SwePub
Sök i SwePub databas

  Utökad sökning

Träfflista för sökning "WFRF:(Persson Carl) srt2:(2005-2009)"

Sökning: WFRF:(Persson Carl) > (2005-2009)

  • Resultat 1-10 av 56
Sortera/gruppera träfflistan
   
NumreringReferensOmslagsbildHitta
1.
  • Ahlström-Emanuelsson, Cecilia, et al. (författare)
  • Effects of topical formoterol alone and in combination with budesonide in a pollen season model of allergic rhinitis.
  • 2007
  • Ingår i: Respiratory Medicine. - : Elsevier BV. - 1532-3064 .- 0954-6111. ; 101:6, s. 1106-1112
  • Tidskriftsartikel (refereegranskat)abstract
    • Background: beta(2)-Agonists may exert mast cell stabilizing and anti-plasma exudation effects. White available data suggest no or only marginal effects of beta(2)-agonists on symptoms of allergic rhinitis, little is known about whether these drugs may add to the efficacy of anti-rhinitis drugs. Objective: To examine effects of a beta(2)-agonist, alone and in combination with an intranasal glucocorticosteroid, on symptoms and signs of allergic rhinitis. Methods: Patients were examined in a pollen season model. Budesonide 64 mu g, alone and in combination with formoterot 9 mu g, as well as formoterot 9 mu g alone was given in a placebo-controlled and crossover design. After 7 days of treatment, the patients received allergen challenges for 7 days. Symptoms and nasal peak inspiratory flow (PIF) were recorded. Nasal lavages with and without histamine were carried out at the end of each challenge series. These lavages were analysed for tryptase, eosinophil cationic protein (ECP), and alpha(2)-macroglobutin as indices of mast cell activity, eosinophil activity, and plasma exudation, respectively. Results: Budesonide reduced symptoms of allergic rhinitis and improved nasal PIF in the morning, in the evening as well as post allergen challenge. Formoterol alone did not affect symptoms or nasal PIF and did not affect the efficacy of budesonide. Tryptase, ECP, and alpha(2)-macroglobutin were significantly reduced by budesonide. Formoterol alone did not affect these indices and did not affect the anti-inflammatory effect of budesonide. Conclusion: The present dose of formoterot does not affect symptoms and inflammatory signs of allergic rhinitis and does not add to the efficacy of topical budesonide.
  •  
2.
  •  
3.
  • Barfod, Anders, et al. (författare)
  • In vitro selection of RNA aptamers against a conserved region of the Plasmodium falciparum erythrocyte membrane protein 1.
  • 2009
  • Ingår i: Parasitology Reseach. - : Springer Science and Business Media LLC. - 1432-1955 .- 0932-0113. ; Aug 20, s. 1557-1566
  • Tidskriftsartikel (refereegranskat)abstract
    • The var-gene encoding Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) is known to play a major role in the pathogenicity of the P. falciparum parasite. The protein enables the parasite to adhere to the endothelial linings of small blood vessels (cytoadherence) as well as to non-infected erythrocytes (rosetting), thus preventing clearance from the bloodstream. The development and spread of resistance towards most anti-malarial drugs used for treatment and prevention of the most severe form of malaria truly emphasise the importance of a continuous research and development of new drugs. In this study we use Systematic Evolution of Ligands by EXponential enrichment (SELEX) methodology to isolate high-affinity ligands (aptamers). To validate the results from the SELEX in vitro selection, different aptamers have been selected against PfEMP1 in a live cell assay of P. falciparum strain FCR3S1.2, a highly rosetting strain. We have been able to show the rosette disrupting capacity of these SELEX-aptamers at concentrations of 33 nM and with 100% disruption at 387 nM. The described results show that RNA aptamers are promising candidates for adjunct therapy in severe malaria.
  •  
4.
  •  
5.
  • Greiff, Lennart, et al. (författare)
  • Challenge-induced plasma exudation and mucinous secretion in human airways
  • 2005
  • Ingår i: Clinical Physiology and Functional Imaging. - 1475-0961. ; 25:4, s. 241-245
  • Tidskriftsartikel (refereegranskat)abstract
    • Secretion of mucins and exudation of plasma are distinct processes of importance to innate immunity and inflammatory disease. Yet, little is known about their relation in human airways. The objective of the present study was to use the human nasal airway to determine mucinous secretion and plasma exudation in response to common challenge agents and mediators. Ten healthy volunteers were subjected to nasal challenge-lavage procedures. Thus, the nasal mucosa was exposed to increasing doses of histamine (40 and 400 microg ml(-1)), methacholine (12.5 and 25 mg) and capsaicin (30 and 300 ng ml(-1)). Fucose was selected as a global marker of mucinous secretion and alpha(2)-macroglobulin as an index of exudation of bulk plasma. All challenge agents increased the mucosal output of fucose to about the same level (P<0.01-0.05). Once significant secretion had been induced the subsequently increased dose of the challenge agent, in the case of histamine and methacholine, failed to further increase the response. Only histamine increased the mucosal output of alpha(2)-macroglobulin (P<0.01). We conclude that prompt but potentially rapidly depleted mucinous secretion is common to different kinds of airway challenges, whereas inflammatory histamine-type mediators are required to produce plasma exudation. Along with the acknowledged secretion of mucins, a practically non-depletable, pluripotent mucosal output of plasma emerges as an important component of the innate immunity of human airways.
  •  
6.
  • Hamsten, Carl, 1981-, et al. (författare)
  • Expression and immunogenicity of six putative variable surface proteins in Mycoplasma mycoides subsp. mycoides SC.
  • 2008
  • Ingår i: Microbiology. - Reading : Society for General Microbiology. - 1350-0872 .- 1465-2080. ; 154, s. 539-549
  • Tidskriftsartikel (refereegranskat)abstract
    • Variable surface protein Vmm and five Vmm-type proteins from Mycoplasma mycoides subsp. mycoides SC were analysed to determine whether these proteins are expressed in vivo in animals affected by contagious bovine pleuropneumonia (CBPP) and in vitro. Recombinant versions of these proteins were constructed and expressed in Escherichia coli after mutation of the TGA Trp codons to TGG. These proteins were then analysed by dot and Western blotting with sera from CBPP-affected cattle. Furthermore, affinity-purified polyclonal antibodies to the recombinant proteins were used in Western and colony blotting to look for expression of the putative Vmm-type proteins in cultured M. mycoides SC. This study demonstrates that immunoglobulins in CBPP sera recognize all putative Vmm-type proteins tested, indicating that these proteins or their homologues are expressed by mycoplasmas during natural infections. Vmm and one of the putative Vmm-type proteins showed variable expression in vitro.
  •  
7.
  • Hamsten, Carl, 1981- (författare)
  • Protein based approaches to understand and prevent contagious bovine pleuropneumonia
  • 2009
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • Contagious bovine pleuropneumonia (CBPP) is a severe infectious disease caused by Mycoplasma mycoides subsp. mycoides small colony type (M. mycoides SC) and is a vast problem in Africa. Current CBPP prevention is based on attenuated live strain vaccines, but these are limited by factors such as short-term immunity, cold-chain dependence and retained virulence. CBPP can be diagnosed using post-mortem examination, identification of the agent using culture and PCR based methods as well as serological diagnostic methods, but the latter are generally not sensitive enough and there is also demand for an inexpensive, pen side field test.The research presented in this thesis was focused on using recombinantly expressed surface proteins from M. mycoides SC to characterize humoral immune responses to CBPP. Thereby candidate proteins to be used in development of serological diagnostic methods and possibly subunit vaccines could be identified. As a first step, five putative variable surface proteins of M. mycoides SC were expressed and purified from E. coli in Paper I. These proteins were analyzed using immunoblotting techniques and results showed that one protein, MSC_0364, was variably expressed on the surface of M. mycoides SC in vitro. Paper II presents expanded efforts including cloning and expression of 64 recombinant surface proteins and an assay for high throughput analysis of protein-specific IgG, IgA and IgM titers in hundreds of sera using a bead-based screening assay. The assay was evaluated by protein-specific inhibition experiments, comparisons to Western blotting and monitoring of immune responses over time in a study with sera taken from eight animals over 293 days from a previous vaccine trial.Papers III and IV present applications using the recombinant proteins and bead-based screening assay wherein proteins for diagnostic and vaccine development were identified. In Paper III, the assay was used to screen 61 proteins using well-characterized serum samples from cattle with CBPP and healthy controls, resulting in selection of eight proteins suitable for diagnostic use. These proteins were combined and evaluated in a proof-of-concept ELISA with a discriminative power that enabled 96% correct classification of sera from CBPP-affected and CBPP-free bovines. Paper IV reports the results and protein-specific analyses of a vaccine trial using the recombinant putative variable surface proteins presented in Paper I as a subunit vaccine. The vaccine conferred no protection, but a weak vaccine response could not be excluded as the cause of failure. In an effort to identity other protein candidates to be used in a subunit vaccine, protein-specific analysis of humoral immune responses elicited by the currently approved live strain vaccine, T1/44, were investigated. Here, five proteins with high IgG titers associated to immunity were identified: LppQ, MSC_02714, MSC_0136, MSC_0079 and MSC_0431. These proteins may be important in the development of a novel subunit vaccine against CBPP.
  •  
8.
  • Hamsten, Carl, 1981-, et al. (författare)
  • Recombinant surface proteomics as a tool to analyze humoral immune responses in bovines infected by Mycoplasma mycoides subsp. mycoides SC
  • 2009
  • Ingår i: Molecular & Cellular Proteomics. - Stanford : HighWire Press. - 1535-9476 .- 1535-9484. ; 8:11, s. 2544-2554
  • Tidskriftsartikel (refereegranskat)abstract
    • A systematic approach to characterize the surface proteome of Mycoplasma mycoides subspecies mycoides small colony type (M. mycoides SC), the causing agent of contagious bovine pleuropneumonia (CBPP) in cattle, is presented. Humoral immune responses in 242 CBPP affected cattle and controls were monitored against one third of the surface proteins of M. mycoides SC in a high-throughput magnetic bead based assay. First, 64 surface proteins were selected from the genome sequence of M. mycoides SC and expressed as recombinant proteins in E. coli. Binding of antibodies to each individual protein could then be analyzed simultaneously in minute sample volumes with the Luminex suspension array technology. The assay was optimized on Namibian CBPP positive sera and Swedish negative controls to allow detection and 20-fold mean signal separation between CBPP positive and negative sera. Signals were proven to be protein-specific by inhibition experiments and results agreed with western blot experiments. The assay's potential to monitor IgG, IgM and IgA responses over time was shown in a proof-of-concept study with 116 sera from 8 animals in a CBPP vaccine study. In conclusion, a toolbox with recombinant proteins and a flexible suspension array assay that allows multiplex analysis of humoral immune responses to M mycoides SC, has been created.
  •  
9.
  • Holmlund, Sofia, 1972- (författare)
  • Jorden vi ärvde : Arvsöverlåtelser och familjestrateger på den uppländska landsbygden 1810-1930
  • 2007
  • Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
    • This thesis investigates inheritance among landowning families in the parish of Estuna in east-central Sweden between 1810 and 1930. The patterns of action examined in the study are analyzed as strategies in terms of objectives and principles on the one hand, and means towards these objectives and principles on the other. Before 1885, strategies were based on family interest. The individual’s dependency on inheritance was strong: a fact manifesting itself e.g. in the strong connections between inheritance and matrimonial patterns. The principal goal of the family strategies was to accomplish a transfer of the estate under sustainable conditions to one of the heirs, preferably – but not necessarily – a son. After 1845, as a response to institutional and social change, forms of conveyance changed. For example, after the introduction of equal rights of inheritance between sons and daughters in 1846, the number of quasi-commercial sales of land directly to sons increased, as a way of circumventing judicial demands. Yet this change of action in no way counter-acted the comprehensive goals and principles of inheritance. On the contrary, it was a means to overcome new difficulties in accomplishing these goals. After 1885, inheritance strategies reflected individual, rather than collective, aims. Estates were parcelled and the lots sold by the heirs at a profit. Furthermore, matrimony no longer showed connection with the spouses’ respective inheritance. This development was a result of institutional developments as well as of economic change, both diminishing paternal power. Industrialization had created openings outside domestic agriculture, and so individuals became less dependent on family and family resources. During this period, the older generation tended to keep their estates as long as possible, and this was read as a defensive strategy aimed at continued maintenance of estates within the family.
  •  
10.
  • Howarth, Peter H, et al. (författare)
  • Objective monitoring of nasal airway inflammation in rhinitis
  • 2005
  • Ingår i: The Journal of Allergy and Clinical Immunology. - : Elsevier BV. - 1097-6825 .- 0091-6749. ; 115:3, Suppl 1, s. 414-441
  • Tidskriftsartikel (refereegranskat)abstract
    • Allergic rhinitis is an inflammatory nasal disorder in which a range of different cells participates. A variety of approaches has been used to monitor nasal inflammation objectively to investigate disease processes and to evaluate the effect of therapeutic intervention. These approaches include nasal lavage, nasal cytology, and nasal biopsy, together with the more recently established measurement of nasal nitric oxide (NO) concentration. Although all provide information about nasal mucosal inflammation, the extent of information that can be obtained by each approach, the ease of sampling, and the complexity of sample handling differ. Such considerations influence the choice of approach when measurement of nasal inflammation is to be an objective outcome parameter in a clinical trial. In addition, the choice of approach is also determined by the questions or hypotheses that are to be addressed. Nasal lavage is simple and rapid to perform, is well tolerated, and provides a sample that can provide information about luminal cell recruitment, cell activation, and plasma protein extravasation. Nasal cytology involves sampling and recovering mucosal surface cells. It is also easy to perform and is well tolerated in general, although some find that the procedure causes a transient unpleasant sensation. A differential cell count from the sample provides information about relative cell populations. Both nasal lavage and nasal cytology are readily applicable to clinical trials. Nasal cytology sample handling is easier, but nasal lavage offers the advantage of providing considerably greater information from the sample. Nasal biopsy is a considerably more invasive procedure and requires expertise not only in tissue sampling but also in biopsy processing. Therefore, it is applicable only in specialist centers. However, nasal biopsy is the only sampling technique that directly informs about tissue cellular events, although these may be implied, in part from the other sampling approaches. Tissue specimens can be used to evaluate both protein and gene expression. Measurement of nasal NO involves expensive equipment but provides an instantaneous result, unlike the other approaches, all of which require sample processing and analysis. Recommendations for standardization of measurement have been made, and measures are considered in part to reflect allergic inflammation within the nasal mucosa. The limitations of nasal NO are that it reflects only a certain aspect of allergic mucosal inflammation, and that because a proportion of nasally measured NO is derived from the sinuses under normal circumstances, nasal NO is not specific for nasal disease. The high contribution from the sinus mucosa limits the discriminatory ability of nasal NO to reflect nasal tissue-specific alterations. The incorporation of measures of nasal inflammation in clinical trials has distinguished anti-inflammatory therapy from symptomatic therapy and has the potential to provide information about the efficacy of novel therapies for allergic rhinitis.
  •  
Skapa referenser, mejla, bekava och länka
  • Resultat 1-10 av 56
Typ av publikation
tidskriftsartikel (33)
konferensbidrag (12)
doktorsavhandling (4)
forskningsöversikt (4)
licentiatavhandling (2)
bokkapitel (1)
visa fler...
visa färre...
Typ av innehåll
refereegranskat (45)
övrigt vetenskapligt/konstnärligt (10)
populärvet., debatt m.m. (1)
Författare/redaktör
Persson, Carl (19)
Greiff, Lennart (9)
Andersson, Morgan (8)
Gudowski, Waclaw (6)
Uller, Lena (6)
Borrebaeck, Carl (4)
visa fler...
Erjefält, Jonas (4)
Folke, Carl (4)
Svedin, Uno (4)
Sörlin, Sverker (4)
Wingren, Christer (4)
Rockström, Johan (4)
Steffen, Will (4)
Nihlén, Ulf (4)
Persson, Anja (4)
Ingvarsson, Johan (4)
Korsgren, Magnus (3)
Löfdahl, Claes-Göran (3)
Walker, Brian (3)
Nyberg, Per (3)
Karlberg, Louise (3)
Rodhe, Henning (3)
Persson, Åsa (3)
Sjöstedt, Carl-Johan (3)
Jönsson, Per (2)
Chapin, F. Stuart, I ... (2)
Uhlén, Mathias (2)
Schwenk, Jochen M. (2)
Sterner, Olov (2)
Lenton, Timothy M. (2)
Carlsson, Anders (2)
Salford, Leif (2)
Van Der Leeuw, Sande ... (2)
Skagerberg, Gunnar (2)
Seltborg, Per (2)
Montnémery, Peter (2)
Siesjö, Peter (2)
Alenmyr, Lisa (2)
Malm-Erjefält, Monik ... (2)
Rydell-Törmänen, Kri ... (2)
Nykvist, Björn (2)
Richardson, Katherin ... (2)
Neiman, Maja (2)
Scheffer, Marten (2)
Berglund, Magnus (2)
Skogvall, Staffan (2)
Bournos, Victor (2)
Serafimovich, Ivan (2)
Liverman, Diana (2)
Schellnhuber, Hans J ... (2)
visa färre...
Lärosäte
Lunds universitet (25)
Kungliga Tekniska Högskolan (17)
Uppsala universitet (6)
Stockholms universitet (6)
Linköpings universitet (2)
Karolinska Institutet (2)
visa fler...
Göteborgs universitet (1)
Umeå universitet (1)
Mälardalens universitet (1)
Mittuniversitetet (1)
visa färre...
Språk
Engelska (52)
Svenska (4)
Forskningsämne (UKÄ/SCB)
Medicin och hälsovetenskap (28)
Naturvetenskap (11)
Teknik (7)
Samhällsvetenskap (2)
Humaniora (2)

År

Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.

 
pil uppåt Stäng

Kopiera och spara länken för att återkomma till aktuell vy