| 1. |
- Franzen, Johan, et al.
(författare)
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Regulation of apolipoprotein A1 synthesis in lymph drained rats
- 1987
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Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002. ; 918:1, s. 11-15
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Tidskriftsartikel (refereegranskat)abstract
- The effect of lymph diversion on plasma apolipoprotein A-I levels was studied. In lymph fistula rats apolipoprotein A-I levels in plasma stayed constant in spite of a loss of an equivalent of one-half plasma pool of apolipoprotein A-I per day through the lymph fistula. This indicates that synthesis of apolipoprotein A-I increases or that catabolism of apolipoprotein A-I decreases in a compensatory manner as intestinal apolipoprotein A-I is diverted. By using incorporation of [3H]leucine into newly synthesized apolipoprotein A-I it was shown that 2.6-times as much [3H]leucine was incorporated into apolipoprotein A-I in thoracic duct drained animals compared to controls. In experiments in which 125I-labeled HDL was injected intravenously into rats, it was shown that catabolism of HDL and apolipoprotein A-I was not decreased in lymph-drained rats. These data thus suggest that an increased synthesis of apolipoprotein A-I occurs when the intestinal contribution of apolipoprotein A-I diminishes. This is probably due to an increase in liver protein synthesis.
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| 2. |
- Jensen, Elmo, et al.
(författare)
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Cell density dependent uptake of chylomicron remnants in rat hepatocyte monolaayers. Effects of compactin and mevalonic acid.
- 1987
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Ingår i: Biochimica et Biophysica Acta. - : Elsevier BV. - 0006-3002. ; 917:1, s. 74-80
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Tidskriftsartikel (refereegranskat)abstract
- In rat hepatocytes cultured in lipoprotein-deficient serum, the uptake and degradation of chylomicron remnant cholesteryl ester per mg cell protein varies inversely with cell density. Compactin, a competitive inhibitor of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase, stimulates the uptake at all cell densities. Mevalonic acid, on the other hand, can suppress a significant part of the remnant uptake. Chylomicron remnant uptake in hepatocyte cultures can thus be influenced by factors known to regulate the apolipoprotein-BE receptor activity.
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| 3. |
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| 4. |
- Sjögren, Anders, et al.
(författare)
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Evaluation of zinc status in subjects with Crohn's disease
- 1988
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Ingår i: Journal of the American College of Nutrition. - 0731-5724. ; 7:1, s. 57-60
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Tidskriftsartikel (refereegranskat)abstract
- Zinc status was evaluated in 30 subjects with Crohn's disease. Intestinal resection had previously been performed in 23 of the subjects. The concentrations of zinc were determined in plasma, erythrocytes, percutaneous muscle biopsies, and in urine collected during 24 hours. The results were compared with those in 19 healthy controls. Most of the patients had a normal zinc status. The levels of zinc were, however, reduced (i.e., less than mean −2 SD for controls) in plasma for five, in erythrocytes for two, and in muscle biopsies for six subjects with Crohn's disease. The mean concentrations of zinc in plasma and erythrocytes were reduced (P less than 0.05), whereas the mean content of zinc in muscle biopsies and the mean urinary excretion of zinc were not significantly different, in subjects with Crohn's disease. The various zinc parameters did not correlate to each other. The results indicate that some subjects with Crohn's disease had an intracellular zinc depletion, which, however, was not reflected by a reduction in levels of zinc in plasma.
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| 5. |
- Sjögren, Anders, et al.
(författare)
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Magnesium and potassium status in healthy subjects as assessed by analysis of magnesium and potassium in skeletal muscle biopsies and of magnesium in mononuclear cells
- 1987
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Ingår i: Magnesium. ; 6:2, s. 91-99
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Tidskriftsartikel (refereegranskat)abstract
- Magnesium and potassium concentrations were determined in plasma, erythrocytes and urine collected during 24 h, and in percutaneous muscle biopsies obtained in 30 healthy subjects. Magnesium was also analyzed in mononuclear cells. Men, compared to women, showed a higher urinary excretion of magnesium (p less than 0.05). The magnesium contents in mononuclear cells correlated significantly with the concentrations of magnesium in muscle biopsies (r = 0.63; p less than 0.001) and with the urinary losses of magnesium (r = 0.40; p less than 0.05). A significant interrelationship between magnesium and potassium was found in striated muscle (r = 0.65; p less than 0.001), and the contents of potassium in muscle biopsies correlated significantly with the levels of potassium in plasma (r = 0.41; p less than 0.05). In a separate study the day-to-day variations for the magnesium and potassium parameters were investigated during 5 consecutive days in 7 healthy subjects. The coefficient of variation for magnesium in mononuclear cells was comparably low (8.0%), considering the wide range between the subjects. This indicates that magnesium in mononuclear cells might be a valuable parameter in longitudinal follow-up studies.
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| 6. |
- Sjögren, Anders, et al.
(författare)
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Magnesium, potassium and zinc deficiency in patients with type II diabetes mellitus
- 1988
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Ingår i: Acta Chemica Scandinavica. - 0904-213X. ; 224:5
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Tidskriftsartikel (refereegranskat)abstract
- The concentrations of magnesium, potassium and zinc were determined in plasma, erythrocytes, muscle biopsies, and in urine collected during 24 hours, in 18 subjects with type II diabetes mellitus (DM). Magnesium was also determined in mononuclear cells. The results were compared with those in 35 (magnesium and potassium analyses) or 26 (zinc analyses) healthy controls. Subjects with type II DM had lower concentrations of magnesium (3.79 +/- 0.32 vs. 4.29 +/- 0.22 mmol/100 g FFDS), potassium (40.5 +/- 5.17 vs. 46.1 +/- 3.81 mmol/100 g FFDS) and zinc (231 +/- 29 vs. 247 +/- 23 ng/mg FFDS) in skeletal muscle. Furthermore, the urinary excretions of magnesium and zinc were higher, as compared with those in healthy controls (5.00 +/- 2.68 vs. 3.62 +/- 1.47 mmol/24 hours, and 683 +/- 285 vs. 326 +/- 205 micrograms/24 hours, respectively). The contents of magnesium, potassium and zinc plasma did not correlate with the corresponding concentrations in skeletal muscle or circulating blood cells, as investigated in healthy controls, diabetics and in all subjects together, implying that the plasma concentrations are not useful in the assessment of electrolyte status. Hence, deficiency of electrolytes frequently occurs, and should be looked for, in subjects with type II DM.
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| 7. |
- Sjögren, Anders, et al.
(författare)
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Magnesium status in Crohns disease as evaluated by intracellular analysis and intravenous magnesium infusion
- 1988
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Ingår i: Scandinavian Journal of Gastroenterology. - : Informa UK Limited. - 1502-7708 .- 0036-5521. ; 23:5, s. 61-555
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Tidskriftsartikel (refereegranskat)abstract
- The magnesium status was evaluated in 30 subjects with Crohn's disease and in 30 controls. Subjects with Crohn's disease had lower concentrations of magnesium in muscle biopsy specimens (p less than 0.001), mononuclear cells (p less than 0.05), and in urine collected during 24 h (p less than 0.001) as compared with controls. After intravenous infusion of 60 mmol magnesium and 80 mmol potassium to 17 subjects with Crohn's disease a significant increase in concentrations of magnesium in muscle specimens (p less than 0.05), mononuclear cells (p less than 0.01), and plasma (p less than 0.01) was noted. The retention of intravenously infused magnesium was significantly higher in subjects with Crohn's disease than in 11 healthy controls (p less than 0.001) and was inversely correlated with the content of magnesium in muscle specimens (r = -0.52; p less than 0.05). The implication of these findings is that analysis of magnesium in muscle specimens and estimation of magnesium retention during an intravenous magnesium infusion are useful to confirm a suspected magnesium deficiency.
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