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- Porras, Ana Maria, et al.
(författare)
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Chemical micropatterning of hyaluronic acid hydrogels for controlled cell adhesion
- 2018
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- The blood brain barrier is constituted by endothelial cells, astrocytes and pericytes; and are organized into well structured units [1]. Standard cell culture techniques cannot recapitulate this organized structure. Hydrogels are an attractive scaffold due to their mechanical and chemical properties similar to those in body tissue[2] We propose the use of a photo-crosslinkable hyaluronic acid hydrogel as cell culture scaffold. Furthermore, chemical cues can be added into the hydrogel matrix to promote and control cell adhesion using UV lithography.
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- Evander, Mikael, et al.
(författare)
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Microfluidic PMMA interfaces for rectangular glass capillaries
- 2014
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Ingår i: Journal of Micromechanics and Microengineering. - : IOP Publishing. - 0960-1317 .- 1361-6439. ; 24:2
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Tidskriftsartikel (refereegranskat)abstract
- We present the design and fabrication of a polymeric capillary fluidic interface fabricated by micro-milling. The design enables the use of glass capillaries with any kind of cross-section in complex microfluidic setups. We demonstrate two different designs of the interface; a double-inlet interface for hydrodynamic focusing and a capillary interface with integrated pneumatic valves. Both capillary interfaces are presented together with examples of practical applications. This communication shows the design optimization and presents details of the fabrication process. The capillary interface opens up for the use of complex microfluidic systems in single-use glass capillaries. They also enable simple fabrication of glass/polymer hybrid devices that can be beneficial in many research fields where a pure polymer chip negatively affects the device's performance, e.g. acoustofluidics.
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- Quan, Xueling, et al.
(författare)
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In-situ monitoring of potential enhanced DNA related processes using electrochemical quartz crystal microbalance with dissipation (EQCM-D)
- 2014
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Ingår i: Electrochemistry Communications. - : Elsevier BV. - 1388-2481 .- 1873-1902. ; 48, s. 111-114
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Tidskriftsartikel (refereegranskat)abstract
- The effect of applied potential pulses on DNA functionalization (thiolated single stranded DNA) and hybridization processes has been monitored in-situ on gold surfaces using electrochemical quartz crystal microbalance with dissipation (EQCM-D). The applied potentials were chosen with respect to the potential of zero charge (E-pzc) of the gold surfaces: a positive potential to attract the negatively charged DNA molecules and a negative potential to enhance the vertical alignment due to electrostatic repulsion. The obtained results clearly show that both DNA modification and hybridization are strongly enhanced by applying potential pulses. Based on the EQCM-D results, we present a model to explain the influence of the potential pulsing. Aside from the effect of applied potentials on DNA related processes, this work also demonstrates the versatility of the combination of electrochemistry and quartz crystal microbalance with dissipation in facilitating real-time in situ monitoring of such processes. (C) 2014 Elsevier B.V. All rights reserved.
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- Tenje, Maria, et al.
(författare)
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Cantilever-based sensors
- 2016
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Ingår i: Optochemical Nanosensors. - 9781439854907 - 9781439854891 ; , s. 119-145
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Bokkapitel (refereegranskat)
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| 8. |
- Tenje, Maria, et al.
(författare)
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Cantilver based sensors
- 2013
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Ingår i: Optochemical Nanosensors. - : CRC Press. - 9781439854891 - 9780429063169
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Bokkapitel (refereegranskat)
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| 9. |
- Tenje, Maria
(författare)
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Fabrication of cantilever chip with complementary micro channel system in SU-8 for biochemical detection
- 2004
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Licentiatavhandling (övrigt vetenskapligt/konstnärligt)abstract
- This thesis presents the works on fabrication and measurements done with micro cantilevers and a micro channel system, fabricated in the photo-resist SU-8. SU-8 is a polymer patterned by UV lithography which offers a cheap and fast processing and is an interesting alternative to standard Si techniques. For the structures intended here, it proved to be a well suited material and promising results were obtained. The project involved the design of one cantilever chip with arrays of cantilevers structured into three sections of five cantilevers each and one micro channel chip, which were batch fabricated. The cantilevers are used for biochemical detection by surface stress measurements with optical read-out. Together, the two chips can be used for specific molecular detection for research oriented or medical purposes. The cantilevers proved to be very sensitive and DNA immobilisation detection was achieved. The channels are an essential component in this system for easy handling of the solutions to be investigated and sensitising of the cantilevers prior to the measurements. To achieve the above results, several experiments were performed, investigating the surface properties of SU-8. Little has until this point been known about the surface properties of the the photo-resist and several key pieces of information were obtained, which proved to be crucial in the project. Here, the work involved in the design and fabrication of both chips is described in detail. Also, the work on the surface investigations is presented and indications of necessary further work is given. A brief description of the measurements done is also included in the Thesis.
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| 10. |
- Atif, Abdul Raouf, 1996-, et al.
(författare)
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Evaluation of Ionic Interactions of Bone Cement-on-Chip
- 2019
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Konferensbidrag (övrigt vetenskapligt/konstnärligt)abstract
- INTRODUCTION: Biomaterials are synthetic materials that can be incorporated into the body to replace an impaired physiological function. Apatite calcium phosphate cements (CPCs), used for bone regeneration, give calcium-deficient hydroxyapatite (CDHA) as an end-product after a dissolution-precipitation reaction during fabrication. CDHA has a tendency to uptake calcium and release phosphate into cell culture medium. Potentially, this leads to depletion of calcium ions in solution, which can be detrimental to cell survival. The aim of this work is to embed CDHA in a microfluidic system and evaluate ion exchange at different flow rates.METHODS: CPC paste was cast into a 0.8mm pocket within a Polydimethylsiloxane (PDMS, cured at 60°C for 2h) mould. CPCs were set in 0.9% w/v NaCl at 37°C for 10 days resulting in CDHA. The PDMS containing the CDHA was then bonded to glass, leaving a 0.5mm channel gap. Minimum Essential Media (MEM, 1ml) was pumped through the channel at low (2µl/min), medium (8µl/min) and high (14µl/min) flow rates. A CDHA disc (ø=15mm, h=2mm) was immersed in MEM (1ml) at static conditions (0µl/min) for 24h. Stock Media was taken as control. Calcium and phosphorus concentrations were analysed using Inductively Coupled Plasma Optical Emission Spectroscopy.RESULTS & CONCLUSIONS: CDHA was successfully embedded in a microfluidic chip (Fig. 1A). Observed [Ca] and [P] levels were closer to levels in stock MEM at higher flow rates (Fig. 1B). We anticipate that osteoblast viability will improve when grown under flow, as opposed to static conditions, due to continuous replenishment of cell medium.
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