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Sökning: hsv:(TEKNIK OCH TEKNOLOGIER) hsv:(Industriell bioteknik) > Mälardalens universitet

  • Resultat 1-10 av 46
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1.
  • de Maré, Lena, et al. (författare)
  • A cultivation technique for E. coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor
  • 2005
  • Ingår i: Biotechnology Letters. - : Springer Science and Business Media LLC. - 0141-5492 .- 1573-6776. ; 27:14, s. 983-990
  • Tidskriftsartikel (refereegranskat)abstract
    • A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.
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2.
  • Nordlander, Eva, et al. (författare)
  • Investigating the possibility of applying an ADM1 based model to a full-scale co-digestion plant
  • 2017
  • Ingår i: Biochemical engineering journal. - : Elsevier. - 1369-703X .- 1873-295X. ; 120, s. 73-83
  • Tidskriftsartikel (refereegranskat)abstract
    • This study investigated the possibility of using a model based on the anaerobic digestion model no. 1 (ADM1) on a full-scale 4000 m3 digester in order to understand how such theoretical models can be applied to a real industrial process. The industrial scale digester co-digests the organic fraction of municipal solid waste, grease trap sludge, and ley crop silage with varying feed rates and amounts of volatile solids. A year of process data was collected. Biogas flow, methane content/flow, and ammonia nitrogen were the variables that the model was best at predicting (index of agreement at 0.78, 0.61/0.77, and 0.68, respectively). The model was also used to investigate the effect of increasing the volatile solids (VS) concentration entering the digester. According to simulation results, increasing the influent VS concentration will increase biogas and methane outflow (from 1.5 million Nm3 methane to more than 2 million Nm3 methane), but decrease the amounts of biogas/methane per unit of volatile solids (from about 264 Nm3 methane per tonne VS to below 215 Nm3 methane per tonne VS).
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3.
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4.
  • Skvaril, Jan, 1982-, et al. (författare)
  • Applications of near-infrared spectroscopy (NIRS) in biomass energy conversion processes : A review
  • 2017
  • Ingår i: Applied spectroscopy reviews (Softcover ed.). - : Informa UK Limited. - 0570-4928 .- 1520-569X. ; 52:8, s. 675-728
  • Forskningsöversikt (refereegranskat)abstract
    • Biomass used in energy conversion processes is typically characterized by high variability, making its utilization challenging. Therefore, there is a need for a fast and non-destructive method to determine feedstock/product properties and directly monitor process reactors. The near-infrared spectroscopy (NIRS) technique together with advanced data analysis methods offers a possible solution. This review focuses on the introduction of the NIRS method and its recent applications to physical, thermochemical, biochemical and physiochemical biomass conversion processes represented mainly by pelleting, combustion, gasification, pyrolysis, as well as biogas, bioethanol, and biodiesel production. NIRS has been proven to be a reliable and inexpensive method with a great potential for use in process optimization, advanced control, or product quality assurance.
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5.
  • Naqvi, Muhammad, 1983-, et al. (författare)
  • Bio-refinery system in a pulp mill for methanol production with comparison of pressurized black liquor gasification and dry gasification using direct causticization
  • 2012
  • Ingår i: Applied Energy. - : Elsevier. - 0306-2619 .- 1872-9118. ; 90:1, s. 24-31
  • Tidskriftsartikel (refereegranskat)abstract
    • Black liquor gasification (BLG) for bio-fuel or electricity production at the modern pulp mills is a field in continuous evolution and the efforts are considerably driven by the climate change, fuel security, and renewable energy. This paper evaluates and compares two BLG systems for methanol production: (i) oxygen blown pressurized thermal BLG; and (ii) dry BLG with direct causticization, which have been regarded as the most potential technology candidates for the future deployment. A key objective is to assess integration possibilities of BLG technologies with the reference Kraft pulp mill producing 1000 air dried tonnes (ADt) pulp/day replacing conventional recovery cycle. The study was performed to compare the systems’ performance in terms of potential methanol production, energy efficiency, and potential CO2 reductions. The results indicate larger potential of black liquor conversion to methanol from the pressurized BLG system (about 77 million tonnes/year of methanol) than the dry BLG system (about 30 million tonnes/year of methanol) utilizing identical amount of black liquor available worldwide (220 million tDS/year). The potential CO2 emissions reduction from the transport sector is substantially higher in pressurized BLG system (117 million tonnes/year CO2 reductions) as compared to dry BLG system (45 million tonnes/year CO2 reductions). However, the dry BLG system with direct causticization shows better results when considering consequences of additional biomass import. In addition, comparison of methanol production via BLG with other bio-refinery products, e.g. hydrogen, dimethyl ether (DME) and bio-methane, has also been discussed.
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6.
  • Sundström, Heléne, et al. (författare)
  • Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
  • 2004
  • Ingår i: Biotechnology Letters. - 0141-5492 .- 1573-6776. ; 26:19, s. 1533-1539
  • Tidskriftsartikel (refereegranskat)abstract
    • In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.
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7.
  • Waara, Sylvia, et al. (författare)
  • Performance of a constructed wetland system for treatment of landfill leachate.
  • 2008
  • Ingår i: Waste 2008. Waste and Resource management-A shared responsibility. - Stanton-on-the-Wolds : Waste Conference Ltd.. ; , s. 655-667, s. 655-667
  • Konferensbidrag (refereegranskat)abstract
    • The performance of a constructed wetland for treatment of landfill leachate has been evaluated based upon data obtained during 4 years (2003-2006). It consists of a series of 10 ponds with a total capacity of 52.000 m3 covering 8 ha. Using univariate and multivariate statistics (PCA) the reduction pattern of a large number of chemical parameters including heavy metals has been investigated in 3 parts of the wetland with equal volume. Analyses show that many parameters are removed to the greatest extent in the first part of the system (e.g. many heavy metals, total suspended solids) or the second part of the system (N-NH4) while other parameters such as total nitrogen are more gradually reduced (10 ton/year removed).  Toxicity testing with 5 bioassays showed that toxicity was sometimes observed at the inlet but no toxicity was observed at the outlet for 4 of the test species. The data presented will be used for optimizing the treatment process as well as to improve the monitoring program.
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8.
  • Wållberg, Fredrik, et al. (författare)
  • Monitoring and quantification of inclusion body formation in Escherichia coli by multi-parameter flow cytometry
  • 2005
  • Ingår i: Biotechnology Letters. - : Springer Science and Business Media LLC. - 0141-5492 .- 1573-6776. ; 27:13, s. 919-926
  • Tidskriftsartikel (refereegranskat)abstract
    • Multi-parameter flow cytometry was used to monitor the formation of promegapoietin (PMP) inclusion bodies during a high cell density Escherichia coli fed-batch fermentation process. Inclusion bodies were labelled with a primary antibody and then with a secondary fluorescent antibody. Using this method it was possible to detect PMP inclusion body formation with a high specificity and it was possible to monitor the increased accumulation of the protein with process time (6-48 mg PMP/g CDW) whilst highlighting population heterogeneity.
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9.
  • Johansson, LarsErik, et al. (författare)
  • Determination of conductivity of bacteria by using cross-flow filtration
  • 2006
  • Ingår i: Biotechnology letters. - : Springer Science and Business Media LLC. - 0141-5492 .- 1573-6776. ; 28:8, s. 601-603
  • Tidskriftsartikel (refereegranskat)abstract
    • An important property of the bacterial surface is its conductivity. To obtain reliable conductivity values, it is essential to handle the cells as gently as possible during the measurement procedure. We have developed a method where a standard conductivity meter is used in combination with cross-flow filtration, thus avoiding repeated centrifugation and resuspension. With this method, the conductivity of Bacillus subtilis was determined to be 7000 mu S/cm, which is a deviation from previously published data by almost an order of a magnitude.
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10.
  • Kanders, Linda, 1979-, et al. (författare)
  • Impact of seeding on the start-up of one-stage deammonification MBBRs
  • 2014
  • Ingår i: Environmental technology. - : Taylor & Francis. - 0959-3330 .- 1479-487X. ; 35:22, s. 2767-2773
  • Tidskriftsartikel (refereegranskat)abstract
    • Treating nitrogen-rich reject water from anaerobically digested sludge with deammonification has become a very beneficial side stream process. One common technique is the one-stage moving bed bioreactors (MBBRs), which in comparison with the other deammonification techniques can be started up without seeding anammox bacteria. This study investigated the impact of biofilm seeding on the start-up of one-stage deammonification MBBRs. Two lab-scale reactors were run in parallel with partial nitritation for 56 days until 11% of the carrier area in one reactor was replaced with fully developed deammonification biofilm to work as the seeding material. The seeded reactor started nitrogen reduction immediately up to a plateau of 1.3gNm−2 d−1; after another 54 days on day 110, the reduction significantly increased. At the same time, the non-seeded reactor also started to reduce nitrogen due to deammonification. The development was followed with both nitrogen analyses and fluorescence in situ hybridization analyses. On day 134, the biofilm in both reactors contained >90% anammox bacteria and reached maximum nitrogen removal rates of 7.5 and 5.6gNm−2 d−1 in the seeded and non-seeded reactor, respectively. Over 80% of the inorganic nitrogen was reduced. In conclusion, the seeding did not contribute to a shorter start-up time or the achieved anammox enrichment, although it did contribute to a partial, immediate nitrogen reduction. The boundary conditions are the most important factors for a successful start-up in a deammonification MBBR system.
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