| 1. |
- Ahlqvist, Josefin, et al.
(author)
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Affinity binding of inclusion bodies on supermacroporous monolithic cryogels using labeling with specific antibodies
- 2006
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In: Journal of Biotechnology. - : Elsevier BV. - 0168-1656 .- 1873-4863. ; 122:2, s. 216-225
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Journal article (peer-reviewed)abstract
- A new chromatographic method based on affinity supermacroporous monolithic cryogels is developed for binding and analyzing inclusion bodies during fermentation. The work demonstrated that it is possible to bind specific IgG and IgY antibodies to the 15 and 17 amino acids at the terminus ends of a 33 kDa target protein aggregated as inclusion bodies. The antibody treated inclusion bodies from lysed fermentation broth can be specifically retained in protein A and pseudo-biospecific ligand sulfamethazine modified supermacroporous cryogels. The degree of binding of IgG and IgY treated inclusion bodies to the Protein A and sulfamethazine gels are investigated, as well as the influence of pH on the sulfamethazine ligand. Optimum binding of 78 and 72% was observed on both protein A and sulfamethazine modified cryogel columns, respectively, using IgG labeling of the inclusion bodies. The antibody treated inclusion bodies pass through unretained in the sulfamethazine supermacroporous gel at pH that does not favour the binding between the ligand on the gel and the antibodies on the surface of inclusion bodies. Also the unlabeled inclusion bodies went through the gel unretained, showing no non-specific binding or trapping within the gel. These findings may very well be the foundation for the building of a powerful analytical tool during fermentation of inclusion bodies as well as a convenient way to purify them from fermentation broth. These results also support our earlier findings [Kumar, A., Plieva, F.M., Galaev, I.Yu., Mattiasson, B.. 2003. Affinity fractionation of lymphocytes using a monolithic cyogel. J. Immunol. Methods 283, 185-194] with mammalian cells that were surface labeled with specific antibodies and recognized on protein A supermacroporous gels. A general binding and separation system can be established on antibody binding cryogel affinity matrices.
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| 2. |
- Hallström, Lena, 1958-, et al.
(author)
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Familiar factors and breakfast consumption among European adolescents: The HELENA-study
- 2009
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In: International Congress of Nutrition : 19th Congress, Bangkok, October 2009. - Bangkok : Kager Medical and Scientific Publishers. - 9783805592055 - 3805592051 ; , s. 627-627
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Conference paper (peer-reviewed)abstract
- Objectives: To identify familiar factors influencing breakfast consumption and choices at breakfast in European adolescents participating in the Healthy Lifestyle in Europe by Nutrition in Adolescence (HELENA) study.Methods: The HELENA is a multicentre school-based study. A total of 3546 adolescents aged12.5-17.49 years responded to questions about breakfast habits and determinants about eating behavior during the academic year 2006-2007.Results: 66% of boys and 58% of girls indicated to regularly consume breakfast. Boys whose parents support them to eat healthy are more likely to consume breakfast (OR, 0.61; 95% CI, 0.43-0.85). Girls whose peers eat healthy are more likely to consume breakfast (OR, 0.68; 95% CI, 0.51-0.91). Hunger (59%), taste (57%), health (48%) and parents (45%) are the most important factors influencing adolescents’ food choices at breakfast.Conclusion: Parents’ and peers’ influences should be considered in the development and testing of intervention strategies.
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| 3. |
- Hallström, Lena, 1958-, et al.
(author)
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Validation of a computerized 24h-recall in European Adolescents from the HELENA study
- 2009
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Conference paper (peer-reviewed)abstract
- Introduction: The HELENA study (Healthy Lifestyle in Europe by Nutrition in Adolescents) has been performed in ten cities around Europe. The study has been using a computerized 24h-recall program called “Young adolescents' nutrition assessment on computer” (YANA-C). The program has been developed, used and validated in Belgium. In the HELENA study all countries translated and culturally adapted the YANA-C software. The validity of the results might depend on the translation and compliance to the test protocol of the YANA-C. Therefore it is important to validate YANA-C in other countries.Aim: To validate the computerized 24h-recall, YANA-C, against estimated dietary records, in adolescents (13 and 16 years of age) in three additional countries (Sweden, Hungary and Spain) belonging to the HELENA study, and to compare it with the outcome from the Belgium validation study.Method: More than three hundred adolescents completed YANA-C on two non-consecutive days. One to five days later, the adolescents completed an estimated dietary record on two non-consecutive comparable days as those for which YANA-C was completed. Wilcoxon signed rank test is used to compare the intakes of food groups and nutrients (energy, carbohydrates, protein, fat, fiber, calcium, vitamin C, iron) between both methods.Results: The results from Belgium showed a significant lower intake in energy (p<0.001), carbohydrate (p<0.01), protein (p<0.05), fat (p<0.01), fiber (p<0.001), vitamin C (p<0.05) and iron (p<0.05) in the dietary record compared to YANA-C. The Belgium results had a higher energy and nutrient intake (except calcium) from YANA-C comparing to the dietary record. Preliminary results from the Hungarian sample (n=99) show a significant lower intake of carbohydrates (p<0.001), protein (p<0.001) and a higher intake of fat (<0.05), fiber (<0.05), calcium (<0.01) in the dietary records compared to the YANA-C. There was no significant difference in energy, vitamin C and iron.Conclusion: The Belgium and Hungarian samples showed different results of the validation studies. This shows the importance to validate the results from countries using YANA-C database. Analyses on a food group level might help to explain these differences.HELENA study takes place with the financial support of the European Community (FP6-2003-Food-2-A, FOOD-2003-T2.4, Contract FOOD-CT-2005-007034)
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| 4. |
- Messing, Ingmar, et al.
(author)
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Bygg inte på kultur- och åkermark
- 2009
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In: Upsala nya tidning. - Uppsala. - 1104-0173. ; :2 maj, s. 4-
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Journal article (pop. science, debate, etc.)
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| 5. |
- Odlare, Monica, et al.
(author)
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Effect of wood ash and compost on nitrification and denitrification in soil.
- 2009
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In: Applied Energy. - : Elsevier BV. - 0306-2619 .- 1872-9118. ; 86:1, s. 74-80
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Journal article (peer-reviewed)abstract
- Wood ash from biofuel combustion plants and compost from source-separated household waste are commonly spread on forest, agricultural and horticultural soils as a valuable Source of plant nutrients. However, due to anthropogenic activities, wood ash may contain high concentrations of heavy metals. Heavy metals are toxic to microorganisms and therefore, soil microbial response to wood ash Should be considered when soil is amended with ash. Compost is known to improve soil structure and may also act as a bioremediating agent, mitigating any toxic effects of wood ash on soil microorganisms. In the present study, the aim was to investigate whether wood ash has any toxic effect on soil microbial activity and, if this is the case, whether Compost Could mitigate these effects. The effect of wood fly ash oil potential ammonium oxidation rate (PAO) and potential denitrification rate (PDA) in arable soil was investigated in one dose-response assay and in two pot experiments With or Without plants, respectively. The treatments were amendment with wood fly ash, compost or a combination of wood fly ash and compost. PAO and PDA were assessed immediately or after 7 and 90 days in the different experiments. Wood fly ash decreased PDA to 16-56% compared to the control, while PAO varied between 82% and 205%. Sole compost addition stimulated both processes. This positive effect was also observed in the combined wood fly ash-compost treatment. In conclusion, wood ash had a toxic effect on PDA, both on an immediate, short-term and long-term basis. Amendment of compost clearly mitigated this toxic effect. The observed toxicity could be an effect of the metal content of ash.
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