT on admission were both independently related to one-year mortality. However, when adding information on ST-resolution (We investigated consecutive STEMI patients included in the RIKS-HIA registry between 2001 and 2004, if they were ambulance transported and had received prehospital (n=1690) or in-hospital (n=3685) thrombolytic treatment. Prehospital diagnosis and thrombolysis reduced the time to thrombolysis by almost one hour, were associated with better left ventricular function and fewer complications and reduced the adjusted one-year mortality by 30% compared with in-hospital thrombolysis.Prospective data from the RIKS-HIA registry on STEMI patients treated with thrombolytics were linked to data on trial participants in the ASSENT-2 trial of thrombolytic agents and used for direct comparisons. Patients treated with thrombolytics and not enrolled in a clinical trial at trial hospitals (n=2048) had higher risk characteristics, more early complications and twice as high adjusted one-year mortality compared to those enrolled (n=729). One major reason for the difference in outcome appeared to be the selection of less critically ill patients to the trial.
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| 5. |
- Björklund, Erik, et al.
(författare)
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Pre-hospital thrombolysis delivered by paramedics is associated with reduced time delay and mortality in ambulance-transported real-life patients with ST-elevation myocardial infarction
- 2006
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Ingår i: European Heart Journal. - : Oxford University Press (OUP). - 0195-668X .- 1522-9645. ; 27:10, s. 1146-1152
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Tidskriftsartikel (refereegranskat)abstract
- AIMS: There are sparse data on the impact of pre-hospital thrombolysis (PHT) in real-life patients. We therefore evaluated treatment delays and outcome in a large cohort of ambulance-transported real-life patients with ST-elevation myocardial infarction (STEMI) according to PHT delivered by paramedics or in-hospital thrombolysis. METHODS AND RESULTS: Prospective cohort study used data from the Swedish Register of Cardiac intensive care on patients admitted to the coronary care units of 75 Swedish hospitals in 2001-2004. Ambulance-transported thrombolytic-treated patients younger than age 80 with a diagnosis of acute myocardial infarction were included. Patients with PHT (n=1690) were younger, had a lower prevalence of co-morbid conditions, fewer complications, and a higher ejection fraction (EF) than in-hospital-treated patients (n=3685). Median time from symptom onset to treatment was 113 min for PHT and 165 min for in-hospital thrombolysis. One-year mortality was 7.2 vs. 11.8% for PHT and in-hospital thrombolysis, respectively. In a multivariable analysis, after adjusting for baseline characteristics and rescue angioplasty, PHT was associated with lower 1-year mortality (odds ratio 0.71, 0.55-0.92, P=0.008). CONCLUSION: When compared with regular in-hospital thrombolysis, pre-hospital diagnosis and thrombolysis with trained paramedics in the ambulances are associated with reduced time to thrombolysis by almost 1 h and reduced adjusted 1-year mortality by 30% in real-life STEMI patients.
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| 6. |
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| 7. |
- Björklund, Elisabet, et al.
(författare)
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Quality control of flow cytometry data analysis for evaluation of minimal residual disease in bone marrow from acute leukemia patients during treatment.
- 2009
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Ingår i: Journal of pediatric hematology/oncology : official journal of the American Society of Pediatric Hematology/Oncology. - : Lippincott Williams & Wilkins. - 1536-3678 .- 1077-4114. ; 31:6, s. 406-15
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Tidskriftsartikel (refereegranskat)abstract
- Low levels of leukemia cells in the bone marrow, minimal residual disease (MRD), are considered to be a powerful indicator of treatment response in acute lymphatic leukemia (ALL). A Nordic quality assurance program, aimed on standardization of the flow cytometry MRD analysis, has been established before implementation of MRD at cutoff level 10 as one of stratifying parameters in next Nordic Society of Pediatric Hematology and Oncology (NOPHO) treatment program for ALL. In 4 quality control (QC) rounds 15 laboratories determined the MRD levels in 48 follow-up samples from 12 ALL patients treated according to NOPHO 2000. Analysis procedures were standardized. For each QC round a compact disc containing data in list-mode files was sent out and results were submitted to a central laboratory. At cutoff level 10, which will be applied for clinical decisions, laboratories obtained a high concordance (91.6%). If cutoff level 10 was applied, the concordance would be lower (85.3%). The continuing standardization resulted in better concordance in QC3 and QC4 compared with QC1 and QC2. The concordance was higher in precursor B as compared with T-cell ALL. We conclude that after standardization, flow cytometry MRD detection can be reliably applied in international, multicenter treatment protocols.
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| 8. |
- Björklund, Henrik, 1973, et al.
(författare)
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Formal and informal communication in a project organisation: The case of a large construction project
- 2006
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Ingår i: Proceedings of IRNOP VII Project Research Conference, October 11-13, Xian, China. - 712103252X
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Konferensbidrag (refereegranskat)abstract
- In larger projects, where large numbers of project participants are involved, it is important to have a well functioning project organisation. The more complex a project is the more planning and control is needed. The study presented in this paper investigates an installation company’s formal and informal communication when constructing a new power plant for district heating, in Göteborg, Sweden. This is done in order to understand the informal communication and its effect on the project organisation. Literature reviews and interviews with managers involved in the project have been done. The results of the study indicate that the actual organisational structure differs from the planned structure in terms of communication paths. Actors involved, tend to contact each other beside the formal chain of order. A well functioning project team with established informal communication paths might be a possibility to achieve success in projects.
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| 9. |
- Björklund, Lars-Erik, 1953-
(författare)
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Från novis till expert : förtrogenhetskunskap i kognitiv och didaktisk belysning
- 2008
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Syftet med den här avhandlingen är att bidra till förståelsen av beprövad erfarenhetsbaserad kunskap, det vi förknippar med fårdigheter, förmågor och förtrogenhet. Avhandlingen är enintegrerande forskningsöversikt, fntegrative research review. Genom att sammanställa, jämföra och integrera resultat från psykologins, beteendevetenskapens, neuromedicinens och neurofysiologins forskningsfålt synliggör författaren en neurodidaktisk modell för hur experters egenskaper och förmågor byggs upp under praktisk erfarenhet i en specifik kontext.När en engagerad individ utsätts för en upprepad variation av upplevelser skapas kunskap som består av utfallsmärkta sensoriska mönster i framför allt de basala ganglierna men även i amygdala och cerebellum. Dessa sammantaget utgör ett implicit, omedvetet inlärningssystem parallellt med det medvetna explicita. Det implicita systemet jämför det vi betraktar med tidigare lagrade mönster vilket gör att vi snabbt kan upptäcka vad som är relevant i komplexa, stressade situationer. Med dess hjälp blir vi mer kompetenta i vår roll som lärare, hantverkare, läkare etc. Den här kunskapen blir omedveten, dold och tyst, det som Polanyi kallar Tacit knowledge och som bröderna Dreyfus refererar till som holistic pattern recognition. När vårt medvetande uppmärksammar ett igenkännande i det implicita systemet upplever vi en känsla, intuition eller gut feeling. Det implicita bedömningssystemet hämmas inte av arbetsminnets begränsningar i hastighet och antal detaljer och blir med tiden en resurs som visar sig som förtrogenhet inom en praktik. Mycket talar för att de mönster och erfarenheter som lagras i det implicita systemet används vid problemlösning. Detta leder till implikationer för hur undervisning och lärandemiljöer i exempelvis teknikämnet ska planeras för att befrämja en kreativ utveckling hos den lärande.Det implicita systemet ger oss en omedveten kapacitet att tolka andra människors kroppsspråk och aktiveras av kända kontexter och artefakter. Detta påverkar de svar vi som forskare får från våra informanter i en intervjusituation. För att nå expertens tysta kunskap om t.ex. bedömningskriterier kan en speciell intervjumetod; Repertory Grid Technique, användas.
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| 10. |
- Demandt, Marnie H
(författare)
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Biodiversity in Swedish Cyprinid Fish: Insights Into Processes of Divergence
- 2009
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Uncovering and understanding the processes that have led to the biological diversity we observe today are of fundamental interest in biology. Since direct observation of speciation is usually impossible, knowledge about the processes behind species formation can be gathered by studying mutations, natural/sexual selection, and genetic drift. In this thesis I aim to identify evolutionary processes that cause species divergence and, ultimately, speciation using Swedish cyprinid fish as a model system. Assuming that the demographic history of a population is mirrored in the genome, I studied the effects of a bottleneck on genetic variability in populations of roach. As expected, I found that a decrease in population size caused a decrease in genetic variability, a pattern that was obtained from both microsatellite and mitochondrial data. The importance of hybridization for speciation is debated, however, by analyzing morphology and microsatellites I could show that common bream and white bream and their interspecific hybrids are phenotypically and genetically differentiated and that ongoing geneflow is mainly unidirectional. Ongoing geneflow antagonizes the effect of genetic drift, but by studying isolated populations (= no gene flow) the impact of genetic drift can be assessed. Long-term isolated populations of roach and perch surprisingly showed stable levels of genetic diversity over time despite decreasing effective population size. However, each population genetically diverged during the period of investigation, a finding that is consistent with the effect of drift. An analysis of the systematic relationship of the 18 species of Swedish cyprinids revealed low congruence of phylogenies based on two different genetic markers. The position of the tench remains unresolved and the relationship of common bream and white bream as sister species cannot be confirmed. Within cyprinid fishes, diversification rates reveal a slowdown with time, a pattern that I found also in other fish clades and that is consistent with density-dependent cladogenesis. Overall, based on the findings presented in this thesis I emphasize that the maintenance of genetic variation in populations is essential since genetic variation is the key element for processes of divergence to act upon.
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