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  • Håkansson, Kristina, et al. (författare)
  • A biosensor for the analysis of acetonitrile
  • 2004
  • Ingår i: Biosensors & Bioelectronics. - Elsevier. - 1873-4235. ; 19:7, s. 721-726
  • Tidskriftsartikel (refereegranskat)abstract
    • A biosensor for monitoring acetonitrile was constructed. A mixed culture was taken from a degradation reactor and mounted on top of a Clark electrode. The amperometric biosensor was placed in a flow-through cell and integrated into a flow injection system. The metabolic response in terms of oxygen consumption was well correlated to the concentration of acetonitrile in standard solutions. However, when the reaction products, acetic acid and ammonia, were also present, the response was erratic, due to the additional metabolic reaction on acetate. By introducing a hydrophobic barrier it was possible to eliminate the negative influence of these charged products and thus to improve the operational selectivity of the sensor. The biosensor showed good stability for analysis during at least 6 days and future work will focus on using it for monitoring and control of degradation processes. (C) 2003 Elsevier B.V. All rights reserved.
  • Håkansson, Kristina, et al. (författare)
  • Microbial degradation of acetonitrile using a suspended-carrier biofilm process
  • 2002
  • Ingår i: Biotechnology Letters. - Springer. - 1573-6776. ; 24:4, s. 287-291
  • Tidskriftsartikel (refereegranskat)abstract
    • A microbial process for the destruction of acetonitrile has been developed. Suitable microorganisms were enriched from activated sludge by cultivation with a high selection pressure when acetonitrile was the sole source of carbon and nitrogen. The process is based on cultivating the microorganisms as a biofilm on plastic carriers. These are utilised in a stirred-tank reactor with continuous feeding of 40-150 mM acetonitrile and an outlet for spent medium. The observed efficiency in the conversion process was from 53-100%, depending on the organic load. To improve the capacity and reliability of the process, a two-step process was developed with two stirred-tank reactors coupled in series. With such an arrangement, conversion efficiencies from 92-100% were achieved when the organic load was 2 g acetonitrile l(-1) d(-1) in the first reactor.
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