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- Jarkrans, Torsten, et al.
(författare)
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Grading of transitional cell bladder carcinoma by image analysis of histological sections
- 1995
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Ingår i: Analytical Cellular Pathology. - : ELSEVIER SCI PUBL IRELAND LTD. - 0921-8912 .- 1878-3651. ; 8:2, s. 135-158
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Tidskriftsartikel (refereegranskat)abstract
- Image analysis of histological sections was used to achieve a more objective malignancy grading of transitional cell carcinoma of the bladder. Images from Feulgen-stained sections from a clinical material of 197 tumours were analyzed. Features at various
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- Ranefall, Petter, et al.
(författare)
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Automatic quantification of immunohistochemically stained cell nuclei based on standard reference cells
- 1998
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Ingår i: Analytical Cellular Pathology. - 0921-8912 .- 1878-3651. ; 17:2, s. 111-23
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Tidskriftsartikel (refereegranskat)abstract
- A fully automatic method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions, is presented. Agarose embedded cultured fibroblasts were fixed, paraffin embedded and sectioned at 4 microm. They were then stained together with 4 microm sections of the test specimen obtained from bladder cancer material. A colour based classifier is automatically computed from the control cells. The method was tested on formalin fixed paraffin embedded tissue section material, stained with monoclonal antibodies against the Ki67 antigen and cyclin A protein. Ki67 staining results in a detailed nuclear texture with pronounced nucleoli and cyclin A staining is obtained in a more homogeneously distributed pattern. However, different staining patterns did not seem to influence labelling index quantification, and the sensitivity to variations in light conditions and choice of areas within the control population was low. Thus, the technique represents a robust and reproducible quantification method. In tests measuring proportions of stained area an average standard deviation of about 1.5% for the same field was achieved when classified with classifiers created from different control samples.
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- Ranefall, Petter, et al.
(författare)
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Automatic quantification of microvessels using unsupervised image analysis
- 1998
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Ingår i: Analytical Cellular Pathology. - : IOS PRESS. - 0921-8912 .- 1878-3651. ; 17:2, s. 83-92
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Tidskriftsartikel (refereegranskat)abstract
- An automatic method for quantification of images of microvessels by computing area proportions and number of objects is presented. The objects are segmented from the background using dynamic thresholding of the average component size histogram. To be able
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- Wester, Kenneth, et al.
(författare)
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Automatic quantification of microvessel density in urinary bladder carcinoma
- 1999
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Ingår i: British Journal of Cancer. - : CHURCHILL LIVINGSTONE. - 0007-0920 .- 1532-1827. ; 81:8, s. 1363-1370
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Tidskriftsartikel (refereegranskat)abstract
- Seventy-three TUR-T biopsies from bladder carcinoma were evaluated regarding microvessel density, defined as microvessel number (nMVD) and cross-section endothelial cell area (aMVD). A semi-automatic and a newly developed, automatic image analysis techniq
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| 10. |
- Wester, Kenneth
(författare)
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Quantitative immunochemistry in tissue sections : With special reference to urinary bladder carcinoma
- 1999
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Doktorsavhandling (övrigt vetenskapligt/konstnärligt)abstract
- Urinary bladder carcinoma affects about 2 000 people in Sweden every year. It is a heterogeneous disease and for adequate treatment decisions, prognostic tools are needed. Many of these are based on immunohistochemistry (MC) and hampered by poorly reproducible methodologies and subjective evaluations.This investigation aimed to evaluate and define the conditions for an objective and reproducible quantification of(MC) applied to routinely processed (formalin-fixed and paraffin-embedded) tissue material. To establish theseconditions, proliferation-related proteins, tumour-suppressor proteins and endothelial cell markers were studiedThree computerised colour-based image analysis methods, aiming to reduce user interaction, were developed and evaluated for variability and reproducibilty in quantification of IHC applied to histological sections. Themethods proved stable to variations in focus- and light-settings but revealed a sensitivity to poor IHC-qualityand choice of chromogen. Implementation of an external control system, used as base for computing theclassifier, further increased the stability.Using these methods, effects of varying tissue-handling and immunostaining conditions were analysed. Storageof both paraffin-blocks and -sections was deleterious to immunoreactivity but could be compensated for bytayloring of the heat induced epitope retrieval (HIER) protocol.A control system for MC based on cultured cells was developed to reflect variations in tissue-handling and IHC-conditions. Control and biopsy material showed similar sensitivity to section storage, MC- and HIER-protocolvariations. Furthermore, controls reflected variations in fixation time and proved stable to variations in sectionthickness.
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