| 1. |
- Lohmander, L. Stefan, et al.
(författare)
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Metalloproteinases, tissue inhibitor, and proteoglycan fragments in knee synovial fluid in human osteoarthritis
- 1993
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Ingår i: Arthritis and Rheumatism. - 0004-3591. ; 36:2, s. 181-189
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Tidskriftsartikel (refereegranskat)abstract
- Objective. To determine the concentrations of human stromelysin-1, collagenase, tissue inhibitor of metalloproteinases (TIMP), and proteoglycan fragments in knee synovial fluid in patients with injury to the meniscus or anterior cruciate ligament, posttraumatic osteoarthritis, primary osteoarthritis, or pyrophosphate arthritis. Methods. Synovial fluid samples were collected from patients with knee disease diagnosed arthroscopically and radiologically. Concentrations of stromelysin-1, collagenase, and TIMP-1 were determined by sandwich immunoassay, using monoclonal and polyclonal antibodies. Fragments of cartilage proteoglycan containing the chondroitin sulfate-binding region were determined by immunoassay with a polyclonal antibody. Results. Average concentrations of metalloproteinases, TIMP, and proteoglycan fragments in joint fluid were significantly elevated in patients from all disease groups as compared with volunteers with healthy knees (reference group). Stromelysin concentrations in disease groups averaged 15-45 times that of the reference group. The molar ratios between stromelysin and collagenase varied between 10 and 150. The molar ratio between total stromelysin and free TIMP was 0.5 in the reference group and between 1.6 and 5.3 in the disease groups. Conclusion. Stromelysin concentration in joint fluid is a parameter that distinguishes diseased joints from healthy joints, with a sensitivity of 84% and a specificity of 90%. The high concentrations of metalloproteinase relative to TIMP in joint fluid from patients with the conditions studied may be associated with cartilage matrix degradation in these arthritides.
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| 2. |
- Messner, Karola, et al.
(författare)
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Cartilage mechanics and morphology, synovitis and proteoglycan fragments in rabbit joint fluid after prosthetic meniscal substitution
- 1993
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Ingår i: Biomaterials. - 0142-9612. ; 14:3, s. 163-168
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Tidskriftsartikel (refereegranskat)abstract
- The effects of meniscal substitution with a Dacron® or Teflon® prosthesis on rabbit knee-joint cartilage were studied by indentation tests, gross and histological inspection, analysis of proteoglycan fragments in joint fluid and an evaluation of synovial changes. Cartilage mechanics and cartilage morphology were similarly abnormal after meniscectomy and meniscal substitution. The elevated concentrations of proteoglycan fragments in joint fluid and the more severe synovial changes in joints with a meniscal substitute, as compared to meniscectomy, probably resulted from irritation of the artificial implant, but also reflected the remaining effects from the necessary bone drilling. In this short-term experiment, the use of an artificial meniscal substitute could not prevent cartilage degeneration after meniscectomy.
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| 3. |
- Messner, Karola, et al.
(författare)
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Proteoglycan fragments in rabbit joint fluid correlated to arthrosis stage
- 1993
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Ingår i: Acta Orthopaedica. - : Medical Journals Sweden AB. - 1745-3674 .- 0001-6470. ; 64:3, s. 312-316
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Tidskriftsartikel (refereegranskat)abstract
- Proteoglycan fragment concentrations in joint fluid of rabbit knees with various degrees of secondary arthrosis after meniscal resection, meniscal substitution, and anterior cruciate ligament transection, were related to the gross appearance of the articular cartilage. Knees with normal cartilage were distinguishable by the concentration of proteoglycan fragments in joint fluid from knees with arthrosis. We found a correlation (r 0.61) between increasing arthrosis and increasing concentrations of proteoglycan fragments.
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