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Sökning: hsv:(LANTBRUKSVETENSKAPER) hsv:(Veterinärmedicin) > Rodriguez Martinez Heriberto

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1.
  • Gizejewski, Z, et al. (författare)
  • Genital and sperm characteristics of wild, free rangingred deer stags (Cervus elaphus L) hunted in different regions of Poland
  • 2010
  • Ingår i: Wildlife Biology in Practice. - Braga, Portugal : Sociedade Portuguesa de Vida Selvagem,Portuguese Wildlife Society. - 1646-1509 .- 1646-2742. ; 6, s. 81-91
  • Tidskriftsartikel (refereegranskat)abstract
    • In an attempt to establish reference values for sperm morphology in wild red deer, genital tracts were collected from thirty-six 3-11 years old free-ranging, wild red deer stags (Cervus elaphus L) shot down during 3 consecutive mating seasons (1996-1998) at three different environmental regions of Poland, defining two major ecotypes: (i) highland (outer eastern Carpathian range, Bieszczady mountains) and, (ii) lowland (Mazuria and Pomerania) and studied within 4.5h-49h after death for testis (T), epididymides (E) and vesicular gland (VG) variables. Spermatozoa collected from the E-cauda were examined for motility and morphology (light and electron microscopy levels). Both T size and weight and VS-weight differed with age (P<0.05-0.01) while habitat influenced T size and weight (P<0.01) a well as sperm motility (P<0.05). Neither sperm numbers nor morphology showed significant differences, mostly owing tothe large variation recorded among stags (range 1-72%). Domain-grouped sperm morphological deviations were <5%, the mean total proportion of abnormal spermatozoa ranging 7.2-17.5%. Although variation was present, the values ought to be used as reference for spermiogrammes.
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2.
  • Macias Garcia, B., et al. (författare)
  • Single-Layer Centrifugation Through Colloid Positively Modifies the Sperm Subpopulation Structure of Frozen-Thawed Stallion Spermatozoa
  • 2009
  • Ingår i: Reproduction in domestic animals. - : Wiley-Blackwell. - 0936-6768 .- 1439-0531. ; 44:3, s. 523-526
  • Tidskriftsartikel (refereegranskat)abstract
    • The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E (TM)). After freezing and thawing, four sperm subpopulations were identified, listed as FT1 to FT4. While subpopulations FT1 and FT2 were characterized by low sperm velocity, high velocities characterized the ones called FT3 and FT4. The single-layer centrifugation (SLC)-handled sperm sample was enriched in subpopulation FT3, reaching a proportion of 82.6% of the present spermatozoa, in contrast with the non-filtered control post-thawed semen, where this sperm subpopulation only accounted for 16.3% of the total. It is concluded that in the equine industry, the SLC is a practical, easy-to-perform approach to improve the quality of equine frozen-thawed semen samples.
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3.
  • Balao da Silva, C. M., et al. (författare)
  • Effect of Overnight Staining on the Quality of Flow Cytometric Sorted Stallion Sperm: Comparison with Tradtitional Protocols
  • 2014
  • Ingår i: Reproduction in domestic animals. - : Wiley. - 0936-6768 .- 1439-0531. ; 49:6, s. 1021-1027
  • Tidskriftsartikel (refereegranskat)abstract
    • Flow cytometry is considered the only reliable method for the separation of X and Y chromosome bearing spermatozoa in equines. The MoFlo SX DP sorter is highly efficient, allowing the production of foals of the desired sex. However, to achieve acceptable pregnancy rates the currently used protocol requires working with fresh semen obtained close to, or at, the sorting facility. An alternative protocol was tested during two consecutive breeding seasons. Fresh stallion semen was cooled for 20 h, during which staining with Hoechst 33342 took place. On the following day, this sample was flow sorted and compared with spermatozoa from the same ejaculate that had been sexed on the previous day. All sperm parameters evaluated remained unchanged when fresh sorted and refrigerated sorted semen were compared. Pre-sorting storage at 5 degrees C did not alter sperm velocities nor kinetics, viability or membrane permeability, production of reactive oxygen species, mitochondrial membrane potential or DNA fragmentation index of the sorted sample. The findings open for the possibility of using semen from stallions housed far from the sorting facilities. Processed and stained sperm could be shipped refrigerated on the previous day, sorted and inseminated on the next day.
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4.
  • Balao da Silva, C. M., et al. (författare)
  • Flow Cytometric Chromosomal Sex Sorting of Stallion Spermatozoa Induces Oxidative Stress on Mitochondria and Genomic DNA
  • 2016
  • Ingår i: Reproduction in domestic animals. - : WILEY-BLACKWELL. - 0936-6768 .- 1439-0531. ; 51:1, s. 18-25
  • Tidskriftsartikel (refereegranskat)abstract
    • To date, the only repeatable method to select spermatozoa for chromosomal sex is the Beltsville sorting technology using flow cytometry. Improvement of this technology in the equine species requires increasing awareness of the modifications that the sorting procedure induces on sperm intactness. Oxidative stress is regarded as the major damaging phenomenon, and increasing evidence regards handling of spermatozoa - including sex sorting - as basic ground for oxidative damage. The aim of this study was to disclose whether the flow cytometric sorting procedure increases the production of reactive oxygen species (ROS), and to identify if ROS production relates to DNA damage in sorted spermatozoa using specific flow cytometry-based assays. After sorting, oxidative stress increased from 26% to 33% in pre-and post-incubation controls, to 46% after sex sorting (p < 0.05). Proportions of DNA fragmentation index post-sorting were approximately 10% higher (31.3%); an effect apparently conduced via oxidative DNA damage as revealed by the oxyDNA assay. The probable origin of this increased oxidative stress owes the removal of enough seminal plasma due to the unphysiological sperm extension, alongside a deleterious effect of high pressure on mitochondria during the sorting procedure.
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5.
  • Nagy, S, et al. (författare)
  • Sperm chromatin structure and sperm morphology: Their association with fertility in AI-dairy Ayrshire sires
  • 2013
  • Ingår i: Theriogenology. - : Elsevier. - 0093-691X .- 1879-3231. ; 79:8, s. 1153-1161
  • Tidskriftsartikel (refereegranskat)abstract
    • Prognostic relations between sperm variables and sire fertility are yet elusive. A retrospective analysis of sperm morphology and chromatin stability (studied using sperm chromatin structure assay [SCSA]) and their relation to fertility after AI (as proportions of 60 days of nonreturn to estrous [NRR], corrected NRR, or calving rate) was studied with preselected frozen semen doses from a group (N = 43) of AI-sires of the Finnish Ayrshire breed composed of 50% subfertile bulls (andlt;55% NRR) and 50% fertile sires (andgt;55% NRR). Fertility, indicated by all three parameters, correlated significantly only with the percentage of morphologically normal spermatozoa, a variable which negatively correlated with the percentage of DNA fragmentation at the time of SCSA, thus confirming the value of always having high numbers of morphologically normal spermatozoa in AI-doses. Proportions of major sperm defects also related to fertility but only when considering corrected NRR, not with calving rate, indicating that proportions of normal spermatozoa, a value surpassing differences between sperm laboratory screening methods, might be valuable and could be easily made routine by the industry. Though SCSA as a method is being contested for DNA- and chromatin analyses in the light of epigenetic changes, a particular parameter, the High Green fluorescence, showed the highest values for sperm doses collected from bulls having meiotic problems and containing a high proportion of diploid spermatozoa (approximately 20%) and also in bulls having a reciprocal chromosomal translocation, thus suggesting such a parameter might be useable to discriminate which bulls ought to be studied in more detail, including cytogenetic analyses.
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6.
  • Roca, J., et al. (författare)
  • Non-viable sperm in the ejaculate: Lethal escorts for Contemporary viable sperm
  • 2016
  • Ingår i: Animal Reproduction Science. - : ELSEVIER SCIENCE BV. - 0378-4320 .- 1873-2232. ; 169, s. 24-31
  • Tidskriftsartikel (refereegranskat)abstract
    • Non-viable sperm ("dead sperm") are present invariable numbers in mammalian ejaculates and their number increase substantially when semen is stored, particularly cryopreserved. This review comparatively highlights, with experimental data in porcine, the role-played by non-viable sperm in the outcome of semen used in assisted reproductive technologies. As well, the review discusses our current understanding of their origin and the pathways involved when their large numbers negative influence the functional lifespan of contemporary viable sperm to eventually cause irreversible dysfunction that reduces their fertility potential and their ability to develop healthy embryos. Finally, it highlights procedures currently available to mitigate these harmful effects. (C) 2016 Elsevier B.V. All rights reserved.
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7.
  • Awasthi, H., et al. (författare)
  • Do Cytoplasmic Lipid Droplets Accumulate in Immature Oocytes from Over-Conditioned Repeat Breeder Dairy Heifers?
  • 2010
  • Ingår i: Reproduction in domestic animals. - : Wiley-Blackwell. - 0936-6768 .- 1439-0531. ; 45:5, s. E194-E198
  • Tidskriftsartikel (refereegranskat)abstract
    • One of the main sources of repeat breeding in dairy cattle, caused by fertilization failure or early embryonic death, is metabolic stress during lactation. Nutrition seems also to play a role when the condition is seen in heifers, where oocyte cytoplasmic maturation is impaired. To determine whether over conditioning affects oocyte morphology, immature oocytes were collected by ovum pick-up (OPU) twice weekly during 5 weeks from three over-conditioned repeat breeder dairy heifers (RBH) and two normal virgin heifers (VH, controls) of the Swedish Red breed, monitored by body weight and condition. Oocyte quality was assessed under stereomicroscope and further examined by transmission electron microscope for accumulation of cytoplasmic lipid deposits. After OPU, the RBH yielded more low quality oocytes (60% vs 52% for VH, p = 0.14). The relative occupancy of osmophilic lipid droplets in the cytoplasm was higher in oocytes of bad quality compared with good ones, especially in RBH (p = 0.08) but also in VH (p = 0.11). Moreover, the oocytes from over-conditioned RBH showed higher amounts of cytoplasmic lipid deposits both in good (p = 0.14) and, even more prominent, in bad quality oocytes (p = 0.06). Such accumulation of lipid droplets may imply increased sensitivity to oxidative stress, hinder cytoplasmic maturation and lead to subfertility, as accounted in over-conditioned repeat breeders of the Swedish Red breed.
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8.
  • Balao da Silva, C, et al. (författare)
  • Effect of Hoechst 33342 on stallion spermatozoa incubated in KMT or Tyrodes modified INRA96
  • 2012
  • Ingår i: Animal Reproduction Science. - : Elsevier Masson. - 0378-4320 .- 1873-2232. ; 131:3-4, s. 165-171
  • Tidskriftsartikel (refereegranskat)abstract
    • The only known means of effectively separating populations of X and Y bearing sperms is the Beltsville sexing technology. The technology implies that each individual sperm is interrogated for DNA content, measuring the intensity of the fluorescence after staining the spermatozoa with Hoechst 33342. Because there are no data regarding the effect of the staining on stallion sperm, ejaculates were incubated up to 90 min in presence of 0, 4.5, 9, 22.5, 31.5, 45, 54, 67.5, 76.5 and 90 mu M of Hoechst 33342, in two media, KMT or INRA-Tyrodes. After 40 and 90 min of incubation, motility (CASA) and membrane integrity (flow cytometry after YoPro-1/Eth staining) were evaluated. In KMT extender sperm motility significantly decreased after 45 min of incubation when sperm were incubated in the presence of concentrations of Hoechst of 45 mu M or greater (P andlt; 0.05). When incubated in modified INRA96, stallion spermatozoa tolerated greater concentrations of Hoechst, because sperm motility only decreased when incubated in presence of 90 mu M (P andlt; 0.05) and membrane integrity was not affected. After 90 min of incubation the same effect was observed, but in this case at concentrations over 45 mu M the percentage of total motile sperm was also reduced although only in samples incubated in KMT. To produce this effect in samples incubated in Tyrodes modified INRA 96, Hoechst had to be present at concentrations over 67.5 mu M. Apparently, the detrimental effect of Hoechst to stallion spermatozoa varies depending on the media, and INRA modified extender may be an alternative to KMT.
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9.
  • Barranco, Isabel, et al. (författare)
  • Seminal Plasma Modulates miRNA Expression by Sow Genital Tract Lining Explants
  • 2020
  • Ingår i: Biomolecules. - : MDPI. - 2218-273X. ; 10:6
  • Tidskriftsartikel (refereegranskat)abstract
    • The seminal plasma (SP) modulates the female reproductive immune environment after mating, and microRNAs (miRNAs) could participate in the process. Considering that the boar ejaculate is built by fractions differing in SP-composition, this study evaluated whether exposure of mucosal explants of the sow internal genital tract (uterus, utero-tubal junction and isthmus) to different SP-fractions changed the profile of explant-secreted miRNAs. Mucosal explants retrieved from oestrus sows (n = 3) were in vitro exposed to: Medium 199 (M199, Control) or M199 supplemented (1:40 v/v) with SP from the sperm-rich fraction (SRF), the post-SRF or the entire recomposed ejaculate, for 16 h. After, the explants were cultured in M199 for 24 h to finally collect the media for miRNA analyses using GeneChip miRNA 4.0 Array (Affymetrix). Fifteen differentially expressed (False Discovery Rate (FDR) < 0.05 and Fold-change ≥ 2) miRNAs (11 down- versus 4 up-regulated) were identified (the most in the media of uterine explants incubated with SP from post-SRF). Bioinformatics analysis identified that predicted target genes of dysregulated miRNAs, mainly miR-34b, miR-205, miR-4776-3p and miR-574-5p, were involved in functions and pathways related to immune response. In conclusion, SP is able to elicit changes in the miRNAs profile secreted by female genital tract, ultimately depending SP-composition.
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10.
  • Bergqvist, Ann-Sofi, et al. (författare)
  • Single Layer Centrifugation of Stallion Spermatozoa through Androcoll (TM)-E does not Adversely Affect their Capacitation-Like Status, as Measured by CTC Staining
  • 2011
  • Ingår i: Reproduction in domestic animals. - : Blackwell Publishing. - 0936-6768 .- 1439-0531. ; 46:1, s. e74-e78
  • Tidskriftsartikel (refereegranskat)abstract
    • Contents This study was designed to evaluate the effect of single layer centrifugation (SLC) and subsequent cold storage on stallion sperm capacitation-like status and acrosome reaction. Three stallions were included in the study, with three ejaculates per stallion. The samples were examined 4, 24 and 72 h after collection, extension and SLC, with storage at 6 degrees C. Sperm capacitation-like status was investigated using the fluorescent dye chlortetracycline (CTC). There was no difference in capacitation-like status between colloid-selected and non-selected spermatozoa. Sperm motility decreased significantly during cold storage, whereas the proportion of apparently capacitated spermatozoa increased. There was no change in the proportion of acrosome-reacted spermatozoa. In conclusion, SLC through Androcoll (TM)-E does not adversely affect the capacitation-like status of stallion spermatozoa, although it did increase with time during cold storage.
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