| 31. |
- Iraola, Aitor, et al.
(författare)
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Microtomography-based Inter-Granular Network for the simulation of radionuclide diffusion and sorption in a granitic rock
- 2017
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Ingår i: Journal of Contaminant Hydrology. - : Elsevier BV. - 0169-7722 .- 1873-6009. ; 207, s. 8-16
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Tidskriftsartikel (refereegranskat)abstract
- Field investigation studies, conducted in the context of safety analyses of deep geological repositories for nuclear waste, have pointed out that in fractured crystalline rocks sorbing radionuclides can diffuse surprisingly long distances deep into the intact rock matrix; i.e. much longer distances than those predicted by reactive transport models based on a homogeneous description of the properties of the rock matrix. Here, we focus on cesium diffusion and use detailed micro characterisation data, based on micro computed tomography, along with a grain-scale Inter-Granular Network model, to offer a plausible explanation for the anomalously long cesium penetration profiles observed in these in-situ experiments. The sparse distribution of chemically reactive grains (i.e. grains belonging to sorbing mineral phases) is shown to have a strong control on the diffusive patterns of sorbing radionuclides. The computed penetration profiles of cesium agree well with an analytical model based on two parallel diffusive pathways. This agreement, along with visual inspection of the spatial distribution of cesium concentration, indicates that for sorbing radionuclides the medium indeed behaves as a composite system, with most of the mass being retained close to the injection boundary and a non-negligible part diffusing faster along preferential diffusive pathways.
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| 32. |
- Johansson, Lars-Olof, et al.
(författare)
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A Boundary Practice Perspective on Co-creation of ICT Innovations
- 2016
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Ingår i: Nordic Contributions in IS Research. - Cham : Springer Publishing Company. - 9783319435961 - 9783319435978 ; , s. 100-115
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Konferensbidrag (refereegranskat)abstract
- Research has shown that collaboration and co-creation among different groups of stakeholders add complexity and challenges to the innovation process. In this paper a study of co-creation in a multi-stakeholder innovation process is presented. The co-creation is explored and described from a boundary practice perspective. The empirical data presented in the study is based on a user-centric innovation project, Free2Ride, where researchers, developers and members of two equestrian clubs co-created a piece of ICT safety equipment consisting of a transmitter (on the horse) and a receiver (application on a smartphone) to be used by equestrian club members during their everyday riding activities. Three episodes were extracted from the empirical data and presented in the paper. From these episodes the researchers have identified four characteristics of the spanning of boundaries in co-creation from a boundary practice perspective. One of the contributions in the papers is a description of boundary practice-spanning. The research approach adopted in the study is the action case approach.
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| 33. |
- Jonasson, Pall, et al.
(författare)
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The morphologic characteristics and range of motion in the hips of athletes and non-athletes
- 2016
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Ingår i: Journal of Hip Preservation Surgery. - : Oxford University Press (OUP). - 2054-8397. ; 3:4, s. 325-332
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Tidskriftsartikel (refereegranskat)abstract
- The cam deformity may cause impingement and probably leads to osteoarthritis of the hip. The aetiology of the cam deformity is incompletely understood. Vigorous training during skeletal growth can lead to the development of cam and symptoms of femoro-acetabular impingement and subsequent osteoarthritis of the hip. The purpose of this study was to compare the radiographic characteristics and range of motion between a group of athletes and a non-athletic control group. Thirty-two male athletes (17 soccer players and 15 ice-hockey players) and thirty non-athletes, used as a control group, were examined clinically and radiographically. Hip range of motion was measured and the FADIR and FABER tests were performed. Standard radiographs of both hips were taken. The centre-edge angle, alpha angle, caput-collum-diaphysis angle, head-neck offset and Tonnis grade were registered. The athletes had a higher Tonnis grade (right P = 0.009, left P = 0.004), more pain on the FADIR test (right P = 0.006, left P = 0.001) and lower ROM in internal (right P = 0.003, left P = 0.025) and external rotation (P < 0.001). A superiorly placed cam deformity (seen on an AP pelvis view) was correlated with reduced external rotation (right P = 0.001, left P = 0.004) and mild osteoarthritis (Tonnis grade 1), (P = 0.015, left P = 0.020), while a more anteriorly placed cam deformity (seen on a modified Lauenstein view) was correlated with reduced internal rotation (right P = 0.029, left P = 0.013). A lower range of motion, more osteoarthritic changes and more pain were found in the athletes than the controls. The control group had more cam deformities than previously reported.
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| 34. |
- Kamran, Faisal, et al.
(författare)
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Transmission Near-Infrared (NIR) and Photon Time-of-Flight (PTOF) Spectroscopy in a Comparative Analysis of Pharmaceuticals
- 2015
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Ingår i: Applied Spectroscopy. - : SAGE Publications. - 1943-3530 .- 0003-7028. ; 69:3, s. 389-397
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Tidskriftsartikel (refereegranskat)abstract
- We present a comprehensive study of the application of photon time-of-flight spectroscopy (PTOFS) in the wavelength range 1050 1350 nm as a spectroscopic technique for the evaluation of the chemical composition and structural properties of pharmaceutical tablets. PTOFS is compared to transmission near-infrared spectroscopy (NIRS). In contrast to transmission NIRS, PTOFS is capable of directly and independently determining the absorption and reduced scattering coefficients of the medium. Chemometric models were built on the evaluated absorption spectra for predicting tablet drug concentration. Results are compared to corresponding predictions built on transmission NIRS measurements. The predictive ability of PTOFS and transmission NIRS is comparable when models are based on uniformly distributed tablet sets. For non-uniform distribution of tablets based on particle sizes, the prediction ability of PTOFS is better than that of transmission NIRS. Analysis of reduced scattering spectra shows that PTOFS is able to characterize tablet microstructure and manufacturing process parameters. In contrast to the chemometric pseudovariables provided by transmission NIRS, PTOFS provides physically meaningful quantities such as scattering strength and slope of particle size. The ability of PTOFS to quantify the reduced scattering spectra, together with its robustness in predicting drug content, makes it suitable for such evaluations in the pharmaceutical industry.
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| 35. |
- Karatsivos, Evripidis, et al.
(författare)
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Disturbance Management in a DC Grid using a Hierarchical Control Structure
- 2015
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Ingår i: Lund Symposium 27/28 May, 2015.
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Konferensbidrag (refereegranskat)abstract
- DC grids will play a key role in the future, interconnecting generation and load centers as well as energy markets. Large multi-terminal VSC-HVDC systems of high capacity will require automated coordination for several operation scenarios especially when operating under disturbances. With this in mind, and considering the dynamics of a DC system, communication loss events are considered severe disturbances in the operation of the multi-terminal system along with major electrical faults such as 3-phase faults on the AC sides of the converters that require considerable coordination efforts. This work aims at enabling the multi-terminal system with communication loss ride-through and automatic post-fault rescheduling capabilities. This is achieved within a hierarchical control structure whose features are briefly described. A 3-terminal VSC-HVDC system is modelled for the needs of demonstration under different operation scenarios. Communication loss ride-through capability is demonstrated by comparing the behavior of the 3-terminal system during a normal operation scenario and a worst-case scenario where communications with all-three terminals fail. Its behavior does not change but since communications fail, the operation is uncoordinated greatly reducing the security of the system regarding upcoming events. During 3-phase faults DC droop performs the primary control stabilizing the system. Secondary control is performed by the overall control after the system state is identified. Automatic, post-fault rescheduling takes place in an effort to follow the power flow schedule where possible without exceeding substation limits.
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| 36. |
- Karbalaei, Mardjaneh, et al.
(författare)
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Detrusor Induction of miR-132/212 following Bladder Outlet Obstruction: Association with MeCP2 Repression and Cell Viability.
- 2015
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 10:1
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Tidskriftsartikel (refereegranskat)abstract
- The microRNAs (miRNAs) miR-132 and miR-212 have been found to regulate synaptic plasticity and cholinergic signaling and recent work has demonstrated roles outside of the CNS, including in smooth muscle. Here, we examined if miR-132 and miR-212 are induced in the urinary bladder following outlet obstruction and whether this correlates with effects on gene expression and cell growth. Three to seven-fold induction of miR-132/212 was found at 10 days of obstruction and this was selective for the detrusor layer. We cross-referenced putative binding sites in the miR-132/212 promoter with transcription factors that were predicted to be active in the obstruction model. This suggested involvement of Creb and Ahr in miR-132/212 induction. Creb phosphorylation (S-133) was not increased, but the number of Ahr positive nuclei increased. Moreover, we found that serum stimulation and protein kinase C activation induced miR-132/212 in human detrusor cells. To identify miR-132/212 targets, we correlated the mRNA levels of validated targets with the miRNA levels. Significant correlations between miR-132/212 and MeCP2, Ep300, Pnkd and Jarid1a were observed, and the protein levels of MeCP2, Pnkd and Ache were reduced after obstruction. Reduction of Ache however closely matched a 90% reduction of synapse density arguing that its repression was unrelated to miR-132/212 induction. Importantly, transfection of antimirs and mimics in cultured detrusor cells increased and decreased, respectively, the number of cells and led to changes in MeCP2 expression. In all, these findings show that obstruction of the urethra increases miR-132 and miR-212 in the detrusor and suggests that this influences gene expression and limits cell growth.
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| 37. |
- Ludvigsson, Jonas F., 1969-, et al.
(författare)
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Maternal Glycemic Control in Type 1 Diabetes and the Risk for Preterm Birth : A Population-Based Cohort Study
- 2019
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Ingår i: Annals of Internal Medicine. - : American College of Physicians. - 0003-4819 .- 1539-3704. ; 170:10, s. 691-701
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Tidskriftsartikel (refereegranskat)abstract
- Background: Maternal type 1 diabetes (T1D) has been linked to preterm birth and other adverse pregnancy outcomes. How these risks vary with glycated hemoglobin (or hemoglobin A(1c) [HbA(1c)]) levels is unclear.Objective: To examine preterm birth risk according to periconceptional HbA(1c) levels in women with T1D.Design: Population-based cohort study.Setting: Sweden, 2003 to 2014.Patients: 2474 singletons born to women with T1D and 1 165 216 reference infants born to women without diabetes.Measurements: Risk for preterm birth (< 37 gestational weeks). Secondary outcomes were neonatal death, large for gestational age, macrosomia, infant birth injury, hypoglycemia, respiratory distress, 5-minute Apgar score less than 7, and stillbirth. Results: Preterm birth occurred in 552 (22.3%) of 2474 infants born to mothers with T1D versus 54 287 (4.7%) in 1 165 216 infants born to mothers without diabetes. The incidence of preterm birth was 13.2% in women with a periconceptional HbA(1c) level below 6.5% (adjusted risk ratio [aRR] vs. women without T1D, 2.83 [95% CI, 2.28 to 3.52]), 20.6% in those with a level from 6.5% to less than 7.8% (aRR, 4.22 [CI, 3.74 to 4.75]), 28.3% in those with a level from 7.8% to less than 9.1% (aRR, 5.56 [CI, 4.84 to 6.38]), and 37.5% in those with a level of 9.1% or higher (aRR, 6.91 [CI, 5.85 to 8.17]). The corresponding aRRs for medically indicated preterm birth (n = 320) were 5.26 (CI, 3.83 to 7.22), 7.42 (CI, 6.21 to 8.86), 11.75 (CI, 9.72 to 14.20), and 17.51 (CI, 14.14 to 21.69), respectively. The corresponding aRRs for spontaneous preterm birth (n = 223) were 1.81 (CI, 1.31 to 2.52), 2.86 (CI, 2.38 to 3.44), 2.88 (CI, 2.23 to 3.71), and 2.80 (CI, 1.94 to 4.03), respectively. Increasing HbA(1c) levels were associated with the study's secondary outcomes: large for gestational age, hypoglycemia, respiratory distress, low Apgar score, neonatal death, and stillbirth.Limitation: Because HbA(1c) levels were registered annually at routine visits, they were not available for all pregnant women with T1D.Conclusion: The risk for preterm birth was strongly linked to periconceptional HbA(1c) levels. Women with HbA(1c) levels consistent with recommended target levels also were at increased risk. Primary Funding Source: Swedish Diabetes Foundation.
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| 38. |
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| 39. |
- Malehmir, Alireza, et al.
(författare)
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Planning of urban underground infrastructure using a broadband seismic landstreamer - Tomography results and uncertainty quantifications from a case study in southwestern Sweden
- 2015
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Ingår i: Geophysics. - 0016-8033 .- 1942-2156. ; 80:6, s. B177-B192
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Tidskriftsartikel (refereegranskat)abstract
- We have developed a multicomponent broadband seismic landstreamer system based on digital sensors and particularly suitable for noisy environments and areas in which high-resolution images of the subsurface are desired. We have evaluated results, interpretations, and approaches using the streamer in the planning of an approximately 3-km-long underground tunnel in the city of Varberg in the southwestern Sweden. Prospective targets were imaging of the shallow (<20 m) bedrock surface and weak zones, such as fracture and shear zones. Over the course of three weeks, 25 profiles were acquired with a total length of approximately 7.5 km using a source and receiver spacing of 2-4 m. A novel approach of the data acquisition was to integrate the landstreamer with wireless sensors in areas in which the accessibility was restricted by roads and also to increase the source-receiver distances (offsets). Although the area was highly noisy, the seismic data, in conjunction with available boreholes, successfully led to delineation of the bedrock surface, its undulations, and areas of poor rock quality. To overcome challenges due to geologic complexities and crooked-line data acquisition, 3D tomographic inversion of first breaks was carried out. Comparisons of the results with the existing boreholes indicated that in most places, the bedrock surface was well resolved by the method, which supported the indication of weak zones in the bedrock, represented by low-velocity structures in the tomographic results. We also evaluated the effect of poor geodetic surveying, particularly regarding elevation data, which adulterated the tomography results toward undulating bedrock surfaces or zones of low velocities.
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| 40. |
- Mossberg, Karin, 1981, et al.
(författare)
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Normalization of qPCR in platelets – YWHAE a potential genericreference gene
- 2016
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Ingår i: Platelets. - : Informa UK Limited. - 0953-7104 .- 1369-1635. ; 27:8, s. 729-734
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Tidskriftsartikel (refereegranskat)abstract
- The mRNA of human platelets has been extensively studied and it is generally appreciated that platelets contain mRNA transcripts derived from the megakaryocytes, and they have the ability to translate it into proteins. Additionally, platelets contain microRNA (miRNA) that has been shown to potentially regulate the translation of certain proteins. When quantifying gene expression by quantitative real-time polymerase chain reaction (qPCR), a valid normalization method is required and the use of reference genes is a common and robust approach. It is recommended to perform a proper validation of potential reference genes for each individual experimental setup. Previous studies have mainly been performed using commonly used reference genes for nucleated cells, and to our knowledge there are no global evaluations of the stability of transcripts in platelets. Finding a stable transcript would be valuable for inter-study comparisons, and the aim of this study was to identify one or more stable mRNA transcripts suitable as generic reference genes for mRNA gene expression studies in platelets. Platelets were incubated for 24 h and microarray of platelet mRNA revealed that the levels of YWHAE, B2M, ITM2B, H3F3A, PF4V1 remained similar between 0 and 24 h. Further validation of the stability of these genes together with GAPDH, RN18S1, and PPIA, genes frequently used as reference genes in platelet studies, was performed using qPCR after different in vitro conditions. In addition, inter-individual stability of the genes was analyzed in diabetic patients compared with healthy matched controls. Analysis of gene stability by the software RefFinder revealed that YWHAE, PF4V1, and B2M were the most stable genes in platelets from healthy donors. In addition, YWHAE was stable between subjects. Furthermore, the potential influence of miRNA on the selected genes was investigated by knockdown of Dicer1 in the megakaryocytic cell line MEG01. YWHAE, H3F3A, B2M, and GAPDH remained unchanged over time in MEG01 cells indicating that these genes are not regulated by miRNA and hence are more stably expressed. In conclusion, YWHAE is a stable transcript in platelets and we suggest the use of YWHAE as a generic reference gene in mRNA gene expression studies.
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