| 1. |
- Beal, Jacob, et al.
(författare)
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Robust estimation of bacterial cell count from optical density
- 2020
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Ingår i: Communications Biology. - : Springer Science and Business Media LLC. - 2399-3642. ; 3:1
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Tidskriftsartikel (refereegranskat)abstract
- Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressing E. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.
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| 2. |
- Kattge, Jens, et al.
(författare)
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TRY plant trait database - enhanced coverage and open access
- 2020
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Ingår i: Global Change Biology. - : Wiley-Blackwell. - 1354-1013 .- 1365-2486. ; 26:1, s. 119-188
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Tidskriftsartikel (refereegranskat)abstract
- Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives.
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| 3. |
- Abenoza, Roberto F., et al.
(författare)
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User experiences and perceptions of women-only transport services in Mexico
- 2020
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Ingår i: Gendering Smart Mobilities. - : Taylor and Francis. ; , s. 188-209
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Personal safety perceptions impact upon not only travellers’ behaviour and satisfaction but may also diminish their general well-being and health. These effects are particularly negative among female travellers, a group which feels especially vulnerable from certain types of aggression. To tackle women’s personal safety problems several cities around the world introduced transport for women only. Data from Mexico City and its metropolitan area is used to examine how the characteristics of female travellers and of their trips impact upon travel satisfaction with public transport for women only for different travel modes (metro, city bus, and metropolitan BRT) and users based on their previous victimisation. This chapter demonstrates that female travellers’ appreciation of public transport for women only services varies as a function of some of their socio-demographic and travel characteristics (some age groups, trip purposes, and travel frequency). Considering the strength of the marginal effect’s coefficient from a number of ordered logit models three travel attributes should be prioritised: (1) level of satisfaction with travelling with women only, (2) reducing exposure to verbal aggressions, (3) and meeting travellers’ waiting time expectations. This study may help stakeholders identify, target, and prioritise female travellers’ groups which are least satisfied with the service. In addition, this chapter provides some policy recommendations and highlights the role of infrastructure that may help improve the overall travel experience.
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| 4. |
- Hernandez-Alejandro, Roberto, et al.
(författare)
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Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) procedure for colorectal liver metastasis
- 2020
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Ingår i: International Journal of Surgery. - : ELSEVIER. - 1743-9191 .- 1743-9159. ; 82, s. 103-108
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Forskningsöversikt (refereegranskat)abstract
- Since first described, Associating Liver Partition and Portal Vein Ligation for Staged Hepatectomy (ALPPS) has garnered boisterous praise and fervent criticism. Its rapid adoption and employment for a variety of indications resulted in high perioperative morbidity and mortality. However recent risk stratification, refinement of technique to reduce the impact of stage I and progression along the learning curve have resulted in improved outcomes. The first randomized trial comparing ALPPS to two stage hepatectomy (TSH) for colorectal liver metastases (CRLM) was recently published demonstrating comparable perioperative morbidity and mortality with improved resectability and survival following ALPPS. In this review, as ALPPS enters the thirteenth year since conception, the current status of this contentious two stage technique is presented and best practices for deployment in the treatment of CRLM is codified.
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| 5. |
- Khuyagbaatar, J., et al.
(författare)
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Search for elements 119 and 120
- 2020
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Ingår i: Physical Review C. - 2469-9985. ; 102:6
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Tidskriftsartikel (refereegranskat)abstract
- A search for production of the superheavy elements with atomic numbers 119 and 120 was performed in the 50Ti+249Bk and 50Ti+249Cf fusion-evaporation reactions, respectively, at the gas-filled recoil separator TASCA at GSI Darmstadt, Germany. Over four months of irradiation, the 249Bk target partially decayed into 249Cf, which allowed for a simultaneous search for both elements. Neither was detected at cross-section sensitivity levels of 65 and 200 fb for the 50Ti+249Bk and 50Ti+249Cf reactions, respectively, at a midtarget beam energy of Elab = 281.5 MeV. The nonobservation of elements 119 and 120 is discussed within the concept of fusion-evaporation reactions including various theoretical predictions on the fission-barrier heights of superheavy nuclei in the region of the island of stability.
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| 6. |
- Martin-Torres, Javier, et al.
(författare)
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The HABIT (HabitAbility: Brine Irradiation and Temperature) environmental instrument for the ExoMars 2022 Surface Platform
- 2020
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Ingår i: Planetary and Space Science. - : Elsevier. - 0032-0633 .- 1873-5088. ; 190
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Tidskriftsartikel (refereegranskat)abstract
- The HABIT (HabitAbility: Brine Irradiation and Temperature) instrument is a European payload of the ExoMars 2022 Surface Platform Kazachok that will characterize the present-day habitability at its landing place in Oxia Planum, Mars. HABIT consists of two modules: (i) EnvPack (Environmental Package) that monitors the thermal environment (air and ground), the incident ultraviolet radiation, the near surface winds and the atmospheric dust cycle; and (ii) BOTTLE (Brine Observation Transition To Liquid Experiment), an In-situ Resource Utilization instrument to produce liquid water for future Mars exploration. BOTTLE will be used also to investigate the electrical conductivity properties of the martian atmosphere, the present-day atmospheric-ground water cycle and to evaluate if liquid water can exist on Mars in the form of brines, and for how long. These variables measured by HABIT are critical to determine the present and future habitability of the martian surface. In this paper, we describe in detail the HABIT instrument and sensors, together with the calibration of its Flight Model (FM) and the Engineering Qualification Model (EQM) versions. The EnvPack module has heritage from previous missions operating on the surface of Mars, and the environmental observations of its sensors will be directly comparable to those delivered by those missions. HABIT can provide information of the local temperature with ±0.2 °C accuracy, local winds with ±0.3 m/s, surface brightness temperature with ±0.8 °C, incident UV irradiance with 10% error of its absolute value in the UV-A, UV-B, UV-C ranges, as well as in the total UV-ABC range, and two additional wavebands, dedicated to ozone absorption. The UV observations can be used to derive the total opacity column and thus monitor the dust and ozone cycles. BOTTLE can demonstrate the hydration state of a set of four deliquescent salts, which have been found on Mars (calcium chloride, ferric sulphate, magnesium perchlorate and sodium perchlorate) by monitoring their electric conductivity (EC). The EC of the air and the dry salts under Earth ambient, clean room conditions is of the order of 0.1 μScm−1. We have simulated HABIT operations, within an environmental chamber, under martian conditions similar to those expected at Oxia Planum. For dry, CO2 atmospheric conditions at martian pressures, the air EC can be as low as 10−8 μScm−1, however it increases with the relative humidity (RH) percentage. The laboratory experiments show that after an increase from 0 to 60% RH within a few hours, the EC of the air increased up to 10−1 μScm−1, magnesium perchlorate hydrated and reached values of 10 μScm-1, whereas calcium chloride deliquesced forming a liquid state with EC of 102 μScm−1. HABIT will operate with a regular cadence, through day and night. The Electronic Unit (EU) is protected with a heater that is activated when its temperature is below −33 °C and disabled if the temperature of the surface platform rises above −30 °C. Additionally, the heaters of the BOTTLE unit can be activated to dehydrate the salts and reset the experiment. HABIT weighs only 918 g. Its power consumption depends on the operation mode and internal temperature, and it varies between 0.7 W, for nominal operation, and 13.1 W (when heaters are turned on at full intensity). HABIT has a baseline data rate of 1.5 MB/sol. In addition to providing critical environmental observations, this light and robust instrument, will be the first demonstrator of a water capturing system on the surface of Mars, and the first European In-Situ Resource Utilization in the surface of another planet.
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| 7. |
- Mathanlal, Thasshwin, et al.
(författare)
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ATMO-Vent : an adapted breathing atmosphere for COVID-19 patients
- 2020
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Ingår i: HardwareX. - : Elsevier. - 2468-0672. ; 8
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Tidskriftsartikel (refereegranskat)abstract
- The ongoing worldwide pandemic of coronavirus disease 2019 (COVID-19), has been one of the most significant challenges to humankind in centuries. The extremely contagious nature of the SARS-CoV-2 virus has put forth an immense pressure on the health sector. In order to mitigate the stress on the healthcare systems especially to battle the crisis of mechanical ventilators, we have designed a modular, and robust DIY ventilator, ATMO-Vent (Atmospheric Mixture Optimization Ventilator) which can be fully mounted within two days by two operators. The ATMO-Vent has been designed using low-cost, robust, Commercial Off The Shelf (COTS) components, with many features comparable to a full-fledged ventilator. ATMO-Vent has been designed based on the United Kingdom Medicines & Healthcare products Regulatory Agency (UK-MHRA) guidelines for Rapidly Manufactured Ventilator System (RMVS), yet scalable to the specific requirements of different countries. ATMO-Vent is capable of adjusting the Fraction of Inspiratory Oxygen (FiO2) levels, Tidal Volume (TV), frequency of breaths, Inspiratory/Expiratory ratio (I/E), Peak Inspiratory Pressure (PIP) and Positive End-Expiratory Pressure (PEEP). ATMO-Vent can operate in two modes - Continuous Mandatory Ventilation (CMV) using Volume-Controlled Ventilation (VCV) and in Assisted Control (AC) mode with pressure triggered by the patient. ATMO-Vent has undergone rigorous testing and qualifies under Class B Electric and Magnetic Compatibility (EMC) requirements of EN 55011 CISPR 11 standards.
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| 8. |
- Prats-Uribe, Albert, et al.
(författare)
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High-density lipoprotein characteristics and coronary artery disease : a Mendelian randomization study
- 2020
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Ingår i: Metabolism. - : W B SAUNDERS CO-ELSEVIER INC. - 0026-0495 .- 1532-8600. ; 112
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Tidskriftsartikel (refereegranskat)abstract
- Background: To assess whether genetically determined quantitative and qualitative HDL characteristics were independently associated with coronary artery disease (CAD). Methods: We designed a two-sample multivariate Mendelian randomization study with available genome-wide association summary data. We identified genetic variants associated with HDL cholesterol and apolipoprotein A-I levels, HDL size, particle levels, and lipid content to define our genetic instrumental variables in one sample (Kettunen et al. study, n = 24,925) and analyzed their association with CAD risk in a different study (CARDloGRAMplusC4D, n = 184,305). We validated these results by defining our genetic variables in another database (METSINI, n = 8372) and studied their relationship with CAD in the CARDloGRAMplusC4D dataset. To estimate the effect size of the associations of interest adjusted for other lipoprotein traits and minimize potential pleiotropy, we used the Multi-trait-based Conditional & Joint analysis. Results: Genetically determined HDL cholesterol and apolipoprotein A-I levels were not associated with CAD. HDL mean diameter (beta = 027 [95%CI = 0.19; 0.35]), cholesterol levels in very large HDLs (beta = 0.29 (95%CI = 0.17; 0.40]), and triglyceride content in very large HDIs (beta = 0.14 [95%CI = 0.040; 025]) were directly associated with CAD risk, whereas the cholesterol content in medium-sized HDLs (beta = -0.076 [95%CI = -0.10; -0.052]) was inversely related to this risk. These results were validated in the METSIM-CARDloGRAMplu5C4D data. Conclusions: Some qualitative HDL characteristics (related to size, particle distribution, and cholesterol and triglyceride content) are related to CAD risk while HDL cholesterol levels are not. (C) 2020 Elsevier Inc. All rights reserved.
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| 9. |
- Soria-Salinas, Álvaro, et al.
(författare)
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Development of a wind retrieval method for low-speed low-pressure flows for ExoMars
- 2020
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Ingår i: Applied Thermal Engineering. - : Elsevier. - 1359-4311 .- 1873-5606. ; 180
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Tidskriftsartikel (refereegranskat)abstract
- Forced convective heat transfer from three horizontally inclined rectangular-based cylinders (rods) has been studied experimentally under representative Martian near-surface air flows in the Aarhus Wind Tunnel Simulator (AWTS), Denmark. The testing campaign was developed for the HABIT (Habitability: Brines, Irradiation and Temperature) instrument, European payload on board the ExoMars 2022 Kazachok surface platform. The average heat transfer coefficient was determined from steady CO2 flows at a pressure of 9.9 mbar, an ambient temperature of ∼25 °C, and for horizontal free-stream velocities between 0.8 and 12 m/s. A retrieval algorithm to derive the wind speed from the average heat transfer coefficient estimated at each of the three HABIT Air Temperature Sensors (ATS) rods was calibrated within the AWTS. The ATS rods are placed one at the front of the instrument structure (ATS2) and two on the sides (ATS1 and ATS3); and under Martian atmospheric conditions these rods serve as cooling fins. Several relationships between the Nusselt number and the Reynolds and Prandtl numbers reported in the literature were evaluated to model convective heat transfer from the ATS rods. Where needed, corrections to account for radiative heat transfer within the AWTS were implemented. The final retrieval method demonstrated that wind speed can be retrieved for frontal winds in the range of 0–10 m/s, with an error of ±0.3 m/s, using the cooling profile of the ATS rod 3, and for lateral winds in the range of 0–6 m/s, with an error of ±0.3 m/s, using the ATS rod 2 cooling profile.
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| 10. |
- Soria-Salinas, Álvaro, et al.
(författare)
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Wind retrieval from temperature measurements from the Rover Environmental Monitoring Station/Mars Science Laboratory
- 2020
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Ingår i: Icarus. - : Elsevier. - 0019-1035 .- 1090-2643. ; 346
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Tidskriftsartikel (refereegranskat)abstract
- This work presents a novel method for the real-time retrieval of wind speed on the surface of Mars that uses temperature measurements from the Rover Environmental Monitoring Station (REMS) instrument onboard the Curiosity rover of the Mars Science Laboratory (MSL) mission. After final failure of the Wind Sensor (WS) in sol 1491, REMS has not been providing wind data. The new wind retrieval approach that we propose may eventually be able to supply MSL with wind values for contextualizing the roverâôs operations and for meteorological studies on the surface of Mars. The new method is based on forced convection modeling of the Air Temperature Sensors (ATS) of REMS as thin rods immersed in the extreme low-pressure and high-radiating atmospheric conditions of the Martian thermal boundary layer at a height of ∼" role="presentation" style="box-sizing: border-box; margin: 0px; padding: 0px; display: inline-block; line-height: normal; font-size: 16.2px; word-spacing: normal; overflow-wrap: normal; white-space: nowrap; float: none; direction: ltr; max-width: none; max-height: none; min-width: 0px; min-height: 0px; border: 0px; position: relative;">∼ 1.5 m from the surface. A preliminary validation of the possibilities and limitations of this retrieval has been performed using comparative analysis with existing REMS wind field-site data for the same sols that are available at the Planetary Data System (PDS). We have developed both a ”coarse” approach, in which wind speed is determined with no regard to wind direction, and a ”refined” method, in which it is attempted to determine both wind speed and direction. Assuming the previously reported WS retrieval errors of 20% for the wind speed, we report an agreement to the WS values of wind speed ranging from 36.4% to 77% of the acquisition time for the ”coarse” approach, depending on the sol examined. These promising results are limited to only evening extended acquisitions from 18:00 to 21:00 local mean solar time (LMST). This method could be applied to daytime conditions. The results suggest a new optimal orientation for wind speed retrieval of 60°clockwise with respect to the forward direction of the Curiosity rover, although the technique is not yet ready to be considered for planning of the Curiosity rover operations. This method could extend the wind characterization of the Gale Crater for future Curiosity rover data acquisitions by recycling air temperature measurements and provide the scientific community with a data set for future comparative analysis with the Temperature and Wind Sensors for InSight (TWINS)/InSight, the HabitAbility: Brines, Irradiation and Temperature (HABIT)/ExoMars 2020, and the Mars Environmental Dynamics Analyzer (MEDA)/Mars 2020 rover instruments.
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