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Influence of Glycos...
Influence of Glycosylation on Interfacial Properties of Recombinant Mucins : Adsorption, Surface Forces, and Friction
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- An, Junxue (författare)
- KTH,Yt- och korrosionsvetenskap
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- Jin, Chunsheng (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Dedinaite, Andra (författare)
- KTH,RISE,Kemi och material,KTH Royal Institute of Technology, Sweden,Yt- och korrosionsvetenskap,SP Technical Research Institute of Sweden
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- Holgersson, Jan (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för klinisk kemi och transfusionsmedicin,Institute of Biomedicine, Department of Clinical Chemistry and Transfusion Medicine
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- Karlsson, Niclas G., 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
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- Claesson, Per Martin (författare)
- KTH,RISE,Kemi och material,KTH Royal Institute of Technology, Sweden,Yt- och korrosionsvetenskap,SP Technical Research Institute of Sweden
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(creator_code:org_t)
- 2017-04-26
- 2017
- Engelska.
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Ingår i: Langmuir. - : American Chemical Society. - 0743-7463 .- 1520-5827. ; 33:18, s. 4386-4395
- Relaterad länk:
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http://kth.diva-port...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://gup.ub.gu.se...
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Abstract
Ämnesord
Stäng
- Interfacial properties of two brush-with-anchor mucins, C-P55 and C-PSLex, have been investigated at the aqueous solution/poly(methyl methacrylate) (PMMA) interface. Both are recombinant mucin-type fusion proteins, produced by fusing the glycosylated mucin part of P-selectin glycoprotein ligand-1 (PSLG-1) to the Fc part of a mouse immunoglobulin in two different cells. They are mainly expressed as dimers upon production. Analysis of the O-glycans shows that the C-PSLex mucin has the longer and more branched side chains, but C-P55 has slightly higher sialic acid content. The adsorption of the mucins to PMMA surfaces was studied by quartz crystal microbalance with dissipation. The sensed mass, including the adsorbed mucin and water trapped in the layer, was found to be similar for these two mucin layers. Atomic force microscopy with colloidal probe was employed to study surface and friction forces between mucin-coated PMMA surfaces. Purely repulsive forces of steric origin were observed between mucin layers on compression, whereas a small adhesion was detected between both mucin layers on decompression. This was attributed to chain entanglement. The friction force between C-PSLex-coated PMMA is lower than that between C-P55-coated PMMA at low loads, but vice versa at high loads. We discuss our results in terms of the differences in the glycosylation composition of these two mucins.
Ämnesord
- TEKNIK OCH TEKNOLOGIER -- Materialteknik -- Metallurgi och metalliska material (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Materials Engineering -- Metallurgy and Metallic Materials (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Klinisk laboratoriemedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Clinical Laboratory Medicine (hsv//eng)
Nyckelord
- Atomic force microscopy
- Chains
- Esters
- Glycosylation
- Proteins
- Recombinant proteins
- Solutions
- Tribology
- Branched side chains
- Chain entanglements
- Colloidal probes
- Interfacial property
- Methyl methacrylates
- P-selectin glycoprotein ligand-1
- Quartz crystal microbalance with dissipation
- Repulsive forces
- Friction
- bovine submaxillary mucin
- porcine gastric mucin
- hydrophobic surfaces
- fusion proteins
- microscope cantilevers
- boundary lubrication
- polymer
- oligosaccharides
- glycoprotein
- lubricin
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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