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Träfflista för sökning "WFRF:(Marie Rodolphe) srt2:(2005-2009);srt2:(2006)"

Search: WFRF:(Marie Rodolphe) > (2005-2009) > (2006)

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  • Marie, Rodolphe, et al. (author)
  • Use of PLL-g-PEG in micro-fluidic devices for localizing selective and specific protein binding
  • 2006
  • In: Langmuir. - : American Chemical Society (ACS). - 0743-7463 .- 1520-5827. ; 22:24, s. 10103-10108
  • Journal article (peer-reviewed)abstract
    • By utilizing flow-controlled PLL-g-PEG and PLL-g-PEGbiotin modification of predefined regions of a poly-(dimethylsiloxane) (PDMS) micro-fluidic device, with an intentionally chosen large (similar to 1 cm(2)) internal surface area, we report rapid (10 min), highly localized (6 x 10(-6) cm(2)), and specific surface-based protein capture from a sample volume (100 mu L) containing a low amount of protein (160 attomol in pure buffer and 400 attomol in serum). The design criteria for this surface modification were achieved using QCM-D (quartz crystal microbalance with energy dissipation monitoring) of serum protein adsorption onto PLL-g-PEG-modified oxidized PDMS. Equally good, or almost as good, results were obtained for oxidized SU-8, Topas, and poly(methyl metacrylate) (PMMA), demonstrating the generic potential of PLL-g-PEG for surface modification in various micro-fluidic applications.
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  • Result 1-3 of 3
Type of publication
conference paper (2)
journal article (1)
Type of content
peer-reviewed (3)
Author/Editor
Höök, Fredrik (3)
Marie, Rodolphe (3)
Tegenfeldt, Jonas (2)
Beech, Jason (1)
Sjövall, Peter (1)
Gunnarsson, Anders (1)
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Dahlin, Andreas (1)
Voeroes, Janos (1)
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University
Lund University (3)
Language
English (3)
Research subject (UKÄ/SCB)
Natural sciences (3)
Year

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