| 1. |
- Campbell, Kate, 1987, et al.
(författare)
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Self-Establishing Communities: A Yeast Model to Study the Physiological Impact of Metabolic Cooperation in Eukaryotic Cells
- 2019
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Ingår i: Methods in Molecular Biology. - New York, NY : Springer New York. - 1940-6029 .- 1064-3745. ; 2049, s. 263-282
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- All biosynthetically active cells are able to export and import metabolites, the small molecule intermediaries of metabolism. In dense cell populations, this hallmark of cells results in the intercellular exchange of a wide spectrum of metabolites. Such metabolite exchange enables metabolic specialization of individual cells, leading to far reaching biological implications, as a consequence of the intrinsic connection between metabolism and cell physiology. In this chapter, we discuss methods on how to study metabolite exchange interactions by using self-establishing metabolically cooperating communities (SeMeCos) in the budding yeast Saccharomyces cerevisiae. SeMeCos exploit the stochastic segregation of episomes to progressively increase the number of essential metabolic interdependencies in a community that grows out from an initially prototrophic cell. By coupling genotype to metabotype, SeMeCos allow for the tracking of cells while they specialize metabolically and hence the opportunity to study their progressive change in physiology.
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| 2. |
- Jesorka, Aldo, 1967, et al.
(författare)
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Microfluidic technology for investigation of protein function in single adherent cells
- 2019
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Ingår i: Methods in Enzymology. - : Elsevier. - 1557-7988 .- 0076-6879. ; 628
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Bokkapitel (övrigt vetenskapligt/konstnärligt)abstract
- Instrumental techniques and associated methods for single cell analysis, designed to investigate and measure a broad range of cellular parameters in search of unique features, address key limitations of conventional cell-based assays with their ensemble average response. While many different single cell techniques exist for suspension cultures, which can process and characterize large numbers of individual cells in rapid succession, the access to surface-immobilized cells in typical 2D and 3D culture environments remains challenging. Open space microfluidics has created new possibilities in this area, allowing for exclusive access to single cells in adherent cultures, even at high confluency. In this chapter, we briefly review new microtechnologies for the investigation of protein function in single adherent cells, and present an overview over related recent applications of the multifunctional pipette (Biopen), a microfluidic multi-solution dispensing system that uses hydrodynamic confinement in open volume environments in order to establish a superfusion zone over selected single cells in adherent cultures.
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| 3. |
- Pless, Christian J., et al.
(författare)
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Emerging strategies in 3D printed tissue models for in vitro biomedical research
- 2022
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Ingår i: Bioprinting : From Multidisciplinary Design to Emerging Opportunities - From Multidisciplinary Design to Emerging Opportunities. - 9780323854306 - 9780323854313 ; , s. 207-246
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Bokkapitel (refereegranskat)abstract
- 3D bioprinting has the potential to provide a unified framework for the manufacturing of tissue models for biomedical research, including drug discovery, disease modeling, and regenerative medicine. However, it remains challenging to 3D print replicas of human tissues that have accurate cell types, cellular densities, extracellular matrix compositions, and that can be assayed in a minimally invasive manner for chronic studies. Here, we review how recent breakthroughs in stem cell biology, tissue engineering, and materials science have led to novel 3D printing strategies that have the potential to solve these challenges.
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| 4. |
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| 5. |
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| 6. |
- Jergil, Bengt, et al.
(författare)
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Purification of plasma membranes by affinity partitioning
- 2000
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Ingår i: Aqueous Two-Phase Systems : Methods and Protocols - Methods and Protocols. - New Jersey : Humana Press. - 1940-6061. - 9780896035416 - 9781592590285 ; 11, s. 193-200
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 7. |
- Katona, Borbala, et al.
(författare)
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The Human Protein Atlas and Antibody-Based Tissue Profiling in Clinical Proteomics
- 2022
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Ingår i: Clinical Proteomics. - New York, NY : Humana Press. - 9781071619353 - 9781071619360 ; , s. 191-206
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Bokkapitel (refereegranskat)abstract
- Immunohistochemistry (IHC) is a standard method for spatial proteomics and allows for exploration of protein expression at single-cell resolution within the intact tissue environment. Stringent procedures and proper antibody validation strategies are however needed to ensure reliability of results. Application-specific strategies have been proposed by the scientific community to ensure high quality despite variations in sample preparation between different antibody-based methods. Here, the entire workflow utilized within the Human Protein Atlas, from sample preparation to annotation of the IHC staining patterns is described in detail, with important notes on various factors that can affect the outcome of IHC. Methods include tissue microarray (TMA) production, tissue sectioning, IHC, annotation, and validation. Also, building on previously suggested validation strategies, IHC-specific orthogonal and independent validation methods are outlined.
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| 8. |
- Sobti, R. C., et al.
(författare)
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Preface
- 2023
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Ingår i: Biomedical Research, Medicine, and Disease. - 9781003220404 - 9781000811193
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Bokkapitel (övrigt vetenskapligt/konstnärligt)
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| 9. |
- von Zur-Mühlen, Bengt, Docent, 1966-, et al.
(författare)
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Treating diabetes with islet transplantation : Lessons learnt from the Nordic network for clinical islet transplantation
- 2019. - 1
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Ingår i: Transplantation, Bioengineering, and Regeneration of the Endocrine Pancreas. - : Academic Press. - 9780128148334 ; , s. 599-611
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Bokkapitel (refereegranskat)abstract
- In this chapter we present the Nordic experiences withclinical islet transplantation after starting networks. TheNordic Network for Clinical Islet Transplantation (NNCIT)was established in 2000. In addition, we also present a localmultidisciplinary effort organized as part of a Centerof Excellence (CoE) in Type 1 Diabetes. Even though careof type 1 diabetic patients has improved over time thereare still patients who have substantial problems withhypoglycemic incidents, unawareness, and glycemiclability and need treatment other than injected exogenousinsulin.1 In the Nordic countries it is now consideredas innovative rather than an experimental therapy.Islet transplantation still has limitations, only making ita treatment suitable for a small part of type 1 diabeticpatients. The main limitations are the need of immunosuppressanttherapy (as in all organ transplants betweenindividuals), early loss of substantial amounts of transplantedislets, and a limited source of islets (deceasedorgan donors). To have a favorable clinical result there isoften a need for retransplantation and or complementarytherapies. Islet transplantation as such has great potentialin the near future for modification, encapsulation ofthe islets, or stem cell therapies.2,3 Experiences made sofar will be useful when this new era evolves. Even if islettransplantation is a minimally invasive and surgicallysimple technique there are technically complex partssuch as islet isolation that require highly specialized staffand a need for multidisciplinary expertise, which is whyit for the foreseeable future will continue to be centralizedto a few units to ensure high quality and continueddevelopment. The aim of our collaborations, local,regional, and international, is to offer the best treatmentfor those patients for whom conventional treatment isinadequate and take advantage of the expertise distributedin several different disciplines.
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