| 11. |
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| 12. |
- Alyahya, G. A., et al.
(författare)
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Pleomorphic adenoma arising in an accessory lacrimal gland of Wolfring
- 2006
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Ingår i: Ophthalmology. - : Elsevier BV. - 0161-6420. ; 113:5, s. 879-82
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Tidskriftsartikel (refereegranskat)abstract
- PURPOSE: To describe a patient with pleomorphic adenoma arising in an accessory lacrimal gland of Wolfring in the lower lid and to illustrate the immunohistochemical and molecular cytogenetics. DESIGN: Single interventional case report. METHODS: A 62-year-old man presented with a 20-year history of a painless slowly growing mass at the temporal part of the right lower eyelid. Histological, immunohistochemical, and fluorescence in situ hybridization studies of the excised tumor were performed. RESULTS: Histological evaluation showed many glandular elements embedded in a myxoid stroma. The tumor was situated beneath an area of a normal accessory lacrimal gland of Wolfring and in close association with normal meibomian glands. Myoepithelial tumor cells in the myxoid stroma reacted strongly with an antibody against glial fibrillary acidic protein, which did not bind to normal lacrimal gland tissue. Tumor cells with both epithelial and myoepithelial morphologies reacted positively for both pleomorphic adenoma gene-1 and high-mobility group A2 proteins. Fluorescence in situ hybridization analysis showed no evidence of clonal translocations or numerical abnormalities involving chromosome 8 or 12. CONCLUSIONS: Pleomorphic adenoma of the accessory lacrimal gland is an exceedingly rare tumor of the ocular adnexa. Glial fibrillary acidic protein seems to be a tumor-associated antigen. Genetically, this case of pleomorphic adenoma arising from an accessory lacrimal gland of Wolfring is identical with those originating from salivary glands.
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| 13. |
- Asp, Julia, 1973, et al.
(författare)
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CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas.
- 2006
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Ingår i: Genes, chromosomes & cancer. - : Wiley. - 1045-2257 .- 1098-2264. ; 45:9, s. 820-8
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Tidskriftsartikel (refereegranskat)abstract
- Pleomorphic salivary gland adenomas are characterized by recurrent chromosome rearrangements of 8q12, leading to activation of the PLAG1 oncogene. Here we demonstrate that CHCHD7-PLAG1 is a novel and recurrent gene fusion generated by a cytogenetically cryptic rearrangement in pleomorphic adenomas. CHCHD7 is a newly identified member of a multifamily of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain. Northern blot analysis revealed that the gene is ubiquitously expressed. Its biological function is unknown and the gene has hitherto not been associated with neoplasia. CHCHD7 and PLAG1 are located head-to-head about 500 bp apart in 8q12. Molecular analyses of 27 tumors revealed CHCHD7-PLAG1 fusions in three tumors, two of which had t(6;8) and t(8;15) translocations as the sole anomalies and one a normal karyotype. FISH analyses of interphase nuclei and nuclear chromatin fibers of a fourth adenoma with a normal karyotype revealed that a second fusion partner gene, TCEA1, located about 2 Mb centromeric to PLAG1, also is fused to PLAG1 as a result of a cryptic 8q rearrangement. The breakpoints in both fusions occur in the 5'-noncoding regions of the genes, leading to activation of PLAG1 by promoter swapping/substitution. Western blot and immunohistochemical analyses demonstrated that the PLAG1 protein was overexpressed in epithelial, myoepithelial, and mesenchymal-like tumor cells in tumors with both fusions. Our findings further emphasize the significance of PLAG1 activation in pleomorphic adenomas and demonstrate that the gene is more frequently activated than previously anticipated.
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| 14. |
- Balaz, Martina, et al.
(författare)
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Protein-surface Interactions and Functional Geometry of Surface-adsorbed Myosin Motor Fragments
- 2009
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Ingår i: Biophysical Journal. - : Biophysical Society. - 0006-3495 .- 1542-0086. ; 96:3 Suppl. 1, s. 495A-495A
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Tidskriftsartikel (övrigt vetenskapligt/konstnärligt)abstract
- Biophysical studies with myosin motor fragments (heavy meromyosin; HMM and subfragment 1; S1) adsorbed to artificial surfaces, are important for elucidation of actomyosin function. In spite of the widespread use of such in vitro motility assays and single molecule studies, little is known about the adsorption geometry and effects of protein-surface interactions on the motor properties. Here, we investigate these factors with focus on HMM using quartz crystal microbalance with dissipation (QCM-D) and total internal reflection fluorescence (TIRF) spectroscopy based ATPase assays. In the latter, we monitored the turnover of Alexa-fluor647-ATP (Alexa-ATP) by surface adsorbed HMM. Studies were performed with HMM/S1 adsorbed to model hydrophilic (SiO2) or hydrophobic (trimethyl-chlorosilane [TMCS] - derivatized) surfaces. The results suggest that adsorption of HMM is weakened on SiO2 (but not on TMCS) at high (245 mM) compared to low (65 mM) ionic strengths. The changes in ionic strength were also associated with structural changes in the protein layer according to QCM-D studies. Moreover, the TIRF based ATPase assay suggested a larger fraction of HMM molecules with low catalytic activity on SiO2. These and other TIRF and QCM-D results, suggest that HMM preferentially adsorbs to negatively charged hydrophilic surfaces via the actin-binding region. In contrast, the majority of the HMM molecules seem to adsorb via their C-terminal tail on moderately hydrophobic surfaces. In the latter case the catalytic sites appear to be close to, but not immobilized on the surface. The results with HMM were compared to, and found consistent with, QCM-D and TIRF-data obtained with S1 motor fragments.
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| 15. |
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| 16. |
- Berglund, Lisa, et al.
(författare)
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A genecentric Human Protein Atlas for expression profiles based on antibodies
- 2008
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Ingår i: Molecular & Cellular Proteomics. - 1535-9476 .- 1535-9484. ; 7:10, s. 2019-2027
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Forskningsöversikt (refereegranskat)abstract
- An attractive path forward in proteomics is to experimentally annotate the human protein complement of the genome in a genecentric manner. Using antibodies, it might be possible to design protein-specific probes for a representative protein from every protein-coding gene and to subsequently use the antibodies for systematical analysis of cellular distribution and subcellular localization of proteins in normal and disease tissues. A new version (4.0) of the Human Protein Atlas has been developed in a genecentric manner with the inclusion of all human genes and splice variants predicted from genome efforts together with a visualization of each protein with characteristics such as predicted membrane regions, signal peptide, and protein domains and new plots showing the uniqueness (sequence similarity) of every fraction of each protein toward all other human proteins. The new version is based on tissue profiles generated from 6120 antibodies with more than five million immunohistochemistry-based images covering 5067 human genes, corresponding to approximately 25% of the human genome. Version 4.0 includes a putative list of members in various protein classes, both functional classes, such as kinases, transcription factors, G-protein-coupled receptors, etc., and project-related classes, such as candidate genes for cancer or cardiovascular diseases. The exact antigen sequence for the internally generated antibodies has also been released together with a visualization of the application-specific validation performed for each antibody, including a protein array assay, Western blot analysis, immunohistochemistry, and, for a large fraction, immunofluorescence-based confocal microscopy. New search functionalities have been added to allow complex queries regarding protein expression profiles, protein classes, and chromosome location. The new version of the protein atlas thus is a resource for many areas of biomedical research, including protein science and biomarker discovery.
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| 17. |
- Bodin, Per, et al.
(författare)
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Guidance, navigation, and control experiments on the PRISMA in-orbit test bed
- 2007
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Ingår i: 58th International Astronautical Congress, IAC-07-C1. - 9781605601502 ; , s. 4461-4470
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Konferensbidrag (refereegranskat)abstract
- PRISMA will demonstrate Guidance, Navigation and Control strategies for advanced autonomous formation flying and rendezvous. The Swedish Space Corporation (SSC) is the prime contractor for the project which is funded by the Swedish National Space Board (SNSB). The project is further supported by the German Aerospace Center (DLR), the Technical University of Denmark (DTU), and the French Space Agency (CNES). PRISMA consists of two spacecraft: MAIN and TARGET. The MAIN satellite is 3-axis stabilized and has full 3D delta-V maneuverability that is independent of the spacecraft's attitude. The TARGET satellite has a simplified, yet 3-axis stabilizing, magnetic attitude control system and no orbit maneuver capability. This paper presents the PRISMA Guidance, Navigation, and Control (GNC) subsystem. The paper gives a mission summary and an overview of the GNC subsystem with its hardware and software configuration. It also explains how the orbit control functions contain advanced fuel optimal Model Predictive Control (MPC). It is shown how the GNC software is developed using model based automatic coding technology implemented with Matlab/Simulink. The paper then summarizes the different GNC experiments to be performed by SSC. Finally, an overview of the test approach for the subsystem is given.
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| 18. |
- Carlström, Mattias, et al.
(författare)
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Role of nitric oxide deficiency in the development of hypertension in hydronephrotic animals
- 2008
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Ingår i: American Journal of Physiology - Renal Physiology. - : American Physiological Society. - 0363-6127 .- 1522-1466 .- 1931-857X. ; 294:2, s. 362-370
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Tidskriftsartikel (refereegranskat)abstract
- Hydronephrotic animals develop renal injury and hypertension, which is associated with an abnormal tubuloglomerular feedback (TGF). The TGF sensitivity is coupled to nitric oxide (NO) in the macula densa. The involvement of reduced NO availability in the development of hypertension in hydronephrosis was investigated. Hydronephrosis was induced by ureteral obstruction in young rats. Blood pressure and renal excretion were measured in adulthood, under different sodium conditions, and before and after chronic administration of either N-G- nitro-L-arginine methyl ester (L-NAME) or L-arginine. Blood samples for ADMA, SDMA, and L-arginine analysis were taken and the renal tissue was used for histology and determination of NO synthase (NOS) proteins. TGF characteristics were determined by stop-flow pressure technique before and after administration of 7-nitroindazole (7-NI) or L-arginine. Hydronephrotic animals developed salt-sensitive hypertension, which was associated with pressure natriuresis and diuresis. The blood pressure response to L-NAME was attenuated and L-arginine supplementation decreased blood pressure in hydronephrotic animals, but not in the controls. Under control conditions, reactivity and sensitivity of the TGF response were greater in the hydronephrotic group. 7-NI administration increased TGF reactivity and sensitivity in control animals, whereas, in hydronephrotic animals, neuronal NOS (nNOS) inhibition had no effect. L-Arginine attenuated TGF response more in hydronephrotic kidneys than in controls. The hydronephrotic animals displayed various degrees of histopathological changes. ADMA and SDMA levels were higher and the renal expressions of nNOS and endothelial NOS proteins were lower in animals with hydronephrosis. Reduced NO availability in the diseased kidney in hydronephrosis, and subsequent resetting of the TGF mechanism, plays an important role in the development of hypertension.
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| 19. |
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Den dubbla blicken : Historia i de nordiska samhällena krings sekelskiftet 1900
- 2007
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Samlingsverk (redaktörskap) (övrigt vetenskapligt/konstnärligt)abstract
- År 1905 arrangerades det första nordiska historikermötet i Lund. Mötet blev till viss del ett misslyckande eftersom norrmännen uteblev på grund av den svensk-norska unionskrisen. Några få finländare medverkade - men de hade sin politiska och historieteoretiska uppmärksamhet riktad åt andra håll. De nordatlantiska samhällena hade ännu inga akademiska historiker. Mötets öde visar hur historia och historievetenskap alltid existerar i en samtida samhällelig kontext. I denna bok, resultatet av en jubileumskonferens i Lund år 2005, studeras historiebruket i de nordiska samhällena för hundra år sedan av forskare från Danmark, Finland, Grönland, Island, Norge och Finland. Historien användes på många sätt, av norrländska hembygdsentusiaster, norska professorer och grönländska skollärare. Gemensamt för dem alla var den starka kopplingen till naitonalismen, som såg olika ut i de olika nordiska länderna. Sättet att förhålla sig till historien var dubbelt. Från nuet riktades blicken mot både det förflutna och framtiden.
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| 20. |
- Edelvik, Fredrik, 1972, et al.
(författare)
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An improved method for dipole modeling in EEG-based source localization
- 2009
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Ingår i: International Federation for Medical and Biological Engineering Proceedings. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 1680-0737. - 9783642038884 ; 25:9, s. 146-149
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Konferensbidrag (refereegranskat)abstract
- The inverse problem in EEG-based source localizationis to determine the location of the brain sources that areresponsible for the measured potentials at the scalp electrodes.The brain sources are usually modeled as current dipoles whichlead to a singularity in the right-hand side of the governing Poisson’sequation. Subtraction methods have been proposed as aremedy and in this paper an improved subtraction method formodeling the dipoles is presented. The accuracy is demonstratedfor radial and tangential sources in layered sphere models and isto the best of the authors’ knowledge superior to previous methodsfor superficial sources. An additional advantage is that itproduces a right hand side with few non-zeros which is beneficialfor efficient solution of the inverse problem.
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