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Träfflista för sökning "WFRF:(Wang Xiao Ru) srt2:(2003-2004)"

Sökning: WFRF:(Wang Xiao Ru) > (2003-2004)

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1.
  • Zeng, Qing-Yin, et al. (författare)
  • Detection and quantification of Wallemia sebi in aerosols by real-time PCR, conventional PCR, and cultivation
  • 2004
  • Ingår i: Applied and Environmental Microbiology. - : American Society for Microbiology. - 0099-2240 .- 1098-5336. ; 70:12, s. 7295-7302
  • Tidskriftsartikel (refereegranskat)abstract
    • Wallemia sebi is a deuteromycete fungus commonly found in agricultural environments in many parts of the world and is suspected to be a causative agent of farmer's lung disease. The fungus grows slowly on commonly used culture media and is often obscured by the fast-growing fungi. Thus, its occurrence in different environments has often been underestimated. In this study, we developed two sets of PCR primers specific to W. sebi that can be applied in either conventional PCR or real-time PCR for rapid detection and quantification of the fungus in environmental samples. Both PCR systems proved to be highly specific and sensitive for W. sebi detection even in a high background of other fungal DNAs. These methods were employed to investigate the presence of W. sebi in the aerosols of a farm. The results revealed a high concentration of W. sebi spores, 10(7) m(-3) by real-time PCR and 10(6) m(-3) by cultivation, which indicates the prevalence of W. sebi in farms handling hay and grain and in cow barns. The methods developed in this study could serve as rapid, specific, and sensitive means of detecting W. sebi in aerosol and surface samples and could thus facilitate investigations of its distribution, ecology, clinical diagnosis, and exposure risk assessment.
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2.
  • Zeng, Qing-Yin, et al. (författare)
  • Development of mitochondrial SSU rDNA-based oligonucleotide probes for specific detection of common airborne fungi
  • 2003
  • Ingår i: Molecular and Cellular Probes. - 0890-8508 .- 1096-1194. ; 17:6, s. 281-288
  • Tidskriftsartikel (refereegranskat)abstract
    • In this study we sequenced partial mitochondrial small subunit rDNA from 32 fungal strains representing 31 species from 16 genera. Most of these species are common airborne fungi and pathogens that may cause various public health concerns. Sequence alignment showed several conserved and highly variable regions. The variable regions were deployed to design oligonucleotide probes for each fungal species. The specificity of the designed probes was first examined through homology search against GenBank database then further verified through hybridization experiments to 38 fungal strains. A total of 23 probes were verified as specific to 15 fungal species commonly detected in living and working environments. These new probes will have potential applications in clinical diagnosis and public health-related environmental monitoring.
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3.
  • Zeng, Qing-Yin, et al. (författare)
  • Extensive set of mitochondrial LSU rDNA-based oligonucleotide probes for the detection of common airborne fungi.
  • 2004
  • Ingår i: FEMS Microbiology Letters. - : Oxford University Press (OUP). - 0378-1097 .- 1574-6968. ; 237:1, s. 79-87
  • Tidskriftsartikel (refereegranskat)abstract
    • Fungi exist in every indoor and outdoor environment. Many fungi are toxigenic or pathogens that may cause various public health concerns. Rapid and accurate detection and identification of fungi require specific markers. In this study, partial mitochondrial large subunit rDNA was amplified and sequenced from 32 fungal strains representing 31 species from 14 genera. Based on the sequence variation pattern, 26 oligonucleotide probes were designed for their discrimination. The specificity of the probes was evaluated through homology search against GenBank database and hybridization examination on 38 fungal strains. The 26 probes were verified as highly specific to 20 fungal species. A two-step detection procedure through PCR followed by probe hybridization gave ten-fold increase in detection sensitivity than single-step PCR assay and would be a practical approach for environmental sample screening. The probes developed in this study can be applied in clinical diagnosis and environmental monitoring of fungal agents.
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  • Resultat 1-3 av 3
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refereegranskat (3)
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Wang, Xiao-Ru (3)
Zeng, Qing-Yin (3)
Rasmuson-Lestander, ... (2)
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