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- Abelson, Anna-Karin, et al.
(författare)
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STAT4 Associates with SLE through two independent effects that correlate with gene expression and act additively with IRF5 to increase risk
- 2009
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Ingår i: Annals of the Rheumatic Diseases. - : BMJ. - 0003-4967 .- 1468-2060. ; 68:11, s. 1746-1753
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Tidskriftsartikel (refereegranskat)abstract
- OBJECTIVES: To confirm and define the genetic association of STAT4 and systemic lupus erythematosus, investigate the possibility of correlations with differential splicing and/or expression levels, and genetic interaction with IRF5. METHODS: 30 tag SNPs were genotyped in an independent set of Spanish cases and controls. SNPs surviving correction for multiple tests were genotyped in 5 new sets of cases and controls for replication. STAT4 cDNA was analyzed by 5'-RACE PCR and sequencing. Expression levels were measured by quantitative PCR. RESULTS: In the fine-mapping, four SNPs were significant after correction for multiple testing, with rs3821236 and rs3024866 as the strongest signals, followed by the previously associated rs7574865, and by rs1467199. Association was replicated in all cohorts. After conditional regression analyses, two major independent signals represented by SNPs rs3821236 and rs7574865, remained significant across the sets. These SNPs belong to separate haplotype blocks. High levels of STAT4 expression correlated with SNPs rs3821236, rs3024866 (both in the same haplotype block) and rs7574865 but not with other SNPs. We also detected transcription of alternative tissue-specific exons 1, indicating presence of tissue-specific promoters of potential importance in the expression of STAT4. No interaction with associated SNPs of IRF5 was observed using regression analysis. CONCLUSIONS: These data confirm STAT4 as a susceptibility gene for SLE and suggest the presence of at least two functional variants affecting levels of STAT4. Our results also indicate that both genes STAT4 and IRF5 act additively to increase risk for SLE.
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| 2. |
- Gomez, Federico, et al.
(författare)
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Pulsed electric field reduces the permeability of potato cell wall
- 2008
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Ingår i: Bioelectromagnetics. - : Wiley. - 0197-8462 .- 1521-186X. ; 29:4, s. 296-301
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Tidskriftsartikel (refereegranskat)abstract
- The effect of the application of pulsed electric fields to potato tissue on the diffusion of the fluorescent dye FMI-43 through the cell wall was studied. Potato tissue was subjected to field strengths ranging from 30 to 500 V/cm, with one 1 ms rectangular pulse, before application of FM1-43 and microscopic examination. Our results show a slower diffusion of FM1-43 in the electropulsed tissue when compared with that in the non-pulsed tissue, suggesting that the electric field decreased the cell wall permeability. This is a fast response that is already detected within 30 s after the delivery of the electric field. This response was mimicked by exogenous H2O2 and blocked by sodium azide, an inhibitor of the production of H2O2 by peroxidases.
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| 4. |
- Vicente, Isabel Serrano, et al.
(författare)
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Action recognition and understanding through motor primitives
- 2007
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Ingår i: Advanced Robotics. - : Informa UK Limited. - 0169-1864 .- 1568-5535. ; 21:15, s. 1687-1707
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Tidskriftsartikel (refereegranskat)abstract
- In robotics, recognition of human activity has been used extensively for robot task learning through imitation and demonstration. However, there has not been much work performed on modeling and recognition of activities that involve object manipulation and grasping. In this work, we deal with single arm/hand actions which are very similar to each other in terms of arm/hand motions. The approach is based on the hypothesis that actions can be represented as sequences of motion primitives. Given this, a set of five different manipulation actions of different levels of complexity are investigated. To model the process, we use a combination of discriminative support vector machines and generative hidden Markov models. The experimental evaluation, performed with 10 people, investigates both the definition and structure of primitive motions, as well as the validity of the modeling approach taken.
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