| 1. |
- Birrell, Duncan, et al.
(författare)
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Shaman : D14.2 - report on demonstration and evaluation activity in the domain of "memory institututions"
- 2011
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Rapport (övrigt vetenskapligt/konstnärligt)abstract
- 1. The report describes the demonstration and evaluation of ISP1, which was designed to demonstrate the potential of the SHAMAN framework for digital preservation in the context of memory institutions and for the research and development community. 2. The demonstration process was carried out by means of presentations based on screen-casts in three locations, Frankfurt, Vilnius and Glasgow. The audiences for the demonstrations consisted persons occupying of a wide range of roles in memory institutions, including senior management, operational level staff and IT support staff. 3. The evaluation is based on the reports of focus groups held in the three locations, together with structured data from self-completed questionnaires, administered on the same occasions. 4. Participants in the focus groups responded favourably to the ideas demonstrated in the presentations. There was particular interest in the choice of mainly open source software and in automation of processes, both of which have cost reduction implications, and in the idea of a digital preservation policy: the majority of participating organizations had no such policy. Participants also drew attention to aspects of preservation which they found lacking in the presentation and which were desirable, specifically: the preservation of font information; working with already obsolete formats; the automatic extraction of necessary metadata; the fact of mixed media archives involving, e.g., film and audio files; support for controlled vocabularies for search and discovery; and demonstration of workflows at a more practical level. 5. The questionnaire results revealed most approval of the retrieval and verification capabilities and less for the ingest processes. Otherwise the results supported the findings from the focus groups in general. There was a division of opinion over the value of the Multivalent browser and the application of grid technology, possibly because of differences in knowledge of these matters. Highest priority was assigned to data migration, access and authentication and bit stream preservation and least to independence standards and search capacity – issues that may be worth further exploration. 6. Evaluation has also been performed to determine the project‟s impact on the R&D community by means of submission and rejection rates of papers to journals and conferences, and bibliometric and Webometric analyses. The results demonstrate that the research outputs from the project are of interest to the R&D community and that the impact of the project as a whole compares favourably with other European projects in the digital preservation area. 7. The evaluation has revealed strengths and shortcomings in the demonstration process, which will influence the development of demonstrators for ISP2 and ISP3. The SHAMAN framework for digital preservation is seen as offering new possibilities and rigorous methods for the field by the practitioners in memory institutions.
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| 2. |
- Presneau, Nadege, et al.
(författare)
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Role of the transcription factor T (brachyury) in the pathogenesis of sporadic chordoma: a genetic and functional-based study
- 2011
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Ingår i: Journal of Pathology. - : Wiley. - 0022-3417. ; 223:3, s. 327-335
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Tidskriftsartikel (refereegranskat)abstract
- A variety of analyses, including fluorescence in situ hybridization (FISH), quantitative PCR (qPCR) and array CGH (aCGH), have been performed on a series of chordomas from 181 patients. Twelve of 181 (7%) tumours displayed amplification of the T locus and an additional two cases showed focal amplification; 70/181 (39%) tumours were polysomic for chromosome 6, and 8/181 (4.5%) primary tumours showed a minor allelic gain of T as assessed by FISH. No germline alteration of the T locus was identified in non-neoplastic tissue from 40 patients. Copy number gain of T was seen in a similar percentage of sacrococcygeal, mobile spine and base of skull tumours. Knockdown of T in the cell line, U-CH1, which showed polysomy of chromosome 6 involving 6q27, resulted in a marked decrease in cell proliferation and morphological features consistent with a senescence-like phenotype. The U-CH1 cell line was validated as representing chordoma by the generation of xenografts, which showed typical chordoma morphology and immunohistochemistry in the NOD/SCID/interleukin 2 receptor [IL2r]gamma(null) mouse model. In conclusion, chromosomal aberrations resulting in gain of the T locus are common in sporadic chordomas and expression of this gene is critical for proliferation of chordoma cells in vitro. Copyright (C) 2010 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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| 3. |
- Xie, Liang, et al.
(författare)
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Counterbalancing angiogenic regulatory factors control the rate of cancer progression and survival in a stage-specific manner
- 2011
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Ingår i: Proceedings of the National Academy of Sciences of the United States of America. - : Proceedings of the National Academy of Sciences. - 0027-8424 .- 1091-6490. ; 108:24, s. 9939-9944
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Tidskriftsartikel (refereegranskat)abstract
- Whereas the roles of proangiogenic factors in carcinogenesis are well established, those of endogenous angiogenesis inhibitors (EAIs) remain to be fully elaborated. We investigated the roles of three EAIs during de novo tumorigenesis to further test the angiogenic balance hypothesis, which suggests that blood vessel development in the tumor microenvironment can be governed by a net loss of negative regulators of angiogenesis in addition to the well-established principle of up-regulated angiogenesis inducers. In a mouse model of pancreatic neuroendocrine cancer, administration of endostatin, thrombospondin-1, and tumstatin peptides, as well as deletion of their genes, reveal neoplastic stage-specific effects on angiogenesis, tumor progression, and survival, correlating with endothelial expression of their receptors. Deletion of tumstatin and thrombospondin-1 in mice lacking the p53 tumor suppressor gene leads to increased incidence and reduced latency of angiogenic lymphomas associated with diminished overall survival. The results demonstrate that EAIs are part of a balance mechanism regulating tumor angiogenesis, serving as intrinsic microenvironmental barriers to tumorigenesis.
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