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| 2. |
- Hansson, Karin
(author)
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Characterization of a site-specific gene-transfer mechanism in bacteria
- 1998
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Doctoral thesis (other academic/artistic)abstract
- Integrons capture and rearrange single genes borne on mobile units called cassettes using site-specific recombination. The integrons increase the variation of antibiotic resistance among bacterial plasmids and may also reorganize clustered genes in bacterial genomes.The integrons consist of a conserved sequence coding for a recombinase of the lambda phage integrase family, and adjacent cassettes in tandem. Palindromic recombination sites, attC, occur 3' of all cassettes but the conserved sequence is joined to the cassettes by another type of site, attI. All sites contain a core with the reactive sequence GTT.Constructed deletions and point mutations were used to characterize attC and attI. Evidence was found that attC contains two symmetrical and co-operating subsites. The subsite containing the core was 1000-fold more reactive than that containing an inverse core. Mutations in the inverse core reduced recombination at the remote core strongly which suggests that the low-reactive end in attC has significance for the assembly of a synaptic nucleoprotein complex. The low-reactive end of attC could furthermore be used to fuse the site to sequences in the process of being recruited to new cassettes.Most integrations of cassettes occurred at attI. Recombination between two attI sites responded to upstream deletions progressing closer than 9-14 bp from the core. Recombination with an attC-site required another area of the conserved sequence 32 bp from the core of attI. The cross-over point was localized between G and TT in the core.Nucleotide sequencing of integrons documented new cassettes harboring ORFS, the dihydrofolate reductase gene dfr2a and a new spectinomycin resistance gene aadA4.The integron of class 2 in Tn7 was sequenced and functionality was demonstrated after mutagenesis of an internal stop codon in the integrase gene. Also the third class of integron was shown to be functional. Limited cross-specificity between the three integron classes was observed.The integrases of integron classes 1 and 3 were expressed as polyhistidine conjugates and purified by metal ion affinity chromatography. The purified recombinases catalyzed site-specific recombination in cell-free reactions stimulated by the architectural proteins HU and IHF.
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| 3. |
- Hansson, Karin
(author)
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Entering Heart of Darkness from a Postcolonial perspective. Teaching Notes
- 1998
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Reports (other academic/artistic)abstract
- The aim of this paper is twofold. First it contains a description how Heart of Darkness can be used in the definition and discussion of the terms imperialism and civilization. Second it describes a pattern of analysis based on the novella that is particularly relevant for postcolonial studies. Thus the novella, together with a selection of source material, serves both to give the historical and factual background necessary for the study of New Literatures in English and to provide the students with an analytical model that may be applied to other books in their reading list.
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| 4. |
- Hansson, Karin
(author)
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The Departure of the Author : A Post-Structuralist Reading of Gerald Murnane's Landscape with Landscape
- 1999
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Reports (other academic/artistic)abstract
- Gerald Murnane’s Landscape With Landscape is an intriguing book, full of contradictions and inconsistencies, teasing in the way it seems to be deliberately constructed to escape interpretation. Its anonymous, subversive and conceited first-person narrator cum protagonist, recognizable from other books by Murnane, is also the alleged writer of the six stories in the volume. In this capacity he gradually distances himself from his readers and finally, as will be further discussed, divests himself of all credibility. Thematically, truth and reality are key concepts but the narrative slides precariously between events that are ‘really’ taking place and a set of events that exist only in the deceptive narrator’s imagination. All the stories start with a definition of place, a vantage point or a scene of departure. Significantly, as the title indicates, place and spatial perspectives are far more important than time. Seemingly matter-of-fact introductory descriptions like “I am in the backyard of my three-bedroom house in the City of Heidelberg…” or “I stood on a hill northeast of Melbourne and looked across the folds of suburbs…” turn out to be just as unreliable as the person who defines them. What the narrator claims to be a Paraguayan setting turns out to be decidedly Australian, for example. Beneath and beyond what is usually defined as reality, depths and vistas of unreality are exposed. This indeterminacy related to landscapes and perspectives affects the reading experience making the interpretation of the text fraught with intriguing uncertainty.
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| 5. |
- Hansson, Karin
(author)
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The Unstable Manifold. Janet Frame´s Challenge to Determinism
- 1996
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Book (other academic/artistic)abstract
- The New Zealand writer Janet Frame has become known to the reading public mainly through her autobiographical trilogy comprising "To the Is-land", "An Angel at My Table", and "The Envoy from Mirror City". To a large extent critical attention has been autobiographically oriented, or otherwise focusing each of her eleven novels separately. This study concentrates on the theme of adaptation and evolution in her entire oeuvre. Applying a more interdisciplinary and scientific approach than is usually practised in leterary criticism, it considers Frame´s anti-Darwinian stance as "a Challenge to determinsim" calling material progress and adaptability into question. Novels like "Intensive Care", "Scented Gardens for the Blind", and "The Carpathians" are prophetic in picturing a cataclysmic end to human culture and civilization, it is true, but they also celebrate the power of imagination, memory and creativity. frame foresees not only horror and loneliness "at the edge of the alphabet" but also the possibility of a restoration of humanity and language after an era of unconditional adaptation.
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Journeys, Myths and the Age of Travel: Joseph Conrad’s Era
- 1998
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Book (other academic/artistic)abstract
- This critical collection, edited by Karin Hansson, contains papers given at an international conference organized by the Department of Humanities of the University of Karlskrona/Ronneby, Sweden in September 1997. To academic teachers and students with an interest in colonial of postcolonial studies, Joseph Conrad’s writing is central, historically, generically, and symbolically. The studies show how his oeuvre constitutes a common frame of reference internationally and how it relates in various ways to a number of writers, from his contemporaries to those of today. Illustrating the scope and variety of today’s criticism, the contributions deal with works from different phases of Conrad’s writing career, including his shorter fiction and novels as well as non-fiction.
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| 8. |
- Rengefors, Karin, et al.
(author)
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Algal cyst dormancy: a temporal escape from herbivory
- 1998
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In: PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES B-BIOLOGICAL SCIENCES. - 0962-8452. ; 265:1403, s. 1353-1358
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Journal article (peer-reviewed)abstract
- Many phytoplankton species form resting cysts and remain dormant for part of the year. The subsequent excystment is regulated by the external environment and internal maturation processes. Here we assessed the excystment of the dinoflagellates Ceratium hi
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| 9. |
- Swietlicki, Erik, et al.
(author)
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Source identification during the Great Dun Fell Cloud Experiment 1993
- 1997
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In: Atmospheric Environment. - 1352-2310. ; 31:16, s. 2441-2451
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Journal article (peer-reviewed)abstract
- A characterisation of the sources influencing the site for the final field campaign of the EUROTRAC subproject GCE (Ground-based Cloud Experiment) at Great Dun Fell, Cumbria, Great Britain in April-May 1993 is presented. The sources were characterised mainly by means of aerosol filter and cascade impactor data, single particle analysis, gas data, data on aromatic organic compounds, cloud water ionic composition, measurements of aerosol size distributions and hygroscopic properties and various meteorological information. Receptor models applied on the aerosol filter and impactor data sets separately revealed two major source types being a marine sea spray source and a long-range transported anthropogenic pollution source. The results of the receptor models were largely consistent with the other observations used in the source identification. Periods of considerable anthropogenic pollution as well as almost pure marine air masses were clearly identified during the course of the experiment.
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| 10. |
- Welander Hansson, U, et al.
(author)
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Nurses’ satisfaction with nursing care and work at three care units for severely demented people.
- 1995
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In: Journal of Psychiatric and Mental Health Nursing. - : Wiley. - 1351-0126 .- 1365-2850. ; 2:3, s. 151-158
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Journal article (peer-reviewed)abstract
- The aim of this study is to investigate the degree of job satisfaction, burnout and strain among nurses (n=134) working in three care units for demented people in southern Sweden. Three questionnaires were used: the Maslach burnout inventory (MBI), the work-related strain inventory (WRSI), and a recently developed measure for assessing job satisfaction. Based on principal component analysis, an eight-factor job satisfaction scale was chosen. Nurses in general were found to be satisfied, with registered nurses (RNs) being most satisfied with their work and the nursing care provided. The nurses in one of the care units were more satisfied than the nurses on the other two. The MBI and WRSI results showed a low degree of burnout and work-related strain and there was little difference between the care units or the categories of nurses. Job satisfaction scores were relatively independent from the MBI and WRSI scores. However, scores from these two inventories of burnout and strain at work were positively correlated. The results of the questionnaires seem to indicate that positive work experience and negative experience are not opposites. This particular method of evaluating job satisfaction needs further development. However, it seems important to include one specific measure for job satisfaction when action is taken in connection with the working situation of nurses.
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