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Seminal vesicles an...
Seminal vesicles and urinary bladder as sites of aromatization of androgens in men, evidenced by a CYP19A1-driven luciferase reporter mouse and human tissue specimens.
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Strauss, Leena (author)
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Rantakari, Pia (author)
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- Sjögren, Klara, 1970 (author)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research
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Salminen, Anu (author)
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Lauren, Eve (author)
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Kallio, Jenny (author)
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- Damdimopoulou, Pauliina (author)
- Karolinska Institutet
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Boström, Minna (author)
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Boström, Peter J (author)
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Pakarinen, Pirjo (author)
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Zhang, Fuping (author)
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Kujala, Paula (author)
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin och klinisk nutrition,Centre for Bone and Arthritis Research,Institute of Medicine, Department of Internal Medicine and Clinical Nutrition
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Mäkelä, Sari (author)
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- Poutanen, Matti (author)
- Gothenburg University,Göteborgs universitet,Centre for Bone and Arthritis Research
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(creator_code:org_t)
- 2012-12-13
- 2013
- English.
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In: The FASEB Journal. - : Wiley. - 0892-6638 .- 1530-6860. ; 27:4, s. 1342-50
- Related links:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
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- The human CYP19A1 gene is expressed in various tissues by the use of tissue-specific promoters, whereas the rodent cyp19a1 gene is expressed mainly in the gonads and brain. We generated a transgenic mouse model containing a >100-kb 5' region of human CYP19A1 gene connected to a luciferase reporter gene. The luciferase activity in mouse tissues mimicked the CYP19A1 gene expression pattern in humans. Interestingly, the reporter gene activity was 16 and 160 times higher in the urinary bladder and seminal vesicles, respectively, as compared with the activity in the testis. Accordingly, CYP19A1 gene and P450arom protein expression was detected in those human tissues. Moreover, the data revealed that the expression of CYP19A1 gene is driven by promoters PII, I.4, and I.3 in the seminal vesicles, and by promoters PII and I.4 in the urinary bladder. Furthermore, the reporter gene expression in the seminal vesicles was androgen dependent: Castration decreased the expression ∼20 times, and testosterone treatment restored it to the level of an intact mouse. This reporter mouse model facilitates studies of tissue-specific regulation of the human CYP19A1 gene, and our data provide evidence for seminal vesicles as important sites for estrogen production in males.-Strauss, L., Rantakari, P., Sjögren, K., Salminen, A., Lauren, E., Kallio, J., Damdimopoulou, P., Boström, M., Boström, P. J., Pakarinen, P., Zhang, F. P., Kujala, P., Ohlsson, C., Mäkelä, S., Poutanen, M. Seminal vesicles and urinary bladder as sites of aromatization of androgens in men, evidenced by a CYP19A1-driven luciferase reporter mouse and human tissue specimens.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Keyword
- aromatase
- male urogenital tract
- androgen regulation
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Strauss, Leena
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Rantakari, Pia
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Sjögren, Klara, ...
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Salminen, Anu
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Lauren, Eve
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Kallio, Jenny
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show more...
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Damdimopoulou, P ...
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Boström, Minna
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Boström, Peter J
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Pakarinen, Pirjo
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Zhang, Fuping
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Kujala, Paula
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Ohlsson, Claes, ...
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Mäkelä, Sari
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Poutanen, Matti
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and Endocrinology an ...
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MEDICAL AND HEAL ...
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The FASEB Journa ...
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University of Gothenburg
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Karolinska Institutet