| 11. |
- Lindholm, Clas-Ebbe, et al.
(författare)
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Microwave-induced hyperthermia and radiotherapy in human superficial tumours: clinical results with a comparative study of combined treatment versus radiotherapy alone
- 1987
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Ingår i: International Journal of Hyperthermia. - : Informa UK Limited. - 0265-6736 .- 1464-5157. ; 3:5, s. 393-411
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Tidskriftsartikel (refereegranskat)abstract
- Eighty-five evaluable superficial recurrent malignant tumours, mainly adenocarcinomas (78 per cent), in 38 patients were treated with either combined local hyperthermia (41-45 degrees C for four sessions) and low dose radiotherapy (30.0 Gy) or the same low dose radiotherapy alone. The treatment was given for two weeks. Hyperthermia was induced externally with 2450 MHz or 915 MHz microwaves. Totally 57 tumours were given combined treatment with a complete and partial response rate of 46 and 30 per cent, respectively (duration 1-38 months). In 18 patients with 2-10 superficial tumours each, 56 tumours were used in a comparative study, comparing the effect of combined hyperthermia and low dose radiotherapy versus the same low dose radiotherapy alone, the patients acting as their own controls. The total response rates were 89 and 50 per cent, respectively, in the two treatment modality groups. The difference in response rates is significant (p = 0.0039) in favour of the combined treatment, and this is also found when comparing complete remissions only (p = 0.0027). Local pain and normal tissue reactions presented problems during and after 2450 MHz microwave-induced hyperthermia treatment, performed without a coupling water bag system. Introduction of 915 MHz microwave-induced hyperthermia with a coupling deionized water bag system and refinement of microwave applicators, as well as the temperature control system considerably reduced these problems.
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| 12. |
- Andersson, P. S., et al.
(författare)
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TISSUE CHARACTERIZATION USING LASER-INDUCED FLUORESCENCE.
- 1987
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Ingår i: Conference on Lasers and Electro-Optics. - 0936659491 ; 14, s. 46-48
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Konferensbidrag (refereegranskat)abstract
- In cancer tumor detection, tissue autofluorescence and characteristic features of injected hematoporphyrin derivative (HPD) can be utilized. The authors have studied the importance of the excitation wavelength for the achievable contrast between tumor and surrounding tissue using a rat tumor model. They have also compared the relative merits of two HPD preparations for tumor fluorescence detection. The real-time capability of the fluorescence technique is discussed.
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| 13. |
- Andersson, S., et al.
(författare)
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Tumour Localization by Means of Laser-Induced Fluorescence in Hematoporphyrin Derivative (HPD) — Bearing Tissue
- 1985
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Ingår i: Laser Spectroscopy VII : Proceedings of the Seventh International Conference, Hawaii, June 24-28, 1985 - Proceedings of the Seventh International Conference, Hawaii, June 24-28, 1985. - Berlin, Heidelberg : Springer Berlin Heidelberg. - 9783662152539 - 9783540396642 ; , s. 401-406
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Konferensbidrag (refereegranskat)abstract
- Following systemic injection, hematoporphyrin derivative (HPD) is known to be selectively retained in malignant tissue. This property can be used in a two-fold way: a) for localizing tumours by observing the characteristic dual-peaked laser-induced fluorescence from HPD, and b) for photodynamic therapy (HPD-PDT) using a localized HPD-assisted singlet oxygen release induced by irradiation of 630 nm laser light. This rapidly developing field has recently been reviewed by DOUGHERTY.
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| 14. |
- Ankerst, Jaro, et al.
(författare)
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Adenovirous type 9-induced tumorigenesis in the rat mammary gland related to sex hormonal state
- 1989
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Ingår i: Journal of the National Cancer Institute. - : Oxford University Press (OUP). - 0027-8874 .- 1460-2105. ; 81:4, s. 294-298
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Tidskriftsartikel (refereegranskat)abstract
- Adenovirus type 9 was inoculated sc into newborn Wistar/Furth rats, divided into four groups: (1) six male rats, not treated further; (2) 11 male rats, castrated at 4 weeks of age; (3) 12 male rats, castrated at 4 weeks of age and subsequently treated repeatedly with estrogen; and (4) 12 female rats, not treated further. All of the rats in group 3 developed mammary hyperplasia and tumors (fibroadenomas and lipomas), in some cases with malignant histologic structure. Rats in group 4 developed similar mammary tumors, but with later appearance and significantly slower growth. A fifth group of rats, not virus inoculated but castrated and estrogen treated as in group 3, did not develop any demonstrable mammary lesions. The results show that the effects of the virus on the mammary gland are dependent upon an estrogenic background, which by itself cannot cause tumor development in males. It is suggested that viral DNA is incorporated into the cellular DNA in such a way that it influences the synthesis and/or activity of steroid receptors, triggering tumor development.
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| 15. |
- Ankerst, J., et al.
(författare)
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SPECTRAL CHARACTERISTICS IN TISSUE DIAGNOSTICS USING LASER-INDUCED FLUORESCENCE.
- 1985
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Ingår i: Proceedings of the Medicine & Biology Symposium, ICALEO '84. - 0912035250 ; 43-48, s. 52-60
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Konferensbidrag (refereegranskat)abstract
- Studies of laser-induced fluorescence in rat tissues, including cancer tumors have been performed. Contrast enhancement techniques have been developed which improve the ability to localize a tumor when using fluorescence from the tumor-seeking substance hematoporphyrin derivative (HPD). A multi-color fluorescence imaging technique has been tested. A discussion of clinical applications is included.
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| 16. |
- Ankerst, J., et al.
(författare)
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Use of microcalorimetry in analysing the kinetics of ADCC
- 1986
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Ingår i: Journal of Immunological Methods. - : Elsevier BV. - 0022-1759. ; 88:2, s. 259-264
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Tidskriftsartikel (refereegranskat)abstract
- Microcalorimetry was found to be a useful technique for the demonstration of antibody-dependent cellular cytotoxicity (ADCC) against human melanoma cells mediated by a heterologous rabbit antiserum and two monoclonal antibodies in combination with human peripheral blood lymphocytes as effector cells. The rabbit antiserum and the monoclonal IgG3 antibody 2B2 directed against the GD3 ganglioside expressed cell-inhibitory effects resulting in a decreased heat production rate over 2-18 h of incubation. The 4.2 monoclonal IgM antibody to GD3 had no similar cell-inhibitory effect. In contrast, the 4.2 antibody expressed a much stronger effect than 2B2 in tests for complement-dependent cytotoxicity. The kinetics of these effects were quite reproducible. It is concluded that microcalorimetry is a sensitive and particularly suitable method for the analysis of cytotoxicity kinetics.
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| 17. |
- Baldetorp, Bo, et al.
(författare)
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Statistical evaluation of cell kinetic data from DNA flow cytometry (FCM) by the EM algorithm
- 1989
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Ingår i: Cytometry. - : Wiley. - 0196-4763 .- 1097-0320. ; 10:6, s. 695-705
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Tidskriftsartikel (refereegranskat)abstract
- Flow cytometric DNA measurements yield the amount of DNA for each of a large number of cells. A DNA histogram normally consists of a mixture of one or more constellations of G0/G1-, S-, G2/M-phase cells, together with internal standards, debris, background noise, and one or more populations of clumped cells. We have modelled typical DNA histograms as a mixed distribution with Gaussian densities for the G0/G1 and G2/M phases, an S-phase density, assumed to be uniform between the G0/G1 and G2/M peaks, observed with a Gaussian error, and with Gaussian densities for standards of chicken and trout red blood cells. The debris is modelled as a truncated exponential distribution, and we also have included a uniform background noise distribution over the whole observation interval. We have explored a new approach for maximum-likelihood analyses of complex DNA histograms by the application of the EM algorithm. This algorithm was used for four observed DNA histograms of varying complexity. Our results show that the algorithm works very well, and it converges to reasonable values for all parameters. In simulations from the estimated models, we have investigated bias, variance, and correlations of the estimates.
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| 18. |
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| 19. |
- Berger, Roland, et al.
(författare)
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C-band heteromorphism in breast cancer patients
- 1985
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Ingår i: Cancer Genetics and Cytogenetics. - : Elsevier BV. - 0165-4608. ; 18:1, s. 37-42
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Tidskriftsartikel (refereegranskat)abstract
- The pattern of heteromorphism in the C-band-positive constitutive heterochromatin of human chromosomes #1, #9, and #16 was studied in peripheral lymphocytes of 54 breast cancer patients and 78 control individuals. The parameters of the heterochromatic regions analyzed were relative size, symmetry-asymmetry within homologous pairs, and prevalence of inversions. Significant differences between the two groups were found in C-band size of chromosomes #1, #9, and #16 and in incidence of inversions on chromosomes #1 and #9. Significant differences were noted between premenopausal and postmenopausal cancer patients in regard to inversions on chromosome #9 and between familial and sporadic patients in regard to C-band size on chromosome #16.
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| 20. |
- Blomqvist, G, et al.
(författare)
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Differences in lodgement of tumour cells in muscle and liver
- 1988
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Ingår i: Clinical and Experimental Metastasis. - 1573-7276. ; 6:4, s. 285-289
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Tidskriftsartikel (refereegranskat)abstract
- Differences in the lodgement of circulating tumour cells in various organs are considered an important factor in metastatic organ selection. The present vital microscopic studies show that the pattern of intravascular arrest of tumour cells in muscle after intra-arterial injection is similar to that observed earlier, in the liver, after intraportal injection. However, parallel isotope studies on the lodgement process (at 5 min and 3 h after injection) showed that the tumour cells trapped in the muscle microvasculature were destroyed at a higher rate than in the liver. Tumour cells kept in test tubes, and thus not being subjected to the shearing forces of the circulation, had a higher survival rate than cells trapped in the muscle. The results indicate that stronger retardation forces acting on the tumour cells in muscle (arterial dissemination) than in the liver (venous dissemination) may be one mechanism behind the increased tumour cell destruction in muscle.
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