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Sökning: WFRF:(Li Qian) > (2000-2004)

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1.
  • Cao, Jun, et al. (författare)
  • Helicobacter pylori-antigen-binding fragments expressed on the filamentous M13 phage prevent bacterial growth
  • 2000
  • Ingår i: Biochimica et Biophysica Acta - General Subjects. - 0304-4165 .- 1872-8006. ; 1474:1, s. 107-113
  • Tidskriftsartikel (refereegranskat)abstract
    • Colonization of the human stomach by Helicobacter pylori is associated with the development of gastritis, duodenal ulcer, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric cancer. H. pylori-antigen-binding single-chain variable fragments (ScFv) were derived from murine hybridomas producing monoclonal antibodies and expressed as a g3p-fusion protein on a filamentous M13 phage. The recombinant ScFv-phage reacted specifically with a 30-kDa monomeric protein of a H. pylori surface antigen preparation and by means of immunofluorescence microscopy the phage was shown to bind to both the spiral and coccoid forms of the bacterium. In vitro, the recombinant phage exhibited a bacteriocidal effect and inhibited specifically the growth of all the six strains of H. pylori tested. When H. pylori was pretreated with the phage 10 min before oral inoculation of mice, the colonization of the mouse stomachs by the bacterium was significantly reduced (P<0.01). The results suggest that genetic engineering may be used to generate therapy-effective phages.
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2.
  • Hu, Hanmei, et al. (författare)
  • Solvothermal synthesis of Sb2S3 nanowires on a large scale
  • 2003
  • Ingår i: Journal of Crystal Growth. - : Elsevier. - 0022-0248 .- 1873-5002. ; 258:1-2, s. 106-112
  • Tidskriftsartikel (refereegranskat)abstract
    • Sb2S3 nanowires with high aspect ratios have been successfully prepared on a large scale using SbCl3 and Na2S as starting materials in ethylene glycol at 200°C for 10 h. Field emission scanning electron microscopy images and transmission electron microscopy images show that the nanowires have diameters in the range of 20–100 nm and lengths up to 50 μm. Diffuse reflection spectrum indicates that the as-prepared Sb2S3 nanowires have obvious quantum size effects. The effects of reaction parameters on the growth of nanowires were discussed. A possible mechanism on the formation of the Sb2S3 nanowires was proposed.
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