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| 2. |
- Dimberg, Anna, et al.
(författare)
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alphaB-crystallin promotes tumor angiogenesis by increasing vascular survival during tube morphogenesis
- 2008
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Ingår i: Blood. - : American Society of Hematology. - 0006-4971 .- 1528-0020. ; 111:4, s. 2015-2023
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Tidskriftsartikel (refereegranskat)abstract
- Selective targeting of endothelial cells in tumor vessels requires delineation of key molecular events in formation and survival of blood vessels within the tumor microenvironment. To this end, proteins transiently up-regulated during vessel morphogenesis were screened for their potential as targets in antiangiogenic tumor therapy. The molecular chaperone alpha B-crystallin was identified as specifically induced with regard to expression level, modification by serine phosphorylation, and subcellular localization during tubular morphogenesis of endothelial cells. Small interfering RNA-mediated knockdown of alpha B-crystallin expression did not affect endothelial proliferation but led to attenuated tubular morphogenesis, early activation of proapoptotic caspase-3, and increased apoptosis. alpha B-crystallin was expressed in a subset of human tumor vessels but not in normal capillaries. Tumors grown in alpha B-crystallin(-/-) mice were significantly less vascularized than wild-type tumors and displayed increased areas of apoptosis/necrosis. Importantly, tumor vessels in alpha B-crystallin(-/-) mice were leaky and showed signs of caspase-3 activation and extensive apoptosis. Ultrastructural analyses showed defective vessels partially devoid of endothelial lining. These data strongly implicate alpha B-crystallin as an important regulator of tubular morphogenesis and survival of endothelial cell during tumor angiogenesis. Hereby we identify the small heat shock protein family as a novel class of anglogenic modulators.
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| 3. |
- Fornara, Andrea, et al.
(författare)
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Tailored Magnetic Nanoparticles for Direct and Sensitive Detection of Biomolecules in Biological Samples
- 2008
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Ingår i: Nano letters (Print). - : American Chemical Society (ACS). - 1530-6984 .- 1530-6992. ; 8:10, s. 3423-3428
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Tidskriftsartikel (refereegranskat)abstract
- We developed nanoparticles with tailored magnetic properties for sensitive detection of biomolecules directly in biological samples in a single step. Thermally blocked nanoparticles obtained by thermal hydrolysis are mixed with sample solutions and the variation of the magnetic relaxation due to surface binding is used to detect the presence of biomolecules. The binding events significantly increase the hydrodynamic volume of nanoparticles, thus changing their Brownian relaxation frequency which is measured by a specifically developed AC-susceptometer.The system was tested for the presence of Brucella antibodies in serum samples from infected cows and the surface of the nanoparticles was functionalized with lipopolysaccarides (LPS) from Brucella abortus. The hydrodynamic volume of functionalized particles increased by 25-35% as a result of the binding of the antibodies, as measured by changes in the susceptibility in an alternating magnetic field. The method has shown high sensitivity, with detection limit of 7 nmol·L-1 in serum without any pre-treatment of the biological samples.The detection method is very sensitive, cost-efficient and versatile, giving a direct indication if the animal is infected or not, making it suitable for point-of care applications. The functionalization of tailored magnetic nanoparticles can be modified to suit numerous homogenous assays for a wide range of applications.
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| 4. |
- Jansson, Agneta, 1973-, et al.
(författare)
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A new polymorphism in the coding region of exon four in HSD17B2 in relation to risk of sporadic and hereditary breast cancer
- 2007
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Ingår i: Breast Cancer Research and Treatment. - : Springer Science and Business Media LLC. - 0167-6806 .- 1573-7217. ; 106:1, s. 57-64
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Tidskriftsartikel (refereegranskat)abstract
- In situ synthesis of oestrogens is of great importance in the development and progression of breast cancer. 17β-hydroxysteroid dehydrogenase (17HSD) type 2 catalyses oxidation from oestradiol to oestrone, and thereby protects the breast epithelial cells from oestradiol. Low expression of 17HSD type 2 has been associated with decreased survival in breast cancer, but no studies have investigated the mechanism behind the low expression. The 17HSD type 2 gene (HSD17B2) was screened for mutations with Single Stranded Conformation Polymorphism (SSCP)-DNA sequencing in 59 sporadic breast cancer cases, 19 hereditary breast cancer cases and seven breast cancer cell lines. DNA samples from 226 healthy individuals were used to identify if changes were previously unknown polymorphisms. No mutation was detected and therefore mutations in HSD17B2 do not explain why some breast tumours exhibit low 17HSD type 2 expression. However, a previously unknown polymorphism was found in exon four (Met226Val). Using molecular modelling, we found that the substituted residue is located at the outer part of the steroid binding site, probably causing minor alterations in the substrate binding. We further studied if the polymorphism contributes to breast cancer susceptibility in a larger material, but did not find an increased risk in the group of 317 sporadic breast cancer patients, 188 breast cancer patients with two close relatives with breast cancer or 122 hereditary breast cancer patients, compared to the healthy control group. We suggest that the detected polymorphism does not contribute to a higher risk of developing breast cancer.
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| 5. |
- Johansson, Lars-Olof, 1957, et al.
(författare)
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Weighing third-party fairness, efficiency, and self-interest in resource allocation decisions
- 2007
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Ingår i: Journal of Economic Psychology. ; , s. 53-68
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Tidskriftsartikel (refereegranskat)abstract
- In two experiments participants allocated a resource between themselves, one privileged, and one unprivileged group. Conflicts were induced between “third party fairness” (difference in payoff to the two groups) and efficient resource use. Experiment 1 employed 42 undergraduates making multi-stage allocation decisions. Fairness was varied at three levels and self-interest, operationalized as the political motive of win votes, at two levels. Adverse effects of fairness on preserving the resource were found. In Experiment 2 another 48 undergraduates performed a similar task. Self-interest was now operationalized as a financial motive. The results showed that participants reduced their resource use when neither fairness nor self-interest was salient. A regression analysis demonstrated consistent adverse effects of fairness on resource use controlling for efficient resource use and total group payoff. It was concluded that fairness causes decision makers to overuse resources, although they are unwilling to totally deplete a resource and will make compromises.
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| 6. |
- Johansson, Petter, et al.
(författare)
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Failure to detect mismatches between intention and outcome in a simple decision task
- 2005
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Ingår i: Science. - : American Association for the Advancement of Science (AAAS). - 1095-9203 .- 0036-8075. ; 310:5745, s. 116-119
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Tidskriftsartikel (refereegranskat)abstract
- A fundamental assumption of theories of decision-making is that we detect mismatches between intention and outcome, adjust our behavior in the face of error, and adapt to changing circumstances. Is this always the case? We investigated the relation between intention, choice, and introspection. Participants made choices between presented face pairs on the basis of attractiveness, while we covertly manipulated the relationship between choice and outcome that they experienced. Participants failed to notice conspicuous mismatches between their intended choice and the outcome they were presented with, while nevertheless offering introspectively derived reasons for why they chose the way they did. We call this effect choice blindness.
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| 7. |
- Olsson, Lars E., 1971, et al.
(författare)
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Examining the Use of Subsidies for the Abatement of Greenhouse Gas Emissions through Experimental Simulations
- 2006
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Ingår i: European Environment. ; , s. 184-197
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Tidskriftsartikel (refereegranskat)abstract
- A market experiment was designed to empirically investigate the potential effectiveness of a governmental subsidy system to reduce sales and therefore production of environmentally harmful products. The important issue of whether the subsidy system preserves competitiveness was also examined. In the experiment two levels of a subsidy for unsold units were compared with no subsidy. To simulate the way in which subsidy levels may vary across time in real markets, the effects of high and low uncertainty regarding the subsidy level were also investigated. The results showed that subsidies, whether known and fixed or uncertain and varying, did not erode competition but nevertheless led to higher prices, which resulted in fewer sales. In the control condition a price war resulting in decreasing prices and increasing sales were observed. Several ways in which the proposed subsidy system may be implemented in the transport sector and other sectors are discussed. It is suggested that subsidies may make the adjustment process toward sustainable production less costly for the regulated parties.
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| 8. |
- Olsson, Lars E., 1971, et al.
(författare)
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Experimental simulations of a subsidy system for sustainable production
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- A multi-trial duopoly price-setting experiment was conducted to investigate the potential effectiveness of a governmental subsidy system aimed to reduce sales and therefore production of environmentally harmful products. Two levels of a subsidy for unsold units were compared with a control condition without a sub-sidy. The results showed that the subsidies did not erode competition but never-theless led to the setting of higher prices that resulted in fewer sales. In the con-trol condition a price-war led to decreasing prices and increasing sales. The ways in which the proposed subsidy system may be implemented in the transport sec-tor and other sectors are discussed. Specifically, it is proposed that the subsidy system may complement the newly introduced European Emission Trading Scheme.
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| 9. |
- Olsson, Lars E., 1971, et al.
(författare)
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Experimental tests of system of governmental subsidies for sustainable production.
- 2005
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Ingår i: Paper presented at the 7th Nordic Environmental Social Science (NESS) research conference, Göteborg University, Sweden.
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Konferensbidrag (refereegranskat)abstract
- A multi-trial duopoly price-setting game was used to investigate the potential ef-fectiveness of a governmental subsidy system to reduce sales and therefore pro-duction of environmentally harmful products. Two levels of a subsidy for unsold units were compared with a control condition without a subsidy. The results showed that the subsidies did not prevent competition but nevertheless led to the setting of higher prices that resulted in fewer sales. In the control condition a price war led to decreasing prices and increasing sales. The ways in which the proposed subsidy system may be implemented in the transport sector and other sectors are discussed. Specifically, it is proposed that the subsidy system may complement the newly introduced European Emission Trading Scheme
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| 10. |
- Olsson, Lars E., 1971, et al.
(författare)
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Subsidies for sustainable production: Effects of changing subsidy level
- 2005
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- A multi-trial duopoly price-setting game was used to investigate the potential ef-fectiveness of a subsidy system aimed at reducing sales and thereby production of environmentally harmful products. In real life it is however likely that a subsidy level will never remain fixed, either nominally or in real value. Producers may also have unreliable perceptions of the subsidy level even if it does not change. Therefore, a subsidy, either fixed and known, varying randomly, or varying sys-tematically across trials, was investigated. The results showed that both fixed and known, as well as varying subsidy levels led to higher prices and thus re-duced sales compared to without a subsidy. Knowledge of a fixed subsidy level or expectations about a subsidy level based on previous trials, appeared to make participants refrain from setting lower prices than the subsidy level. Further-more, the results indicated that participants attempted to maximize their profit by selling to the lowest price, thus they stayed competitive and did not exploit the subsidy.
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