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Träfflista för sökning "WFRF:(Lee Sang Sung) srt2:(2006-2009)"

Sökning: WFRF:(Lee Sang Sung) > (2006-2009)

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1.
  • Jo, Ji Song, et al. (författare)
  • Anti-wrinkle activity of beta-carotene extracted & purified from recombinant Escherichia coli
  • 2008
  • Ingår i: KSBB Journal. - 1225-7117 .- 2288-8268. ; 23:6, s. 513-518
  • Tidskriftsartikel (refereegranskat)abstract
    • This paper described the extraction/purification of β-carotene from recombinant E.coli and evaluation of anti-wrinkle activity of purified β-carotene. No significant differences in extraction yields were observed when hexane or isobutyl acetate was used. However, extraction from wet-cell cake resulted in 2-fold higher amount of β-carotene than that from dry cells. Disruption of 5 g-wet cells by ultrasonic homogenizer, acetone dehydration, extraction with isobutyl acetate resulted in 36 mg of β-carotene corresponding to 61.2% of recovery. The formation and separation of ββ-carotene crystal improved the purity. 633 mg of β-carotene crystal with 93% purity was obtained from 223 g/L of wet-cell cake harvested from 2.5-L fed-batch culture broth. The cultures of normal human primary fibroblast were performed to investigate the effect of β-carotene on cytotoxicity as MTT assay and anti-wrinkle activity as collagen synthesis assays. 1.7μM of β-carotene was found to be optimal concentration at which 1.4-fold higher amount of collagen was synthesized than that in absence of β-carotene. This indicates that highly purified β-carotene can be obtained from recombinant E.coli by applying simple method with less toxic solvent and can be used in functional cosmetics as anti-wrinkle agent.
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2.
  • Sung, Ki Won, et al. (författare)
  • Apparatus and Method for Reallocating Segments in Broadband Wireless Communication System
  • 2006
  • Patent (populärvet., debatt m.m.)abstract
    • An apparatus and a method for reallocating segments in a broadband wireless communication system are provided. An average density for all cells in system and an average minimal inter-cell distance R are computed. A circle is determined having a radius kR and a center at a corresponding cell by increasing a constant k until a density of the circle becomes less than or equal to the average density. A cluster is generated for each cell, which is composed of cells included in the determined circle. Accordingly, segment allocation capability can be improved while minimizing a width of segment reallocation.
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