| 1. |
- Andersch-Björkman, Ylva, et al.
(författare)
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Air-oxidized linalool elicits eczema in allergic patients - a repeated open application test study.
- 2014
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Ingår i: Contact dermatitis. - : Wiley. - 1600-0536 .- 0105-1873. ; 70:3, s. 129-38
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Tidskriftsartikel (refereegranskat)abstract
- Linalool is a commonly used fragrance terpene that forms potent sensitizers upon oxidation. In a recent multicentre study, we found that 7% of 2900 patients showed positive patch test reactions to oxidized linalool at 6.0%. No elicitation studies have been performed.
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| 2. |
- Blixt, Martin, et al.
(författare)
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Pancreatic islets of bank vole show signs of dysfunction after prolonged exposure to high glucose concentrations in vitro
- 2010
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Ingår i: Journal of Endocrinology. - 0022-0795 .- 1479-6805. ; 206:1, s. 47-54
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Tidskriftsartikel (refereegranskat)abstract
- Bank voles develop glucose intolerance/diabetes mellitus when kept in captivity. We have characterized beta-cell function of glucose intolerant/diabetic animals, and found that this animal model has features of both human type 1 and type 2 diabetes. The aim of this study was to study the functional alterations of islets isolated from glucose tolerant bank voles after a prolonged exposure to various glucose concentrations in vitro. For this purpose, pancreatic islets from normal (glucose tolerant) male and female bank voles were cultured at different glucose concentrations (5.6, 11.1 (control), or 28 mM) whereupon islet functions were examined. Overall, islet insulin output was lowered at 5.6 mM glucose, and similar to control, or enhanced after culture in 28 mM glucose. High glucose culture led to decreased insulin contents, but there was no change in islet DNA content and in morphological assessments of cell death, with the latter findings suggesting that the so-called glucotoxicity had not evolved. A slight gender difference was observed in that islets isolated from females exhibited a glucose-regulated (pro) insulin biosynthesis rate and insulin gene expression. In conclusion, we have found that islets isolated from female and male bank voles are affected by glucose concentrations in vitro in that some signs of dysfunction were observed upon high glucose exposure. A minor gender difference was observed suggesting that the islets of the females may more readily adapt to the elevated glucose concentration than islets of the male bank voles. It could be that these in vitro gender differences observed may represent a mechanism underlying the gender difference in diabetes development observed among bank voles. Journal of Endocrinology (2010) 206, 47-54
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| 3. |
- Escadafal, Camille, et al.
(författare)
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Second External Quality Assurance Study for the Serological Diagnosis of Hantaviruses in Europe
- 2012
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Ingår i: PLoS Neglected Tropical Diseases. - : Public Library of Science (PLoS). - 1935-2735. ; 6:4, s. e1607-
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Tidskriftsartikel (refereegranskat)abstract
- Hantaviruses are endemic throughout the world and hosted by rodents and insectivores. Two human zoonoses, hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS), are caused by hantaviruses and case fatality rates have reached 12% for HFRS and 50% for HPS in some outbreaks. Symptomatic hantavirus infections in Europe are summarised as HFRS mainly due to Puumala, Dobrava-Belgrade and Saaremaa virus. While HFRS has an overall low incidence in Europe, the number of cases varies from 100 per year in all Eastern and Southern Europe up to 1,000 per year only in Finland. To assess the quality of hantavirus diagnostics, the European Network for the Diagnostics of 'Imported'' Viral Diseases (ENIVD) organised a first external quality assurance (EQA) in 2002. The purpose of this second EQA study is to collect updated information on the efficiency and accurateness of hantavirus serological methods applied by expert laboratories. A serum panel of 14 samples was sent to 28 participants in Europe of which 27 sent results. Performance in hantavirus diagnosis varied not only on the method used but also on the laboratories and the subclass of antibodies tested. Commercial and in-house assays performed almost equally. Enzyme immunoassays were mainly used but did not show the best performances while immunoblot assays were the less employed and showed overall better performances. IgM antibodies were not detected in 61% of the positive IgM samples and IgM detection was not performed by 7% of the laboratories indicating a risk of overlooking acute infections in patients. Uneven performances using the same method is indicating that there is still a need for improving testing conditions and standardizing protocols.
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| 4. |
- Hauffe, Heidi C, et al.
(författare)
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Ljungan Virus Detected in Bank Voles (Myodes glareolus) and Yellow-Necked Mice (Apodemus flavicollis) from Northern Italy
- 2010
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Ingår i: Journal of Wildlife Diseases. - 0090-3558 .- 1943-3700. ; 46:1, s. 262-266
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Tidskriftsartikel (refereegranskat)abstract
- Identified in 1998, Ljungan virus (LV; Picornaviridae) causes type 1 diabetes-like symptoms and myocarditis in bank voles (Myodes glareolus) from Sweden and Denmark, and may be a zoonotic agent of several important diseases (e.g., intrauterine fetal death, I diabetes, Guillain-Barre syndrome, and myocarditis). Using a real-time reverse transcriptase-polymerase chain reaction assay and sequence anlalysis, we detected LV in bank voles, and for the first time, in yellow-necked mice collected during 2006 from a site in northern Italy. The global distribution of LV and its role as a mammalian pathogen deserve further attention.
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| 5. |
- Johansson, Jörgen, et al.
(författare)
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"Kommunernas region" : Kommunernas inflytande i en sydsvensk storregion
- 2014
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Ingår i: Behöver vi en sydsvensk region?. - Kristianstad : Region Skåne. - 9789163764875 ; , s. 91-112
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Vilka möjligheter till verksamhetsutveckling ser kommunerna i de fyra berörda länen (Blekinge, Kalmar, Kronoberg och Skåne)? Vilka vinster kan kommunerna göra om länen slås samman och kommunerna får ett inflytande över den nya regionorganisationen? Vilka samverkansmöjlig-heter finns mellan kommunerna och mellan kommun och region? Vilken organisationsform krävs för att samverkan ska utvecklas på ett positivt sätt?Vi vill med andra ord analysera hur kommunerna förhåller sig till en uppväxlad regional nivå, i synnerhet inom tillväxtpolitikens område. Vi är intresserade både av frågor som rör organiseringen av regionnivån, och kommunernas roll i denna, och av hur kommunerna tror att en storregionreform kommer att påverka kommunernas handlingsutrymme och samverkan med varandra.När Region Skåne bildades var det en viktig utgångspunkt att kommunerna fick ett inflytande i den nya organisation (landstinget) som övertog det regionala utvecklingsansvaret. I många län sker nu ett samgående mellan landsting och regionförbund med utvecklingsansvar, vilket väcker frågan om vilken roll kommunerna bör få i förhållande till den nya regionorganisationen, ibland kallad regionkommun. Frågan gäller den nya Region Kronoberg men kan ställas om alla fyra berörda län vid en eventuell sammanslagning.
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| 6. |
- Johansson, Jörgen, 1958-, et al.
(författare)
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"Kommunernas region" – kommunernas inflytande i en sydsvensk storregion
- 2014
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Ingår i: Behöver vi en sydsvensk region?. - : Sydsvensk regionbildning. - 9789163764875 ; , s. 91-112
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Vilka möjligheter till verksamhetsutveckling ser kommunerna i de fyra berörda länen (Blekinge, Kalmar, Kronoberg och Skåne)? Vilka vinster kan kommunerna göra om länen slås samman och kommunerna får ett inflytande över den nya regionorganisationen? Vilka samverkansmöjligheter finns mellan kommunerna och mellan kommun och region? Vilken organisationsform krävs för att samverkan ska utvecklas på ett positivt sätt?
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| 7. |
- Kallies, Rene, et al.
(författare)
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Development and characterization of murine monoclonal antibodies to first and second Ljungan virus genotypes
- 2012
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Ingår i: Journal of Virological Methods. - : Elsevier BV. - 0166-0934 .- 1879-0984. ; 184:1-2, s. 27-33
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Tidskriftsartikel (refereegranskat)abstract
- Ljungan virus (LV) is a rodent pathogen that causes diabetes and myocarditis in its natural host. In addition, LV has been associated with human disease during pregnancy and of neonates, respectively. A panel of 22 monoclonal antibodies (mAbs) against first and second LV genotypes were produced by immunization of BALB/c mice with whole virus. Thirteen mAbs were class IgG antibodies and nine were class IgM antibodies; all of them contained kappa light chains. All mAbs were reactive with LV by capture enzyme-linked immunosorbent assay and indirect immunofluorescence assay. In addition, five mAbs showed a positive staining in immunohistochemistry. No mAb bound to denatured capsid proteins detected by western immunoblotting. In contrast, the target capsid protein(s) of 20 mAbs were identified by immune precipitation, revealing the conformational nature of epitopes required for mAb binding. None of the mAbs reacted with third and fourth LV genotypes. mAbs characterized should provide useful tools for the development of diagnostic assays and the investigation of LV first and second genotype properties and its pathogenesis.
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| 8. |
- Litzba, Nadine, et al.
(författare)
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Evaluation of Different Serological Diagnostic Methods for Tick-Borne Encephalitis Virus : Enzyme-Linked Immunosorbent, Immunofluorescence, and Neutralization Assay
- 2014
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Ingår i: Vector Borne and Zoonotic Diseases. - : Mary Ann Liebert Inc. - 1530-3667 .- 1557-7759. ; 14:2, s. 149-159
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Tidskriftsartikel (refereegranskat)abstract
- Tick-borne encephalitis (TBE) is a zoonotic disease, transmitted mainly by the bite of ticks. The TBE virus (TBEV) belongs to the family Flaviviridae, genus Flavivirus and is able to cause meningoencephalitis. For serological TBEV detection, the neutralization test (NT) is the most specific assay available. Different NT protocols are used in the laboratories, and until now the performance of these NTs has never been tested in an external quality assessment (EQA). In this EQA, we compared the results of eight European laboratories in detecting 17 samples (11 TBEV positive, five flavivirus cross reactive, and one negative sample) by NT. Furthermore, 14 of these EQA samples and 15 additional samples were tested in different commercial assays: 15 immunoglobulin G (IgG) enzyme-linked immunosorbent assays (ELISAs) and an immunofluorescence assay (IFA). Four laboratories showed a good NT EQA performance, whereas four laboratories had some sensitivity problems. Additionally, two of these laboratories showed a lack in specificity, misidentifying a dengue-positive sample as TBEV positive. The comparison of the commercial ELISAs revealed a high sensitivity in all assays, but as expected for IgG, the ELISAs showed a high degree of flavivirus cross reactivity. The assessment of Vienna Units in some of the ELISAs revealed deviations in the standards used by the different companies. Therefore, these standards should be revised. Generally, in this EQA, we found that reliable NT protocols are used in most of the laboratories, and the evaluation of the IgG ELISAs and the IFA showed a good agreement.
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| 9. |
- Niklasson, Gunnar A., 1953-, et al.
(författare)
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Dielectric Properties of Water-Containing Porous Materials : Low Frequency Response of Sandstones
- 2013
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Ingår i: ISEMA 2013. - Weimar, Germany : MFPA Weimar. ; , s. 195-202
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Konferensbidrag (refereegranskat)abstract
- We present results of dielectric spectroscopy measurements on natural sandstones, where the pores were impregnated with water solutions of different conductivity. The measurements were fitted to an equivalent circuit containing a high-frequency Maxwell-Wagner relaxation and a low frequency response due to ion conduction and relaxation in the electrochemical double layer at the interfaces between the solid grains and the water. We propose a novel expression for the low frequency response based on a transmission line model. We also study in detail the shape of the Maxwell-Wagner relaxation in samples of porous polypropylene impregnated with transformer oil.
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