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| 2. |
- Liu, Y X, et al.
(författare)
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Prolactin regulation of tissue type plasminogen activator and plasminogen activator inhibitor type-I gene expression in eCG-primed rat granulosa cells in culture.
- 1998
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Ingår i: Biology of Reproduction. - 0006-3363 .- 1529-7268. ; 59:2, s. 409-16
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Tidskriftsartikel (refereegranskat)abstract
- The present study was designed to investigate the effect of prolactin (PRL) on plasminogen activator inhibitor-I (PAI-I) and tissue type plasminogen activator (tPA) gene expression in eCG-primed granulosa cells in vitro. At 46 h after the hormone treatment, ovaries were removed, and granulosa cells were prepared for culture. Cells were incubated for various times in serum-free medium in the presence or absence of LH and PRL alone or in combination. tPA and PAI-I activities in the media were assayed by fibrin overlay and reverse fibrin autograph, respectively. Cytoplasmic RNA from granulosa cells was prepared using the NP-40 method and was assayed for PAI-I and tPA mRNA levels. We demonstrated the following. 1) PRL increased PAI-I mRNA production in cultured granulosa cells. Inclusion of LH with PRL had a synergistic effect on increasing PAI-I mRNA levels. After 48-h culture, 3-fold increases in PAI-I mRNA levels were seen with LH in combination with PRL as compared with PRL alone. The synergistic increase in PAI-I mRNA levels occurred in a dose- and time-dependent manner. 2) The increase in PAI-I mRNA synthesis by PRL alone, or by PRL in combination with LH, was well correlated with the changes in PAI-I activity and antigen levels in the conditioned media. 3) PRL in the culture also dramatically decreased LH-induced tPA mRNA and activity in a dose- and time-dependent fashion. The decrease in the tPA activity by PRL was also correlated with an increase in the amount of PA-PAI-I complexes in the cell-conditioned media. 4) In situ hybridization of tPA and PAI-I mRNAs in the cultured granulosa cells also showed that PRL was capable of enhancing PAI-I mRNA while diminishing tPA mRNA production induced by LH. This suggests that the dose- and time-dependent decrease in the gonadotropin-induced tPA activity in the culture by the presence of PRL may be due to decreasing tPA mRNA synthesis on one hand and to neutralization of the tPA activity by the increased PAI-I activity on the other.
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| 6. |
- Athas, W., et al.
(författare)
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AC-1: a clock-powered microprocessor
- 1997
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Ingår i: Proc of 1997 International Symposium on Low Power Electronics and Design. ; , s. 328-333
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Konferensbidrag (refereegranskat)
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| 7. |
- Berg, H, et al.
(författare)
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A neutron diffraction study of Ni substituted LiMn2O4
- 1998
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Ingår i: SOLID STATE IONICS. - : ELSEVIER SCIENCE BV. ; 112:1-2
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Annan publikation (övrigt vetenskapligt/konstnärligt)abstract
- X-ray acid neutron powder diffraction studies have been made on phase-pure LiNixMn2-xO4 (x = 0, 0.04, 0.18 and 0.33) substituted materials solely to establish how Ni substitutes into the spinel structure. Rietveld refinement showed all the materials to ha
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| 9. |
- Heyerdahl, H, et al.
(författare)
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Pharmacokinetic studies on 5-aminolevulinic acid-induced protoporphyrin IX accumulation in tumours and normal tissues
- 1997
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Ingår i: Cancer Letters. - 1872-7980. ; 112:2, s. 225-231
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Tidskriftsartikel (refereegranskat)abstract
- Laser-induced fluorescence (LIF) for in vivo point monitoring and fluorescence microscopy incorporating a CCD camera were used to study the fluorescence distribution of 5-aminolevulinic acid (ALA)-induced protoporphyrin IX (PpIX) in tumours. Fluorescence in a chemically induced adenocarcinoma in the liver of rats and in an aggressive basal cell carcinoma in a patient were studied after intravenous injection of ALA at a dose of 30 mg/kg body weight. The LIF technique demonstrated slightly more ALA-induced PpIX fluorescence in the tumour than in the surrounding normal liver and abdominal muscle of rats. The visible parts of the human basal cell carcinoma exhibited strong ALA-induced fluorescence, while this fluorescence was much weaker in the necrotic areas of the tumour and in the surrounding normal skin. (C) 1997 Elsevier Science ireland Ltd.
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