| 1. |
- Nilsson, J, et al.
(författare)
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Competitive elution of protein A fusion proteins allows specific recovery under mild conditions.
- 1994
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Ingår i: European Journal of Biochemistry. - : Wiley. - 0014-2956 .- 1432-1033. ; 224:1, s. 103-8
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Tidskriftsartikel (refereegranskat)abstract
- A novel system is described for mild elution of fusion proteins by competitive elution. The approach is based on displacement of immobilized fusions containing a monovalent IgG-binding staphylococcal protein A fragment (Z) from an IgG-affinity matrix by a divalent fragment fused to a serum-albumin-binding region derived from streptococcal protein G. Using real-time interaction analysis, the binding (K(aff)) to polyclonal human IgG was found to be 3.3 (+/- 0.4) x 10(8) M-1 for divalent ZZ and 2.0 (+/- 0.1) x 10(7) M-1 for monovalent Z. This more than tenfold difference in binding strength ensures a high efficiency in the elution step. The competitor protein can specifically be removed and recovered from the elution mixture by subsequent passage through a human serum albumin(HSA)-affinity column, leaving only the target fusion protein in the flow-through fraction. Here, we show that a recombinant Klenow fragment of DNA polymerase I expressed in Escherichia coli can be recovered with high yield, and retained activity, from a crude bacterial lysate by IgG-affinity chromatography using mild conditions during both binding and elution.
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| 2. |
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| 3. |
- Nygren, Else, et al.
(författare)
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Reading the medical record.I. Analysis of physicians ways of reading the medical record
- 1992
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Ingår i: Computer Methods and Programs in Biomedicin. ; 39, s. 1992-
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Tidskriftsartikel (refereegranskat)abstract
- Physicians were interviewed about their routines in everyday use of the medical record. From the interviews, we conclude that the medical record is a well functioning working instrument for the experienced physician. Using the medical record as a basis for decision making involves interpretation of format, layout and other textual features of the type-written data. Interpretation of these features provides effective guidance in the process of searching, reading and assessing the relevance of different items of information in the record. It seems that this is a skill which is an integrated part of diagnostic expertise. This skill plays an important role in decision making based on the large amount of information about a patient that can be found in the medical record. This finding has implications for the design of user interfaces for reading computerized medical records.
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| 4. |
- Nygren, Else, et al.
(författare)
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Reading the medical record II. Design of a human-computer interface for basic reading of computerized medical records.
- 1992
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Ingår i: Computer Methods and Programs in Biomedicine. ; 39, s. 13-25
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Tidskriftsartikel (refereegranskat)abstract
- A user interface for reading the medical record was designed and implemented on a work-station with a 19 inch colour screen. The text is presented on imitations of paper-pages. The pages are organized in bundles which are dynamically connected to scrollable index-lists. The turning of pages on the screen is the fundamental concept of the interface. A page can be turned by a mouse-click or by a circular mouse-movement. Elaborated feedback is given to the user in order to provide effortless orientation and navigation. The interface supports the basic ways of use identified in our analyses of reading habits. It also enables human perceptual and cognitive skills to be used. It seems very easy to learn and efficient in use.
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