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Aili, Daniel, 1977-, et al.
(author)
Controlled Assembly of Gold Nanoparticles using De Novo Designed Polypeptide Scaffolds
2008
In: Proceedings SPIE, Vol. 6885, Photonic Biosensing and Microoptics. - : SPIE. ; , s. 688506-1-688506-8
Conference paper (peer-reviewed) abstract
Heterodimerization between designed helix-loop-helix polypeptides was utilized in order to assemble gold nanoparticles on planar substrates. The peptides were designed to fold into four-helix bundles upon dimerization. A Cys-residue in the loop region was used to immobilize one of the complementary peptides on a maleimide containing SAM on planar gold substrates whereas the second peptide was immobilized directly on gold nanoparticles. Introducing the peptide decorated particles over a peptide functionalized surface resulted in particle assembly. Further, citrate stabilized particles were assembled on amino-silane modified glass and silicon substrates. By subsequently introducing peptides and gold nanoparticles, particle-peptide hybrid multi layers could be formed.
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Backlund, Per, et al.
(author)
Pre-hospital training and simulation initiative
2014
Conference paper (peer-reviewed) abstract
Background The pre-hospital process is a complex one involving aspects such as medical skills as well as care taking, team performance, inter-organizational cooperation and communication. This calls for novel training methods and technology support. Our review of literature (covering the areas of pre-hospital care, training simulator technologies and methods and process modelling) indicates that the different aspects are typically trained in isolation, e.g. medical skills using patient simulators.Objective The pre-hospital training center project addresses the overall complexity of the pre-hospital process by taking all of the aspects into account when designing scenarios and technology support for training the complete prehospital process (covering alarm, on-scene activities, transportation and hand-over). This is indeed a challenging task as we need to develop both training methods and technology support for a very complex training situation.Methods The project will develop a prototype scenario along with technology support to enact it. The training scenario will involve many of the aspects listed above and will be tested in a field experiment with ambulance personnel. Results The expected outcome of the project is a platform for establishing a pre-hospital simulation and training center. The initial technologies, research results and experiences will be used to form a consortium for further work and development. Conclusions We have identified a need for a pre-hospital training center with the unique and ambitious idea of covering the entire pre-hospital process as well as its many interacting aspects. To the best of our knowledge this approach is not at all common and we expect the complexity to be so high that it is a challenging enough research area that can only be addressed if we have a well-designed simulation and training center in place with all the different areas of knowledge represented, i.e. pre-hospital medicine as well as simulation and visualization technology.
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Bry, Maija, et al.
(author)
Insulin-like growth factor-I (IGF-I) aggravates lung injury in a mouse model of bronchopulmonary dysplasia
2015
In: Pediatric Academic Societies Annual Meeting.
Conference paper (other academic/artistic) abstract
Background: Trials using continuous intravenous administration of insulin-like growth factor (IGF)-I to preterm infants for the prevention of retinopathy of prematurity (ROP) are currently underway (Ley et al., 2013). However, the effects of exogenous IGF-I on developing organs of the newborn are unknown. IGF-I has been shown to play a critical role in the pathogenesis of asthma (Lee et al., 2014). In addition, IGF-I levels are high in the lungs of infants with bronchopulmonary dysplasia (BPD) (Capoluongo et al., 2008). We have previously shown that perinatal pulmonary IL-1b expression causes a BPD-like lung disease in newborn mice. Objective: To study the effects of systemic postnatal administration of IGF-I on lung inflammation and morphogenesis in a murine model of BPD. Methods: IL-1b production in pulmonary epithelium of transgenic fetuses and pups was induced by doxycycline administration (0.5 mg/ml) in drinking water to pregnant and lactating dams from the beginning of pregnancy until sacrifice of the pups on postnatal day (PN) 7. Three intraperitoneal injections of recombinant human IGF-I (1 μg/g body weight) or an equal volume of PBS were administered to transgenic IL-1β-expressing mice and their littermate controls on PN 2, 4 and 6. Lung inflammation and structure were studied on PN 7. Results: IGF-I significantly increased both neutrophil and macrophage infiltration in the lungs of IL-1b-expressing infant mice, and also significantly increased the number of neutrophils in the lungs of littermate controls not expressing IL-1β. In addition, IGF-I increased alveolar septal wall thickness in IL-1b-expressing mice, indicating that IGF-I potentiated IL-1b-induced lung injury. Conclusions: IGF-I aggravated lung inflammation and injury in the newborn lung, indicating that IGF-I administration may have deleterious effects on lung development in preterm infants at risk of BPD.
