| 1. |
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| 2. |
- Fries, Ingemar, et al.
(författare)
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Verksamhet med bin vid SLU 2011.
- 2012
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Ingår i: Bitidningen. - 0006-3886. ; 111, s. 12-16
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 3. |
- Locke Grandér, Barbara, et al.
(författare)
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SLU redovisar tre studier om effekter på virusnivåer : Effekt på virusnivåer efter varroabekämpning
- 2016
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Ingår i: Bitidningen. - 0006-3886. ; 115, s. 20-23
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Tidskriftsartikel (populärvet., debatt m.m.)abstract
- Varroakvalstret (Varroa destructor) är ett av de största hoten mot biodling världen över. Utan behandling dör angripna bisamhällen vanligtvis inom några år, men inte på grund av själva kvalsterangreppet i sig utan av de virusinfektioner som sprids med och utvecklas effektivare på grund av kvalstren. Många olika slags virus har hittats hos honungsbin, och några av dem är starkt knutna till varroakvalstret.
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| 4. |
- Locke Grandér, Barbara, et al.
(författare)
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Virus Prevalence in Egg Samples Collected from Naturally Selected and Traditionally Managed Honey Bee Colonies across Europe
- 2022
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Ingår i: Viruses. - : MDPI AG. - 1999-4915. ; 14
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Tidskriftsartikel (refereegranskat)abstract
- Monitoring virus infections can be an important selection tool in honey bee breeding. A recent study pointed towards an association between the virus-free status of eggs and an increased virus resistance to deformed wing virus (DWV) at the colony level. In this study, eggs from both naturally surviving and traditionally managed colonies from across Europe were screened for the prevalence of different viruses. Screenings were performed using the phenotyping protocol of the 'suppressed in ovo virus infection' trait but with qPCR instead of end-point PCR and a primer set that covers all DWV genotypes. Of the 213 screened samples, 109 were infected with DWV, 54 were infected with black queen cell virus (BQCV), 3 were infected with the sacbrood virus, and 2 were infected with the acute bee paralyses virus. It was demonstrated that incidences of the vertical transmission of DWV were more frequent in naturally surviving than in traditionally managed colonies, although the virus loads in the eggs remained the same. When comparing virus infections with queen age, older queens showed significantly lower infection loads of DWV in both traditionally managed and naturally surviving colonies, as well as reduced DWV infection frequencies in traditionally managed colonies. We determined that the detection frequencies of DWV and BQCV in honey bee eggs were lower in samples obtained in the spring than in those collected in the summer, indicating that vertical transmission may be lower in spring. Together, these patterns in vertical transmission show that honey bee queens have the potential to reduce the degree of vertical transmission over time.
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| 5. |
- Rodrigues De Miranda, Joachim, et al.
(författare)
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Genetic Characterization of a Novel Iflavirus Associated with Vomiting Disease in the Chinese Oak Silkmoth Antheraea pernyi
- 2014
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Ingår i: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 9
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Tidskriftsartikel (refereegranskat)abstract
- Larvae of the Chinese oak silkmoth (Antheraea pernyi) are often affected by AVD (A. pernyi vomiting disease), whose causative agent has long been suspected to be a virus. In an unrelated project we discovered a novel positive sense single-stranded RNA virus that could reproduce AVD symptoms upon injection into healthy A. pernyi larvae. The genome of this virus is 10,163 nucleotides long, has a natural poly-A tail, and contains a single, large open reading frame flanked at the 5' and 3' ends by untranslated regions containing putative structural elements for replication and translation of the virus genome. The open reading frame is predicted to encode a 3036 amino acid polyprotein with four viral structural proteins (VP1-VP4) located in the N-terminal end and the non-structural proteins, including a helicase, RNA-dependent RNA polymerase and 3C-protease, located in the C-terminal end of the polyprotein. Putative 3C-protease and autolytic cleavage sites were identified for processing the polyprotein into functional units. The genome organization, amino acid sequence and phylogenetic analyses suggest that the virus is a novel species of the genus Iflavirus, with the proposed name of Antheraea pernyi Iflavirus (ApIV).
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| 6. |
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| 7. |
- Rodrigues De Miranda, Joachim, et al.
(författare)
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Genome Characterization, Prevalence and Distribution of a Macula-Like Virus from Apis mellifera and Varroa destructor
- 2015
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Ingår i: Viruses. - : MDPI AG. - 1999-4915. ; 7, s. 3586-3602
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Tidskriftsartikel (refereegranskat)abstract
- Around 14 distinct virus species-complexes have been detected in honeybees, each with one or more strains or sub-species. Here we present the initial characterization of an entirely new virus species-complex discovered in honeybee (Apis mellifera L.) and varroa mite (Varroa destructor) samples from Europe and the USA. The virus has a naturally poly-adenylated RNA genome of about 6500 nucleotides with a genome organization and sequence similar to the Tymoviridae (Tymovirales; Tymoviridae), a predominantly plant-infecting virus family. Literature and laboratory analyses indicated that the virus had not previously been described. The virus is very common in French apiaries, mirroring the results from an extensive Belgian survey, but could not be detected in equally-extensive Swedish and Norwegian bee disease surveys. The virus appears to be closely linked to varroa, with the highest prevalence found in varroa samples and a clear seasonal distribution peaking in autumn, coinciding with the natural varroa population development. Sub-genomic RNA analyses show that bees are definite hosts, while varroa is a possible host and likely vector. The tentative name of Bee Macula-like virus (BeeMLV) is therefore proposed. A second, distantly related Tymoviridae-like virus was also discovered in varroa transcriptomes, tentatively named Varroa Tymo-like virus (VTLV).
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| 8. |
- Rodrigues De Miranda, Joachim
(författare)
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ICTV Virus Taxonomy Profile: Polycipiviridae
- 2019
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Ingår i: Journal of General Virology. - : Microbiology Society. - 0022-1317 .- 1465-2099. ; 100, s. 554-555
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Tidskriftsartikel (refereegranskat)abstract
- Polycipiviridae is a family of picorna-like viruses with non-segmented, linear, positive-sense RNA genomes of approximately 10-12 kb. Unusually for viruses within the order Picornavirales, their genomes are polycistronic, with four (or more) consecutive 5' -proximal open reading frames (ORFs) encoding structural (and possibly other) proteins and a long 3' ORF encoding the replication polyprotein. Members of species within the family have all been detected in ants or via arthropod transcriptomic datasets. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the Polycipiviridae, which is available at www.ictv.global/report/polycipiviridae.
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| 9. |
- Rodrigues De Miranda, Joachim, et al.
(författare)
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RNAi och virusbehandling i bin.
- 2012
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Ingår i: Bitidningen. - 0006-3886. ; 10, s. 12-14
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Tidskriftsartikel (populärvet., debatt m.m.)
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| 10. |
- Rodrigues De Miranda, Joachim
(författare)
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Specific Cues Associated With Honey Bee Social Defence against Varroa destructor Infested Brood
- 2016
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Ingår i: Scientific Reports. - : Springer Science and Business Media LLC. - 2045-2322. ; 6
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Tidskriftsartikel (refereegranskat)abstract
- Social immunity forms an essential part of the defence repertoire of social insects. In response to infestation by the parasitic mite Varroa destructor and its associated viruses, honey bees (Apis mellifera L.) have developed a specific behaviour (varroa-sensitive hygiene, or VSH) that helps protect the colony from this parasite. Brood cells heavily infested with mites are uncapped, the brood killed, and the cell contents removed. For this extreme sacrifice to be beneficial to the colony, the targeting of parasitized brood for removal must be accurate and selective. Here we show that varroa-infested brood produce uniquely identifiable cues that could be used by VSH-performing bees to identify with high specificity which brood cells to sacrifice. This selective elimination of mite-infested brood is a disease resistance strategy analogous to programmed cell death, where young bees likely to be highly dysfunctional as adults are sacrificed for the greater good of the colony.
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