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Träfflista för sökning "hsv:(MEDICIN OCH HÄLSOVETENSKAP) hsv:(Klinisk medicin) ;srt2:(1990-1999);srt2:(1993);pers:(Olovsson Matts 1958)"

Sökning: hsv:(MEDICIN OCH HÄLSOVETENSKAP) hsv:(Klinisk medicin) > (1990-1999) > (1993) > Olovsson Matts 1958

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1.
  • Olovsson, Matts, 1958-, et al. (författare)
  • Biotin labelling of chicken antibodies and their subsequent use in ELISA and immunohistochemistry
  • 1993
  • Ingår i: Comparative Immunology, Microbiology & Infectious Diseases. - 0147-9571 .- 1878-1667. ; 16:2, s. 145-152
  • Tidskriftsartikel (refereegranskat)abstract
    • Avian antibodies have many advantages to mammalian antibodies due to the phylogenetic differences between birds and mammals, resulting in an increased sensitivity and a decreased background in many immunological assays. Since the avidin-biotin system is an efficient detection system for antibodies with a high sensitivity, we wanted to investigate the activity and unspecific binding of optimally biotin labelled chicken antibodies in ELISA and immunohistochemistry. We report on the conditions for biotinylation of chicken antibodies and that optimally biotinylated antibodies show a high activity and a low background in both ELISA and immunohistochemistry.
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2.
  • Olovsson, Matts, 1958-, et al. (författare)
  • Methods to produce stage-specific monoclonal antibodies against mouse trophoblast cells by intrasplenic and in vitro immunizations.
  • 1993
  • Ingår i: Hybridoma. - 0272-457X .- 2168-7897. ; 12:3
  • Tidskriftsartikel (refereegranskat)abstract
    • Monoclonal antibodies recognizing epitopes expressed by invasive mouse trophoblast cells were obtained by immunizing male mice of the NMRI strain with mouse egg-cylinders obtained by culturing blastocysts on agarose for 4 days. Immunization was achieved by intrasplenic and in vitro methods. The procedures described outline a rational way of producing and selecting antibodies which are stage-specific for the mouse trophoblast at implantation and early placentation. Preimplantation stage-specificity was tested and adhesive blastocysts only were detected by 4 antibodies. Thus, these antibodies become markers for that developmental stage which characterizes an adhesive blastocyst ready to implant. Postimplantation stage-specificity was demonstrated by 6 antibodies, which labelled trophoblast cells of day 7 implantations only. Thus, there is a specific expression of some epitopes by the invasive trophoblast. Cell surface epitopes were detected on adhesive blastocysts by 4 antibodies and on the invasive trophoblast of the egg-cylinder by 7 antibodies. Shedded antigens from egg-cylinders in vitro were detected by 5 antibodies. Four of these antibodies detected substances released only from early implantation stages. A functional effect was demonstrated for an IgM antibody which impaired hatching and outgrowth of blastocysts in vitro.
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3.
  • Olovsson, Matts, 1958-, et al. (författare)
  • Structural and functional properties of trophoblast cells of mouse egg-cylinders in vitro.
  • 1993
  • Ingår i: Anatomical Record. - : Wiley. - 0003-276X .- 1097-0185. ; 236:2
  • Tidskriftsartikel (refereegranskat)abstract
    • Embryos at the egg-cylinder stage were obtained by culturing blastocysts in vitro on an agarose surface for 4 days. The adhesiveness and outgrowth activity of the trophoblast of the egg-cylinder were compared with those properties of the flushed adhesive blastocyst. Trophoblast cells of egg-cylinders were found to be more adhesive and their outgrowth activity greater than in the case of trophoblast cells of adhesive blastocysts. The cultured egg-cylinders presented two subpopulations of giant trophoblast cells. They represent probably two stages of differentiation of the same trophoblast population. The most distinctive morphological differences of these subpopulations were that the surface of one was covered with small blebs and the cytoplasm had relatively few ribosomes, while the surface of the other subpopulation was covered with large blebs and microvilli and the cytoplasm was rich in ribosomes and large, dense granules. The two types of giant trophoblast cells of the day 7 implantation site consist of one subpopulation whose cytoplasm has few organelles, and the other subpopulation, whose cytoplasm is rich in ribosomes, lysosome-like bodies, and heterophagosomes. Hence, we conclude that the in vitro grown trophoblast cells have counterparts in the in vivo implanting embryos. The lectin binding pattern of the agarose cultured egg-cylinder trophoblast cells was similar to that of the adhesive and/or invasive trophoblast cells grown in vivo. Thus, the in vitro grown egg-cylinders are appropriate material for the analysis of trophoblast cells at the invasive stage of implantation.
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  • Resultat 1-3 av 3
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refereegranskat (3)
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Nilsson, B O (2)
Larsson, Anders (1)
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Engelska (3)
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Medicin och hälsovetenskap (3)
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