| 1. |
- Aguda, Adeleke H., 1969-
(författare)
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Structural Study of the WH2 Family and Filamin: Implications for Actin Cytoskeleton Regulation
- 2006
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Cellular processes like motility, chemotaxis, phagocytosis and morphogenesis are dependent on the dynamic regulation of the actin cytoskeleton. This cytoskeleton system is tightly controlled by a number of diverse actin-binding proteins (ABPs) by various mechanisms described as nucleation, polymerization, capping, severing, depolymerization and sequestration. The ABPs are grouped based on sequence identity as in the Wiskott-Aldrich Syndrome protein homology domain 2 (WH2), and the calponin homology domain (CH) containing proteins.In this work, we elucidate the crystal structures of hybrids of gelsolin domain 1 with thymosin β4, ciboulot domain 2, and the second WH2 domain of N-WASP each bound to actin. We show that the single WH2 motif containing protein thymosin β4 in part sequesters actin by binding its pointed end via a C-terminal helix. This interaction prevents the addition of bound actin protomers to the barbed end of the filament. We propose that sequence variations in some WH2 motifs conferred F-actin binding ability to multiple repeat-containing proteins. These F-actin binding domains interact with the barbed end of a filament and the adjacent WH2 motifs are then freed to add their bound actin to the growing filament end. We demonstrate the binding of ciboulot domains 2 and 3 to both G- and F-actin and that full length ciboulot is capable of binding two actin monomers simultaneously. We have also cloned, expressed, purified and crystallized rod domains 14-16 from the actin crosslinking protein a-filamin. Preliminary X-ray crystallography data gives us hope that we shall be able to solve the structure of this triple domain repeat.
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| 2. |
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| 3. |
- Stehn, Lars, et al.
(författare)
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Byggandet av flervåningshus i trä : erfarenhet efter tre års observation av träbyggandets utveckling
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- I utredningen ”Mer trä i byggandet”, Ds 2004:01, som ledde fram till Nationella träbyggnadsstrategin slogs fast att: för att öka träbyggandet krävs utbildningsinsatser både av studenter och via fortbildning av yrkesverksamma ute i arbetslivet. Denna rapport är en sammanfattning av kunskaperna vunna ur de aktiviteter som genomfördes inom Nationella träbyggnadsstrategins fortbildningsprogram mellan 2006-2008. Kunskaper och resultat har även vävts in från flera FoU program riktat att stötta träbyggandet som utfördes parallellt med Nationella träbyggnadsstrategin. Fortbildningsprogrammet var ett samverkansprojekt mellanLuleå tekniska universitet, Växjö universitet, Högskolan Dalarna och SP Trätek I rapporten beskrivs: Teknik och kompetensutveckling inom träbyggandet, Systembeskrivningar av träbyggprojekt med fokus på objekt uppförda i Växjö, Falun och Skellefteå. Vidare ges vetenskapliga systembeskrivningar av industriellt träbyggande med definitioner byggsystem (tekniken), av aktörsroller, affärsmodeller och vad egentligen industriellt byggande handlar om (processen för industriellt byggande). Rapporten avslutas med en vägledning till byggandets aktörer: kommuner, arkitekter, leverantörer/entreprenörer.
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| 4. |
- Stehn, Lars, et al.
(författare)
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Byggandet av flervåningshus i trä : Ett samverkansprojekt inom Nationella träbyggnadsstrategins fortbildningsprogram
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- Denna rapport är en sammanfattning och en analys av kunskaperna vunna ur de aktiviteter som genomfördes inom Nationella träbyggnadsstrategins fortbildningsprogram mellan 2006 - 2008. Kunskaper och resultat har även vävts in från flera FoU program, riktat att stötta träbyggandet, som utfördes parallellt med Nationella träbyggnadsstrategin.Fortbildningsprogrammet var ett samverkansprojekt mellan: Luleå tekniska universitet, Växjö universitet, Högskolan Dalarna och SP Trätek.Rapportens olika delar är författad av skilda författare från samverkansparterna. I rapporten beskrivs:Den byggteknik- och kompetensutveckling som har skett inom träbyggandet.• I dag finns det tre typer av träbyggnadssystem (pelar-balk system, skivsystem av massivträ och regelsystem) som kan kombineras i tre olika byggmetoder (platsbygge, prefabricerade ytelement och prefabricerade volymer).• Under programmets gång genomfördes seminarier och kunskapsfördjupande workshops till uppskattningsvis 750 personer som representerade beställare/förvaltare, arkitekter, byggkonsulter och byggentreprenörer. Förutom de 15 byggprojekten som djupdokumenterats (se kapitel 3) så är bedömningen att ett tio-tal träbyggprojekt initierades som en följd av aktiviteterna inom fortbildningsprogrammet.Man kan också konstatera att fortbildningsprogrammet stöttade den pågående bildningen av starka akademiska miljöer med fokus på träbyggande.Systembeskrivningar av träbyggprojekt med fokus på de byggobjekten som uppfördes i Växjö, Falun och Skellefteå.• Detaljerade redogörelser beskriver: Planering, projektering och upphandling, byggnadsutformning, lösningar för brand, akustik och stabilisering. Detaljer för konstruktionselement (väggar och bjälklag) och erfarenheter från montage och logistik och förslag på förbättringsåtgärder.Vetenskapliga systembeskrivningar av industriellt träbyggande.• En karakterisering presenteras av byggsystemen (tekniken).• En beskrivning och analys av den industriella flerbostadsbranschens framväxt och potential samt dess lönsamhet.• Analyser av aktörsroller, affärsmodeller och definitionen av industriellt byggande (processen för industriellt byggande).Rapporten avslutas med en vägledning till byggandets aktörer: kommuner, arkitekter, leverantörer/entreprenörer.
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| 5. |
- Stehn, Lars, et al.
(författare)
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Byggandet av flervåningshus i trä : erfarenheter efter tre års observation av träbyggandets utveckling
- 2008
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- This report is a summary and analysis of the knowledge gained from the activities carried out 2006 - 2008 within "Nationella träbyggnadsstrategins fortbildningsprogram". Expert knowledge and results are also included from the many corresponding R&D programs, aimed to support and develop timber housing construction, which was run parallel to "Nationella träbyggnadsstrategin"."Fortbildningsprogrammet" - the program for further education of actors in the housing industry was a cooperative effort between: Luleå university of technology, Vaxjö university, Dalarna university and the institute SP Trätek.The chapters of the report are written by different authors from the universities and institute.The report describes: The building technique and competence development that have taken place within the Swedish timber housing industry (viewed in a wide context). - Three types of structural systems exist on the market today (column-beam systems, massive wood elements and timber frame systems). These are combined and erected using three different types of construction methods (on-site construction, prefabricated elements and prefabricated volume elements).- During the program, seminars, workshops and study tours were attended by approximately 750 people representing construction clients, architects, designers and construction companies. In depth case results are presented and analysed from 15 different building projects that give valuable information for other projects and it is also estimated that more than 10 other projects was initiated as an effect of the program.As an important by-effect, it is noted that the undertaking and existence of the program gave additional support to the development of research groups with focus on timber housing construction.In-depth case descriptions of timber house projects with a focus on those in Växjö, Falun och Skellefteå. - Detailed descriptions and analyses regarding: Design aspects, architectural limitations, descriptions of technical solutions for adequate fire, acoustics and horizontal stabilising. Details for several structural elements are presented and experience and further development of the logistics and the montage employed are analysed.- Detailed descriptions and analyses regarding: Design aspects, architectural limitations, descriptions of technical solutions for adequate fire, acoustics and horizontal stabilising. Details for several structural elements are presented and experience and further development of the logistics and the montage employed are analysed. Scientifically based systems descriptions of industrialised timber housing.- A characterisation of the building technique is presented. - A description and analysis of the growth and potential of the multi-storey industrialised housing industry is given.- Analysis of different actor's roles, business models including a definition of industrialised housing (the process) is displayed.The report concludes with guidance instructions aimed for all actors involved in a housing project.
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| 6. |
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| 7. |
- Aguda, Adeleke Halilu, et al.
(författare)
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Expression, crystallization and preliminary crystallographic data analysis of filamin A repeats 14-16
- 2007
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Ingår i: Acta Crystallographica. Section F. - 1744-3091 .- 1744-3091. ; 63:4, s. 291-293
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Tidskriftsartikel (refereegranskat)abstract
- Human filamin A is a 280 kDa protein involved in actin-filament cross-linking. It is structurally divided into an actin-binding headpiece (ABD) and a rod domain containing 24 immunoglobulin-like (Ig) repeats. A fragment of human filamin A (Ig repeats 14-16) was cloned and expressed in Escherichia coli and the purified protein was crystallized in 1.6 M ammonium sulfate, 2% PEG 1000 and 100 mM HEPES pH 7.5. The crystals diffracted to 1.95 A and belong to space group P2(1)2(1)2(1), with unit-cell parameters a = 50.63, b = 52.10, c = 98.46 A, alpha = beta = gamma = 90 degrees.
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| 8. |
- Andersson, Derek, et al.
(författare)
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Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties
- 2009
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Ingår i: Phytochemistry. - : Elsevier BV. - 0031-9422 .- 1873-3700. ; 70:11-12, s. 1345-1354
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Tidskriftsartikel (refereegranskat)abstract
- Myrosinases (EC 3.2.1.147) are beta-thioglucoside glucosidases present in Brassicaceae plants. These enzymes serve to protect plants against pathogens and insect pests by initiating breakdown of the secondary metabolites glucosinolates into toxic products. Several forms of myrosinases are present in plants but the properties and role of different isoenzymes are not well understood. The dicot plant model organism Arabidopsis thaliana seems to contain six myrosinase genes (TGG1-TGG6). In order to compare the different myrosinases, cDNAs corresponding to TGG1 from leaves and TGG4 and TGG5 from roots were cloned and overexpressed in Pichia pastoris. The His-tagged recombinant proteins were purified using affinity chromatography and the preparations were homogenous according to SDS-PAGE analysis. Myrosinase activity was confirmed for all forms and compared with respect to catalytic activity towards the allyl-glucosinolate sinigrin. There was a 22-fold difference in basal activity among the myrosinases. The enzymes were active in a broad pH range, are rather thermostable and active in a wide range of salt concentrations but sensitive to high salt concentrations. The myrosinases showed different activation-inhibition responses towards ascorbic acid with maximal activity around 0.7-1 mM. No activity was registered towards desulphosinigrin and this compound did not inhibit myrosinase activity towards sinigrin. All myrosinases also displayed O-beta-glucosidase activity, although with lower efficiency compared to the myrosinase activity. The differences in catalytic properties among myrosinase isozymes for function in planta are discussed.
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| 9. |
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| 10. |
- Guerriera, Gea, et al.
(författare)
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The RY/Sph element mediates transcriptional repression of MAT genes from late maturation to early seedling growth
- 2009
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Ingår i: New Phytologist. - : Wiley. - 0028-646X .- 1469-8137. ; 184:3, s. 552-565
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Tidskriftsartikel (refereegranskat)abstract
- P>In orthodox seeds, the transcriptional activator ABI3 regulates two major stages in embryo maturation: a mid-maturation (MAT) stage leading to accumulation of storage compounds, and a late maturation (LEA) stage leading to quiescence and desiccation tolerance. Our aim was to elucidate mechanisms for transcriptional shutdown of MAT genes during late maturation, to better understand phase transition between MAT and LEA stages. Using transgenic and transient approaches in Nicotiana, we examined activities of two ABI3-dependent reporter genes driven by multimeric RY and abscisic acid response elements (ABREs) from a Brassica napus napin gene, termed RY and ABRE, where the RY reporter requires ABI3 DNA binding. Expression of RY peaks during mid-maturation and drops during late maturation, mimicking the MAT gene program, and in Arabidopsis thaliana RY elements are over-represented in MAT, but not in LEA, genes. The ABI3 transactivation of RY is inhibited by staurosporine, by a PP2C phosphatase, and by a repressor of maturation genes, VAL1/HSI2. The RY element mediates repression of MAT genes, and we propose that transcriptional shutdown of the MAT program during late maturation involves inhibition of ABI3 DNA binding by dephosphorylation. Later, during seedling growth, VAL1/HSI2 family repressors silence MAT genes by binding RY elements.
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